CAS No. :	1431697-89-0
Formula :	C₂₅H₂₈Cl₂N₄O₂
M.W :	487.42
SMILES Code :	CC(C1=C(N[C@H]2CC[C@H](CN(C)C)CC2)C3=NC(C4=CC(Cl)=C(O)C(Cl)=C4)=CC=C3N=C1)=O
Synonyms :	OTS167
MDL No. :	MFCD23160047
InChI Key :	DKZYXHCYPUVGAF-UHFFFAOYSA-N
Pubchem ID :	135398499

GHS Pictogram:
Signal Word:	Warning
Hazard Statements:	H302-H315-H319-H335
Precautionary Statements:	P261-P305+P351+P338

HEK293T cells	100 nM	48 hours	Evaluate the inhibitory effect of OTS167 on DYRK1A	Bioorg Med Chem. 2020 Jan 1;28(1):115193.
C4-2b cells	30 nM	2 hours	To evaluate the inhibitory effect of OTS167 on MELK activity, results showed that OTS167 inhibited phosphorylation of the known MELK substrate ACC at nanomolar concentrations.	EMBO Mol Med. 2018 Mar;10(3):e8274.
Primary human FLT3 mutant AML blast mononuclear cells	50 nM	24 hours	OTS167 downregulates FLT3 protein expression in primary human FLT3 mutant AML cells.	Blood Cancer J. 2021 Mar 3;11(3):48.
THP-1	50 nM	24 hours	OTS167 downregulates FLT3 protein expression in FLT3 wild-type AML cell lines.	Blood Cancer J. 2021 Mar 3;11(3):48.
SK-N-BE2	0 to 8 nM	24 hours	To evaluate the effects of OTS167 on cell cycle. Results showed that OTS167 at 8 nM significantly decreased the number of cells in G1 phase and increased the number of cells in S phase.	Mol Cancer Ther. 2019 Mar;18(3):507-516.
SW13	0.625 nM, 1.25 nM	24-48 hours	To evaluate the effect of OTS167 on SW13 cell proliferation, results showed that OTS167 had antiproliferative and cytotoxic effects at nanomolar concentrations.	J Exp Clin Cancer Res. 2022 Sep 23;41(1):282.
Recombinant UGT1A3	2.2 µM (Km)	30 minutes	Assessment of UGT1A3 metabolic activity, showing moderate intrinsic clearance (51 ml/min/mg)	Drug Metab Dispos. 2015 Jul;43(7):928-35.
Recombinant UGT1A1	5.7 µM (Km)	30 minutes	Determination of UGT1A1's metabolic contribution, showing highest intrinsic clearance (64 ml/min/mg)	Drug Metab Dispos. 2015 Jul;43(7):928-35.
Human liver microsomes (HLM)	3.4 µM (Km)	30 minutes	Identification of glucuronidation as a metabolic pathway for OTS167, observing formation of a single OTS167-glucuronide (OTS167-G)	Drug Metab Dispos. 2015 Jul;43(7):928-35.
Recombinant UGT1A10	0.9 µM (Km)	35 minutes	Evaluation of UGT1A10 metabolic activity, showing moderate intrinsic clearance (47 ml/min/mg)	Drug Metab Dispos. 2015 Jul;43(7):928-35.
Recombinant UGT1A8	3.7 µM (Km)	35 minutes	Investigation of intestine-specific UGT1A8's metabolic role, showing highest intrinsic clearance (72 ml/min/mg)	Drug Metab Dispos. 2015 Jul;43(7):928-35.
Human intestinal microsomes (HIM)	1.7 µM (Km)	35 minutes	Evaluation of intestinal UGTs' contribution to OTS167 metabolism, observing OTS167-G formation	Drug Metab Dispos. 2015 Jul;43(7):928-35.
R7T1 β-cells	5-40 nM	48 hours	Assess the induction of human β-cell replication by OTS167	Bioorg Med Chem. 2020 Jan 1;28(1):115193.
LNCaP cells	30 nM	48 hours	To evaluate the effect of OTS167 on apoptosis, results showed that OTS167 increased the fraction of apoptotic cells.	EMBO Mol Med. 2018 Mar;10(3):e8274.
NCI-H295R	1.25 nM, 2.5 nM	48-72 hours	To evaluate the effect of OTS167 on NCI-H295R cell proliferation, results showed that OTS167 had antiproliferative and cytotoxic effects at nanomolar concentrations.	J Exp Clin Cancer Res. 2022 Sep 23;41(1):282.
PBMC	10 nM	6 days	Evaluate the effect of OTS167 on PBMC cells, results showed OTS167 reduced the number of CD138+ cells.	Blood Cancer J. 2016 Aug 19;6(8):e460.
Patient-derived primary ovarian cancer cells	0, 1, 10, 40, 100 nM	72 hours	To evaluate the growth inhibitory effect of OTS167 on patient-derived primary ovarian cancer cells, results showed significant growth inhibition at concentrations of 10, 40, and 100 nM.	J Gynecol Oncol. 2020 Nov;31(6):e93.
Ovarian cancer cell lines (ES-2, OVCAR3, OV-90, PA-1, SKOV-3, SW626, CaOV3, RMG-I, OVSAHO, OVTOKO, A2780)	9.3 to 60 nM (IC50)	72 hours	To evaluate the cytotoxic effect of OTS167 on ovarian cancer cell lines, results showed significant growth inhibition in all tested cell lines.	J Gynecol Oncol. 2020 Nov;31(6):e93.
KMS-34 CFZ	12.25 nM (IC50)	72 hours	Evaluate the effect of OTS167 on KMS-34 CFZ cell viability, results showed OTS167 had comparable effects on the resistant subclone KMS-34 CFZ.	Blood Cancer J. 2016 Aug 19;6(8):e460.
8226 Dox40	60.59 nM (IC50)	72 hours	Evaluate the effect of OTS167 on 8226 Dox40 cell viability, results showed 8226 Dox40 cells were the most resistant to OTS167.	Blood Cancer J. 2016 Aug 19;6(8):e460.
MM1R	12.08 nM (IC50)	72 hours	Evaluate the effect of OTS167 on MM1R cell viability, results showed OTS167 had comparable effects on the resistant subclone MM1R.	Blood Cancer J. 2016 Aug 19;6(8):e460.
U266	18.47 nM (IC50)	72 hours	Evaluate the effect of OTS167 on U266 cell viability, results showed OTS167 decreased cell viability in a dose-dependent manner.	Blood Cancer J. 2016 Aug 19;6(8):e460.
MM1S	7.911 nM (IC50)	72 hours	Evaluate the effect of OTS167 on MM1S cell viability, results showed OTS167 decreased cell viability in a dose-dependent manner.	Blood Cancer J. 2016 Aug 19;6(8):e460.
SK-N-DZ	0 to 1 µM	72 hours	To evaluate the effects of OTS167 on neuroblastoma cell proliferation. Results showed that OTS167 inhibited the proliferation of neuroblastoma cells with high MELK expression at low nM concentrations.	Mol Cancer Ther. 2019 Mar;18(3):507-516.
Ovarian cancer cell lines (ES-2, OVCAR3, OV-90, PA-1, SKOV-3, SW626, CaOV3, RMG-I, OVSAHO, OVTOKO, A2780)	9.3 to 60 nM	72 hours	To evaluate the cytotoxic effect of OTS167 on ovarian cancer cell lines, results showed significant growth inhibition in all tested cell lines.	J Gynecol Oncol. 2020 Nov;31(6):e93.
MOLM:14	50 nM	8-24 hours	OTS167 downregulates FLT3 protein expression in FLT3 mutant AML cell lines.	Blood Cancer J. 2021 Mar 3;11(3):48.
MV4:11	50 nM	8-24 hours	OTS167 downregulates FLT3 protein expression in FLT3 mutant AML cell lines.	Blood Cancer J. 2021 Mar 3;11(3):48.

