* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.
Stage #1: With N-ethyl-N,N-diisopropylamine In dichloromethane for 3 h; Stage #2: With piperidine In N,N-dimethyl-formamide for 0.1 h;
General procedure: 4.2 General procedure A: resin loading (0026) Solid phase peptide synthesis was conducted manually in a sinter-fitted polypropylene syringe. 2-Chlorotritylchloride (CTC) resin was preswelled in DCM (mL) for 15min and drained. The first amino acid in 0.4M DIPEA/DCM was added and the mixture was agitated for 3h. After draining the solvent, any free 2-CTC resin linkers were capped by treatment of the resin with a solution of 17:2:1 DCM/MeOH/DIPEA (3×3mL×5min), and subsequently with a solution of 8:1:1 DMF/DIPEA/acetic anhydride (2×3mL×10min). The resin was finally washed with DCM (2×3mL×1min), DMF (2×3mL×1min), DCM (2×3mL×1min) and DMF (2×3mL×1min). 4.3 General procedure B: Fmoc deprotection (0027) The resin was agitated with a solution of 10percent piperidine inDMF (2×3mL×3min) and subsequently washed with DMF(3×3mL×1min), DCM (3×3mL×1min), DMF (5×3mL×1min). The deprotected solutions were combined and diluted appropriately (100-fold for 0.05mmol resin loading). The resin loading was estimated by measuring the absorbance of the piperidine-fulvene adduct with 10percent piperidine in DMF as a reference (λ=301nm; ε=7800M−1cm−1). 4.4 General procedure C: peptide coupling with HBTU (0028) A solution was prepared of the appropriate Fmoc-protected amino acid (3 equiv. relative to resin loading) and HBTU (2.9 equiv. relative to resin loading) in minimum amount of DMF. DIPEA (6 equiv. relative to resin loading) was added and the resin was agitated for 1.5h. The resin was then drained and washed with DMF (3×3mL×1min), DCM (3×3mL×1min) and DMF (5×3mL×1min). 4.5 General procedure D: peptide coupling with HATU (0029) A solution was prepared of the appropriate Fmoc-protected amino acid (3 equiv. relative to resin loading) and HATU (2.9 equiv. relation to resin loading) in minimal DMF. DIPEA (6 equiv. relation to resin loading) was added to the solution and the mixture was immediately added to the resin and agitated. Reaction times were altered based on the residue being coupled: Phe(NMe) and Ala (2×2h); Thr and Sta (1×2h); Asn, Leu, D-Val and L-Val (2×2h); DMVal (3×3h). Once the reaction was complete, the resin was drained and washed with DMF (3×3mL×1min), DCM (3×3mL×1min) and DMF (3×3mL×1min). 4.6 General procedure E: peptide coupling with DIC (0030) A solution was prepared of the appropriate Fmoc-protected amino acid (1.5 equiv. relative to resin loading), HOBt (1.5 equiv. relative to resin loading) and DIC (1.5 equiv. relative to resin loading) in minimal DMF. This solution was stirred for 20min, then added to the resin and agitated overnight. The resin was drained and washed with DMF (3×3mL×1min), DCM (3×3mL×1min) and DMF (5×3mL×1min). Double coupling of the next amino acid after the coupling of the fluorinated amino acid was applied. 4.7 General procedure F: resin cleavage (0031) After the last Fmoc deprotection, the resin was washed with DMF (3×3mL×1min) and DCM (3×3mL×1min) then dried in vacuo. The resin was agitated with a solution of 95:2.5:2.5 TFA/TIS/H2O (3mL) for 2h. The resin was drained and washed with the same TFA mixture above (2×3mL×1min). The combined cleavage solutions were concentrated under a stream of nitrogen. Diethyl ether was added and the supernatant was decanted (3×). The residue was then dried in vacuo to provide the crude linear peptide
3-[((S)-2-{(3S,4S)-4-[(S)-2-((S)-2-Acetylamino-3-methyl-butyrylamino)-propionylamino]-3-hydroxy-6-methyl-heptanoylamino}-3-methyl-butyrylamino)-methyl]-benzoic acid[ No CAS ]
N-(9-fluorenylmethoxycarbonyl)-D-leucine[ No CAS ]
