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Chemical Structure| 2002-60-0 Chemical Structure| 2002-60-0

Structure of 2002-60-0

Chemical Structure| 2002-60-0

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Product Details of [ 2002-60-0 ]

CAS No. :2002-60-0
Formula : C5H4N4O2S
M.W : 184.18
SMILES Code : S=C(NC(N1)=C2NC1=O)NC2=O
English Name :2-Thioxo-2,3,7,9-tetrahydro-1H-purine-6,8-dione
MDL No. :MFCD00871830

Safety of [ 2002-60-0 ]

Application In Synthesis of [ 2002-60-0 ]

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

  • Downstream synthetic route of [ 2002-60-0 ]

[ 2002-60-0 ] Synthesis Path-Downstream   1~1

  • 1
  • [ 2002-59-7 ]
  • [ 2002-60-0 ]
YieldReaction ConditionsOperation in experiment
With human xanthine oxidase from escherichia coli lysate In aq. phosphate buffer; dimethyl sulfoxide Enzymatic reaction; General procedure: Incubation Conditions. The incubation mixture consisted of substrates 6MP or 6TX at a final concentration in the range of 10-1000 μM and 0.1-200 μM, respectively, in 25 mM potassium phosphate buffer (pH 7.4) containing 0.1 mM EDTA. The final concentration of dimethylsulfoxide was 0.5% (v/v). For the inhibition experiments, a substrate concentration of 100 μM was used. Inhibitors raloxifene and febuxostat were used at a final concentration of 1 μM.The substrate and inhibitor stocks were made up so that the total concentration of dimethylsulfoxide in the reaction mixture was 1.5% (v/v). All incubations for 6TX and 6TUA formation were allowed to proceed at 37°C for 15 and 30 minutes, respectively. The formation of 6TX was observed to be linear with respect to time for 15 minutes and 6TUA for more than 30 minutes. The reaction was initiated by addition of pooled human liver cytosol (HLC) ata final concentration of 0.5 or 1 mg/ml and a known concentration of purified human AO and human XO lysate. The final reaction volume was 80 ml. The reaction was quenched by the addition of 20 ml of 1 M formic acid containing 1 μM 3,5 dibromo-4-hydroxybenzoic acid as internal standard. The samples were then centrifuged at 5000 rpm for 10 minutes in an Eppendorf centrifuge 5415D and the supernatant was collected for analysis.
 

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