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Chemical Structure| 188404-10-6 Chemical Structure| 188404-10-6

Structure of MAFP
CAS No.: 188404-10-6

Chemical Structure| 188404-10-6

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MAFP is a potent, irreversible inhibitor of fatty acid amide hydrolase (FAAH, anandamide amidase), the enzyme responsible for anandamide hydrolysis (IC50 = 2.5 nM). MAFP also binds irreversibly to CB1 receptors with IC50 of 20 nM.

Synonyms: Methyl Arachidonyl Fluorophosphonate

4.5 *For Research Use Only! Not for Human Use. We Do Not Sell to Patients.

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Product Details of MAFP

CAS No. :188404-10-6
Formula : C21H36FO2P
M.W : 370.48
SMILES Code : CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCCP(OC)(F)=O
Synonyms :
Methyl Arachidonyl Fluorophosphonate
English Name :(all-Z)-5,8,11,14-Eicosatetraenylphosphonofluoridic Acid Methyl Ester
MDL No. :MFCD03095722
InChI Key :KWKZCGMJGHHOKJ-ZKWNWVNESA-N
Pubchem ID :10429254

Safety of MAFP

Related Pathways of MAFP

GPCR

Isoform Comparison

Biological Activity

In Vitro:

Cell Line
Concentration Treated Time Description References
human coronary artery endothelial cells 10 μM 5-10 minutes Inhibition of 2-AG hydrolysis by MAFP attenuated 2-AG-induced Ca2+ response by about 30%, but the residual Ca2+ response was reversed by HC067047 Br J Pharmacol. 2015 Nov;172(22):5251-64

In Vivo:

Species
Animal Model
Administration Dosage Frequency Description References
Rats PGE2-induced hyperalgesia model Intraplantar injection 2 and 4 μg per paw Single injection, measurements taken over 30 minutes MAFP enhanced the antinociceptive effect induced by PnPP-19 Br J Pharmacol. 2016 May;173(9):1491-501
Rats PGE2-induced hyperalgesia model Intraplantar injection 0.5 mg/paw Single administration, measurements taken at 180 min post-administration MAFP (a dual MAGL/FAAH inhibitor) potentiated the peripheral antinociceptive effect of low-dose kahweol (40 mg/paw). Braz J Med Biol Res. 2021 Oct 29;54(12):e11071

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

2.70mL

0.54mL

0.27mL

13.50mL

2.70mL

1.35mL

26.99mL

5.40mL

2.70mL

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