Products Cited in Publications Worldwide Peroxidase mimetic BSA-stabilized Pt nanozyme for colorimetric determination of arsenic

Peroxidase mimetic BSA-stabilized Pt nanozyme for colorimetric determination of arsenic

Karataş, Büşra; Kara, Hayriye Eda Şatana

DOI:

Abstract

Arsenic is a highly toxic metal with harmful properties for the environment, humans, and other living things. Nanomaterials that exhibit enzyme-like catalytic activity are called nanozymes. In this study, BSA-stabilized Pt nanozymes were synthesized and characterized using TEM, XPS, and Uv-vis spectroscopic methods. A peroxidase mimetic nanozymes were used for the determination of arsenic in water, urine, and serum samples based on nanoparticle-catalyzed oxidation of colorless (TMB) to blue oxTMB in the presence of H2O2. Arsenic selectively inhibited this oxidation process, causing a decrease in UV–Vis absorbance at 652 nm and solution discoloration. The average diameter of Pt nanoparticles was measured as 1–2 nm and possesses highly peroxidase-like activity with the Km values of 0.17 mM and 104 mM towards and H2O2, respectively. Under optimized conditions, arsenic was detected with a detection limit of 0.75 nM with a linear response range of 10–100 nM, which means the developed system is sensitive enough to detect As in drinking water, which has the maximum allowable limits of 10 μg/L (133 nM) defined by the World Health Organization (WHO) and the EPA. Recovery values were obtained 99 %–100.4 %–105.6 % for water, urine, and serum samples, respectively. AGREE calculator assessment (0.72), BAGI (72.5), and RAPI (90.0) confirmed the method's greenness, practical applicability, and offering advantages of minimal organic solvent consumption compared to existing techniques.

Keywords

Nanozyme ; Nanoparticle ; Platinum ; Arsenic ; Colorimetric detection

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Dipotassium tetrachloroplatinate

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Dipotassium tetrachloroplatinate

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TMB

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