Products Cited in Publications Worldwide Ganoderma lucidum ekstraktından mikosentez yoluyla metal oksit nanopartikül sentezi ve bazı biyolojik aktivitelerinin belirlenmesi

Ganoderma lucidum ekstraktından mikosentez yoluyla metal oksit nanopartikül sentezi ve bazı biyolojik aktivitelerinin belirlenmesi

Tümer, Meltem

Abstract

Chemical, physical and biological methods are used in the synthesis of metal oxide nanoparticles, which have the potential to be used in many fields such as medical, food, textile and treatment. Biological methods using plants and microorganisms are also called green synthesis. Mycosynthesis is a biological synthesis method using fungi. In this thesis, myosynthesis of zinc oxide (ZnO NP) and copper oxide (CuO NP) nanoparticles from Ganoderma lucidum mushroom extract was carried out, characterised and some biological activities were investigated. Aqueous extracts of medically important G. lucidum pellets were used as reducing agent in the synthesis process, ZnO NPs and CuO NPs were mycosynthesised at 25 ⁰C and characterised by UV-visible (UV-vis) spectrophotometry, Fourier transform infrared spectrometry (FTIR), scanning electron microscopy (SEM), X-ray spectrometry (EDS), transmission electron microscopy (TEM) and X-ray diffraction (XRD). According to the results, it was observed that the mycosynthesised ZnO NPs were spherical, CuO NPs were regular polyhedral shapes and gave surface plasmon resonance (SPR) bands at 303 nm and 275 nm, respectively. ZnO NPs and CuO NPs were observed to have a particle size distribution in the range of 1-2 nm and 20-40 nm, respectively. In order to evaluate some biological activities of the mycosynthesised ZnO and CuO NPs, antimicrobial activities, activities and antidiabetic activities were investigated. According to the results, the % inhibition values of mycosynthesised ZnO and CuO NPs at 100 μg/mL concentration were 81.46±1.12% and 84.23±0.69%, respectively. ZnO and CuO NPs showed activity against Escherichia coli and Staphylococcus aureus and activity against Candida albicans. Finally, the antidiabetic activities of the synthesised NPs were investigated under in vitro conditions and it was found that ZnO NPs at a concentration of 100 μg/mL inhibited and α-glucosidase by 69.02±0.49% and 63.84±0.99%, respectively, while CuO NPs at a concentration of 100 μg/mL inhibited them by 72.84±0.32% and 76.01±0.05%, respectively. According to the results, it is thought that the myosynthesised ZnO and CuO NPs have , antimicrobial and antihyperglycaemic effects and have the potential to be used in health, pharmaceutical and biomedical fields.

Keywords

Ganoderma lucidum ; metal oxide nanoparticle ; mycosynthesis ; ; antimicrobial ; antidiabetic

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Acarbose

A35760356180-94-098%

Acarbose

10.0/1mg

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