[ 158257-40-0 ]
[ 155369-11-2 ]
3-[((S)-2-{(3S,4S)-4-[(S)-2-((R)-2-Acetylamino-4-methyl-pentanoylamino)-propionylamino]-3-hydroxy-6-methyl-heptanoylamino}-3-methyl-butyrylamino)-methyl]-benzoic acid[ No CAS ]
3-[((S)-2-{(3S,4S)-4-[(S)-2-((S)-2-Acetylamino-hexanoylamino)-propionylamino]-3-hydroxy-6-methyl-heptanoylamino}-3-methyl-butyrylamino)-methyl]-benzoic acid[ No CAS ]
3-[((S)-2-{(3S,4S)-4-[(S)-2-((S)-2-Acetylamino-pentanoylamino)-propionylamino]-3-hydroxy-6-methyl-heptanoylamino}-3-methyl-butyrylamino)-methyl]-benzoic acid[ No CAS ]
3-({(S)-2-[(3S,4S)-3-Hydroxy-6-methyl-4-((S)-2-{(2S,3S)-3-methyl-2-[(tetrahydro-pyran-2-carbonyl)-amino]-pentanoylamino}-propionylamino)-heptanoylamino]-3-methyl-butyrylamino}-methyl)-benzoic acid[ No CAS ]
3-[((S)-2-{(3S,4S)-4-[(S)-2-((2S,3S)-2-Acetylamino-3-methyl-pentanoylamino)-propionylamino]-3-hydroxy-6-methyl-heptanoylamino}-3-methyl-butyrylamino)-methyl]-benzoic acid[ No CAS ]
3-[((S)-2-{(3S,4S)-4-[(S)-2-((2S,3S)-2-Acetylamino-3-methyl-pentanoylamino)-propionylamino]-3-hydroxy-6-methyl-heptanoylamino}-4-methyl-pentanoylamino)-methyl]-benzoic acid[ No CAS ]
3-[(S)-2-{(3S,4S)-4-[(S)-2-((2S,3S)-2-Acetylamino-3-methyl-pentanoylamino)-propionylamino]-3-hydroxy-6-methyl-heptanoylamino}-3-(4-hydroxy-phenyl)-propionylamino]-methyl}-benzoic acid[ No CAS ]
3-[((S)-2-{(3S,4S)-4-[(S)-2-((2S,3S)-2-Acetylamino-3-methyl-pentanoylamino)-propionylamino]-3-hydroxy-6-methyl-heptanoylamino}-3-phenyl-propionylamino)-methyl]-benzoic acid[ No CAS ]
3-[((S)-2-{(3S,4S)-4-[(S)-2-((2S,3S)-2-Acetylamino-3-methyl-pentanoylamino)-propionylamino]-3-hydroxy-6-methyl-heptanoylamino}-hexanoylamino)-methyl]-benzoic acid[ No CAS ]
3-[(S)-2-{(3S,4S)-4-[(S)-2-((2S,3S)-2-Acetylamino-3-methyl-pentanoylamino)-4-methylsulfanyl-butyrylamino]-3-hydroxy-6-methyl-heptanoylamino}-3-(4-hydroxy-phenyl)-propionylamino]-methyl}-benzoic acid[ No CAS ]
3-[((S)-2-{(3S,4S)-4-[(S)-2-((2S,3S)-2-Acetylamino-3-methyl-pentanoylamino)-4-methylsulfanyl-butyrylamino]-3-hydroxy-6-methyl-heptanoylamino}-3-phenyl-propionylamino)-methyl]-benzoic acid[ No CAS ]
3-[((S)-2-{(3S,4S)-4-[(S)-2-((2S,3S)-2-Acetylamino-3-methyl-pentanoylamino)-4-methylsulfanyl-butyrylamino]-3-hydroxy-6-methyl-heptanoylamino}-hexanoylamino)-methyl]-benzoic acid[ No CAS ]
3-[((S)-2-{(3S,4S)-4-[(S)-2-((2S,3S)-2-Acetylamino-3-methyl-pentanoylamino)-4-methylsulfanyl-butyrylamino]-3-hydroxy-6-methyl-heptanoylamino}-pentanoylamino)-methyl]-benzoic acid[ No CAS ]
3-[((S)-2-{(3S,4S)-4-[(S)-2-((S)-2-Acetylamino-4-methyl-pentanoylamino)-propionylamino]-3-hydroxy-6-methyl-heptanoylamino}-3-methyl-butyrylamino)-methyl]-benzoic acid[ No CAS ]
N-(9-fluorenylmethoxycarbonyl)-D-alanine[ No CAS ]
[ 158257-40-0 ]
[ 155369-11-2 ]
3-[((S)-2-{(3S,4S)-4-[(R)-2-((S)-2-Acetylamino-4-methyl-pentanoylamino)-propionylamino]-3-hydroxy-6-methyl-heptanoylamino}-3-methyl-butyrylamino)-methyl]-benzoic acid[ No CAS ]
3-[((S)-2-{(3S,4S)-4-[(S)-2-((S)-2-Acetylamino-4-methyl-pentanoylamino)-4-methylsulfanyl-butyrylamino]-3-hydroxy-6-methyl-heptanoylamino}-3-methyl-butyrylamino)-methyl]-benzoic acid[ No CAS ]
3-[((S)-2-{(3S,4S)-4-[(R)-2-((S)-2-Acetylamino-4-methyl-pentanoylamino)-4-methylsulfanyl-butyrylamino]-3-hydroxy-6-methyl-heptanoylamino}-3-methyl-butyrylamino)-methyl]-benzoic acid[ No CAS ]
3-[((R)-2-{(3S,4S)-4-[(S)-2-((S)-2-Acetylamino-4-methyl-pentanoylamino)-4-methylsulfanyl-butyrylamino]-3-hydroxy-6-methyl-heptanoylamino}-3-methyl-butyrylamino)-methyl]-benzoic acid[ No CAS ]
Stage #1: N-Fmoc-statine With N-ethyl-N,N-diisopropylamine In dichloromethane for 3h;
Stage #2: With piperidine In N,N-dimethyl-formamide for 0.1h;
Stage #3: 3-methylbutyric acid; Fmoc-Val-OH; N-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-alanine; N-Fmoc-statine Further stages;
