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O95760-Human IL-33-3d structure.jpg O95760-Human IL-33-3d structure.jpg

Human IL-33+SDS-PAGE.jpg Human IL-33+SDS-PAGE.jpg Greater than 95% as determined by reducing SDS-PAGE.

Greater than 95% as determined by reducing SDS-PAGE.

Human IL-33+ELISA.jpg Human IL-33+ELISA.jpg Measured by ELISA, Mouse ST2 binds to Human IL-33 with an ED50 of 0.63 μg/ml.

Measured by ELISA, Mouse ST2 binds to Human IL-33 with an ED50 of 0.63 μg/ml.

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Recombinant Human IL-33 is a human-origin interleukin-33 protein expressed in E. coli without tags. As a pro-inflammatory nuclear factor of the IL-1 family, it exhibits both intracellular and extracellular activities. The full-length protein consists of 270 amino acids and is predominantly expressed in human high endothelial venule endothelial cells. Its C-terminal mature fragment can bind and activate the ST2 receptor, triggering NF-κB and MAPK signaling pathways to induce the production of Th2-type cytokines, which are closely associated with mucosal immune pathology.

Synonyms: Recombinant Human IL-33; Interleukin-33; IL-33

4.5 *For Research Use Only! Not for Human Use. We Do Not Sell to Patients.

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Product Details of Human IL-33

M.W : 18.1 KDa
SMILES Code : NONE
Synonyms :
Recombinant Human IL-33; Interleukin-33; IL-33
English Name :Recombinant Human IL-33

Safety of Human IL-33

Isoform Comparison

Biological Activity

Description
Interleukin-33 (IL-33) was initially discovered as a nuclear factor NF-HEV abundantly expressed in high endothelial venules. It is a 30-32 kD pro-inflammatory protein with intracellular and extracellular activities and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is highly and selectively expressed by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus, and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling. IL-33 mediates its biological effects via Toll-interleukin 1 (IL-1) receptor (TIR) domain-containing receptor ST2, activates NF-kappaB and MAP kinases, and drives production of T(H)2-associated cytokines from in vitro polarized T(H)2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. Human IL-33 is 270 amino acids in length.

In Vitro:

Cell Line
Concentration Treated Time Description References
T84 cells 10 ng/mL 48 hours To evaluate the effect of IL-33 on epithelial barrier function. Results showed that IL-33 augmented TER in T84 cells via ERK1/2 signaling pathway. Inflamm Bowel Dis. 2015 Dec;21(12):2737-46
 

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