Products Cited in Publications Worldwide Metatranscriptomics-guided discovery and characterization of a polyphenol-metabolizing gut microbial enzyme

Metatranscriptomics-guided discovery and characterization of a polyphenol-metabolizing gut microbial enzyme

Minwoo Bae; Chi Le (Chip); Raaj S. Mehta; Xueyang Dong; Lindsey M. Pieper; Lorenzo Ramirez; Margaret Alexander; Sina Kiamehr; Peter J. Turnbaugh; Curtis Huttenhower; Andrew T. Chan; Emily P. Balskus

DOI: PMID:

Abstract

Gut microbial catechol dehydroxylases are a largely uncharacterized family of metalloenzymes that potentially impact human health by metabolizing dietary polyphenols. Here, we use metatranscriptomics (MTX) to identify highly transcribed catechol-dehydroxylase-encoding genes in human gut microbiomes. We discover a prevalent, previously uncharacterized catechol dehydroxylase (Gp Hcdh) from Gordonibacter pamelaeae that dehydroxylates hydrocaffeic acid (HCA), an anti-inflammatory gut microbial metabolite derived from plant-based foods. Further analyses suggest that the activity of Gp Hcdh may reduce anti-inflammatory benefits of polyphenol-rich foods. Together, these results show the utility of combining MTX analysis and biochemical characterization for gut microbial enzyme discovery and reveal a potential link between host inflammation and a specific polyphenol-metabolizing gut microbial enzyme.

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Chlorogenic acid

A163338327-97-998%

Chlorogenic acid

12.0/250mg

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