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CAS No. : | 56180-94-0 | MDL No. : | MFCD00869592 |
Formula : | C25H43NO18 | Boiling Point : | - |
Linear Structure Formula : | - | InChI Key : | - |
M.W : | 645.60 | Pubchem ID : | - |
Synonyms : |
BAY g 5421
|
Chemical Name : | (2R,3R,4R,5R)-4-(((2R,3R,4R,5S,6R)-5-(((2R,3R,4S,5S,6R)-3,4-Dihydroxy-6-methyl-5-(((1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl)amino)tetrahydro-2H-pyran-2-yl)oxy)-3,4-dihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-2,3,5,6-tetrahydroxyhexanal |
Signal Word: | Warning | Class: | N/A |
Precautionary Statements: | P261-P305+P351+P338 | UN#: | N/A |
Hazard Statements: | H315-H319-H335 | Packing Group: | N/A |
GHS Pictogram: |
* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With recombinant amylopullulanase from hyperthermophilic archaeon Caldivirga maquilingensis In aq. acetate buffer at 95℃; Enzymatic reaction; | 2.6. Hydrolysis of various substrates by CMApu General procedure: α-CD, β-CD, γ-CD, G1-β-CD, G2-β-CD, G8-β-CD, G3 and acarbose were used as the substrates of CMApu to examine the hydrolytic patterns. A-50 μL of CMApu (0.8 U) was incubated with 125 μL of 1% (w/v) substrate, 62.5 μL of 200 mM sodium acetate buffer (pH 5.0) and 12.5 μL of double distilled water at 95°C for various time. The hydrolytic products were analysed by thin-layer chromatography (TLC). Normal starch, amylose, and amylopectin from potato were used as the substrates of CMApu to prepare the linear maltodextrins. To obtain 1% (w/v) amylose solution, amylose was dissolved with 1 M NaOH, then diluted 10 times with double distilled water, and finally neutralized with 0.1 M HCl (Park et al., 2007). For the hydrolysis of amylose, a-10 μL of CMApu (0.16 U) was incubated with 200 μL of amylose, 250 μL of 200 mM sodium acetate buffer (pH 5.0) and 540 μL of double distilled water at 100°C for 1 h. For the hydrolysis of amylopectin or starch, a-10 μL of CMApu (0.16 U) was incubated with 2 mg of amylopectin or starch, and 90 μL of 50 mM sodium acetate buffer (pH 5.0) at 100°C for 1 h. The reaction solutions (0.2%, w/v) were passed through a 0.2-μm disposable syringe filter, and then the hydrolytic products were analysed by a high-performance anion exchange chromatography (HPAEC). |