General procedure: 4.2 General procedure A: resin loading (0026) Solid phase peptide synthesis was conducted manually in a sinter-fitted polypropylene syringe. 2-Chlorotritylchloride (CTC) resin was preswelled in DCM (mL) for 15min and drained. The first amino acid in 0.4M DIPEA/DCM was added and the mixture was agitated for 3h. After draining the solvent, any free 2-CTC resin linkers were capped by treatment of the resin with a solution of 17:2:1 DCM/MeOH/DIPEA (3×3mL×5min), and subsequently with a solution of 8:1:1 DMF/DIPEA/acetic anhydride (2×3mL×10min). The resin was finally washed with DCM (2×3mL×1min), DMF (2×3mL×1min), DCM (2×3mL×1min) and DMF (2×3mL×1min). 4.3 General procedure B: Fmoc deprotection (0027) The resin was agitated with a solution of 10% piperidine inDMF (2×3mL×3min) and subsequently washed with DMF(3×3mL×1min), DCM (3×3mL×1min), DMF (5×3mL×1min). The deprotected solutions were combined and diluted appropriately (100-fold for 0.05mmol resin loading). The resin loading was estimated by measuring the absorbance of the piperidine-fulvene adduct with 10% piperidine in DMF as a reference (λ=301nm; ε=7800M-1cm-1). 4.4 General procedure C: peptide coupling with HBTU (0028) A solution was prepared of the appropriate Fmoc-protected amino acid (3 equiv. relative to resin loading) and HBTU (2.9 equiv. relative to resin loading) in minimum amount of DMF. DIPEA (6 equiv. relative to resin loading) was added and the resin was agitated for 1.5h. The resin was then drained and washed with DMF (3×3mL×1min), DCM (3×3mL×1min) and DMF (5×3mL×1min). 4.5 General procedure D: peptide coupling with HATU (0029) A solution was prepared of the appropriate Fmoc-protected amino acid (3 equiv. relative to resin loading) and HATU (2.9 equiv. relation to resin loading) in minimal DMF. DIPEA (6 equiv. relation to resin loading) was added to the solution and the mixture was immediately added to the resin and agitated. Reaction times were altered based on the residue being coupled: Phe(NMe) and Ala (2×2h); Thr and Sta (1×2h); Asn, Leu, D-Val and L-Val (2×2h); DMVal (3×3h). Once the reaction was complete, the resin was drained and washed with DMF (3×3mL×1min), DCM (3×3mL×1min) and DMF (3×3mL×1min). 4.6 General procedure E: peptide coupling with DIC (0030) A solution was prepared of the appropriate Fmoc-protected amino acid (1.5 equiv. relative to resin loading), HOBt (1.5 equiv. relative to resin loading) and DIC (1.5 equiv. relative to resin loading) in minimal DMF. This solution was stirred for 20min, then added to the resin and agitated overnight. The resin was drained and washed with DMF (3×3mL×1min), DCM (3×3mL×1min) and DMF (5×3mL×1min). Double coupling of the next amino acid after the coupling of the fluorinated amino acid was applied. 4.7 General procedure F: resin cleavage (0031) After the last Fmoc deprotection, the resin was washed with DMF (3×3mL×1min) and DCM (3×3mL×1min) then dried in vacuo. The resin was agitated with a solution of 95:2.5:2.5 TFA/TIS/H2O (3mL) for 2h. The resin was drained and washed with the same TFA mixture above (2×3mL×1min). The combined cleavage solutions were concentrated under a stream of nitrogen. Diethyl ether was added and the supernatant was decanted (3×). The residue was then dried in vacuo to provide the crude linear peptide
