The SRPK family is a group of serine/arginine protein kinases, primarily including members like SRPK1 and SRPK2, possessing the capability for the specific phosphorylation of RNA processing proteins. These kinases have a structural composition that includes a catalytic domain and two N-terminal regulatory regions. They mainly localize in the cell nucleus and cytoplasm, regulating the activity of splicing factors, thereby participating in RNA splicing and other gene expression regulatory processes. The expression of the SRPK family is tissue-specific, and alterations in their activity or gene mutations are associated with the occurrence of various diseases, including cancer and neurodegenerative disorders. These kinases play a crucial role in cellular signal transduction and gene expression regulation.