The ARF (ADP-ribosylation factor) family is a group of small GTPases, including several subtypes such as ARF1, ARF2, and ARF6. They consist of a GTP/GDP binding domain and are primarily located in the cell membrane and the Golgi apparatus. The ARF family is involved in regulating vesicle transport, cell cytoskeleton reorganization, and membrane fluidity. By binding and hydrolyzing GTP, they activate or inhibit downstream effector proteins, affecting cellular endocytosis and secretion processes. The expression of the ARF family is tissue- and cell-specific, and mutations or dysregulation of their genes are associated with various diseases, including cancer, neurodegenerative diseases, and cardiovascular diseases.