Products Cited in Publications Worldwide AMP-activated protein kinase mediates (-)-epigallocatechin-3-gallate (EGCG) to promote lipid synthesis in mastitis cows

AMP-activated protein kinase mediates (-)-epigallocatechin-3-gallate (EGCG) to promote lipid synthesis in mastitis cows

Hu, Chunli; Dou, Wenli; Ma, Xuehu; An, Yanhao; Wang, Dezhi; Ma, Yanfen

DOI: PMID:

Abstract

Mastitis, a serious threat to the health and milk production function of dairy cows decreasesmilk quality. Blood from three healthy cows and three mastitis cows were collected in thisstudy and their transcriptome was sequenced using the Illumina HiSeq platform. Differentiallyexpressed genes (DEGs) were screened according to the |log2FoldChange| > 1 and P-value <0.05 criteria. Pathway enrichment and functional annotation were performed through KEGGand GO analyses. Finally, the mechanism of the AMP-activated protein kinase (AMPK)mediation of (-)-epigallocatechin-3-gallate (EGCG) to promote lipid metabolism in mastitiscows was analyzed in bovine mammary epithelial cells (BMECs). Transcriptome analysisrevealed a total of 825 DEGs, with 474 genes showing increased expression and 351 genesshowing decreased expression. The KEGG analysis of DEGs revealed that they were mainlylinked to tumour necrosis factor, nuclear factor-κB signalling pathway, and lipidmetabolism-related signalling pathway, whereas GO functional annotation found that DEGswere enriched in threonine and methionine kinase activity, cellular metabolic processes, andcytoplasm. AMPK expression, which is involved in several lipid metabolism pathways, wasdownregulated in mastitis cows. The results of in vitro experiments showed that the inhibitionof AMPK promoted the expression of lipid synthesis genes in lipopolysaccharide-inducedBMECs and that EGCG could promote lipid synthesis by decreasing the expression of AMPKand downregulating the expression of inflammatory factors in inflammatory BMECs. Inconclusion, our study demonstrated that AMPK mediated EGCG to inhabit of inflammatoryresponses and promote of lipid synthesis in inflammatory BMECs.

Keywords

transcriptome ; AmPK ; egcg ; mastitis ; cow

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