General procedure: 4.2 General procedure A: resin loading (0026) Solid phase peptide synthesis was conducted manually in a sinter-fitted polypropylene syringe. 2-Chlorotritylchloride (CTC) resin was preswelled in DCM (mL) for 15min and drained. The first amino acid in 0.4M DIPEA/DCM was added and the mixture was agitated for 3h. After draining the solvent, any free 2-CTC resin linkers were capped by treatment of the resin with a solution of 17:2:1 DCM/MeOH/DIPEA (3×3mL×5min), and subsequently with a solution of 8:1:1 DMF/DIPEA/acetic anhydride (2×3mL×10min). The resin was finally washed with DCM (2×3mL×1min), DMF (2×3mL×1min), DCM (2×3mL×1min) and DMF (2×3mL×1min). 4.3 General procedure B: Fmoc deprotection (0027) The resin was agitated with a solution of 10% piperidine inDMF (2×3mL×3min) and subsequently washed with DMF(3×3mL×1min), DCM (3×3mL×1min), DMF (5×3mL×1min). The deprotected solutions were combined and diluted appropriately (100-fold for 0.05mmol resin loading). The resin loading was estimated by measuring the absorbance of the piperidine-fulvene adduct with 10% piperidine in DMF as a reference (lambda=301nm; epsilon=7800M-1cm-1). 4.4 General procedure C: peptide coupling with HBTU (0028) A solution was prepared of the appropriate Fmoc-protected amino acid (3 equiv. relative to resin loading) and HBTU (2.9 equiv. relative to resin loading) in minimum amount of DMF. DIPEA (6 equiv. relative to resin loading) was added and the resin was agitated for 1.5h. The resin was then drained and washed with DMF (3×3mL×1min), DCM (3×3mL×1min) and DMF (5×3mL×1min). 4.5 General procedure D: peptide coupling with HATU (0029) A solution was prepared of the appropriate Fmoc-protected amino acid (3 equiv. relative to resin loading) and HATU (2.9 equiv. relation to resin loading) in minimal DMF. DIPEA (6 equiv. relation to resin loading) was added to the solution and the mixture was immediately added to the resin and agitated. Reaction times were altered based on the residue being coupled: Phe(NMe) and Ala (2×2h); Thr and Sta (1×2h); Asn, Leu, D-Val and L-Val (2×2h); DMVal (3×3h). Once the reaction was complete, the resin was drained and washed with DMF (3×3mL×1min), DCM (3×3mL×1min) and DMF (3×3mL×1min). 4.6 General procedure E: peptide coupling with DIC (0030) A solution was prepared of the appropriate Fmoc-protected amino acid (1.5 equiv. relative to resin loading), HOBt (1.5 equiv. relative to resin loading) and DIC (1.5 equiv. relative to resin loading) in minimal DMF. This solution was stirred for 20min, then added to the resin and agitated overnight. The resin was drained and washed with DMF (3×3mL×1min), DCM (3×3mL×1min) and DMF (5×3mL×1min). Double coupling of the next amino acid after the coupling of the fluorinated amino acid was applied. 4.7 General procedure F: resin cleavage (0031) After the last Fmoc deprotection, the resin was washed with DMF (3×3mL×1min) and DCM (3×3mL×1min) then dried in vacuo. The resin was agitated with a solution of 95:2.5:2.5 TFA/TIS/H2O (3mL) for 2h. The resin was drained and washed with the same TFA mixture above (2×3mL×1min). The combined cleavage solutions were concentrated under a stream of nitrogen. Diethyl ether was added and the supernatant was decanted (3×). The residue was then dried in vacuo to provide the crude linear peptide. 4.8 General procedure G: resin cleavage and esterification (0032) Acetyl chloride (0.63mL, 9.0mmol) was added dropwise to MeOH (3mL) at 0C, and the solution was stirred on ice for 2h prior to use in the cleavage reaction. Peptides bound to the resin were swelled with DCM (1mL) for 30min and the methanolic HCl solution was then added in a three to four-fold volumetric excess. The reaction was agitated for 2h and then drained and washed with MeOH (3×3mL×1min). The solution was evaporated and then dried under high vacuum for 1h before addition of TFA (1mL) followed by stirring at RT for 1h. The remaining TFA was evaporated under a stream of N2 and the crude residue was washed with diethyl ether (3×1mL) before purification by HPLC.
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