Nuþ /Nuþ mice	Human ACC xenograft model	Intraperitoneal injection	10 mg/kg	Daily (Monday-Friday) for five weeks	To evaluate the antitumor efficacy of OTS167 in mice with human ACC xenografts, results showed that OTS167 significantly reduced tumor burden.	J Exp Clin Cancer Res. 2022 Sep 23;41(1):282.
NOD scid gamma mice	C4-2b xenograft model	Intraperitoneal injection	10 mg/kg	Daily, for the duration of the experiment	To evaluate the inhibitory effect of OTS167 on tumor growth, results showed that OTS167 significantly reduced tumor growth and induced apoptosis of tumor cells.	EMBO Mol Med. 2018 Mar;10(3):e8274.
Female nude mice	Neuroblastoma xenograft model	Intraperitoneal injection	10 mg/kg	Twice per week for 3 weeks	To evaluate the inhibitory effects of OTS167 on neuroblastoma xenograft growth. Results showed that OTS167 significantly inhibited tumor growth, with the average tumor weight in the treatment group being 77% lower than that in the control group.	Mol Cancer Ther. 2019 Mar;18(3):507-516.
NOD.Cg PrkdcscidIL2rgtm1Wjl/SzJ (NSG) mice	FLT3 mutant AML xenograft mouse model	Oral gavage	5-10 mg/kg	5 days treated/2 days off schedule	OTS167 in combination with TKIs results in prolonged survival in a FLT3 mutant AML xenograft mouse model.	Blood Cancer J. 2021 Mar 3;11(3):48.

Precautionary Statements-General
Code	Phrase
P101	If medical advice is needed,have product container or label at hand.
P102	Keep out of reach of children.
P103	Read label before use
Prevention
Code	Phrase
P201	Obtain special instructions before use.
P202	Do not handle until all safety precautions have been read and understood.
P210	Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
P211	Do not spray on an open flame or other ignition source.
P220	Keep/Store away from clothing/combustible materials.
P221	Take any precaution to avoid mixing with combustibles
P222	Do not allow contact with air.
P223	Keep away from any possible contact with water, because of violent reaction and possible flash fire.
P230	Keep wetted
P231	Handle under inert gas.
P232	Protect from moisture.
P233	Keep container tightly closed.
P234	Keep only in original container.
P235	Keep cool
P240	Ground/bond container and receiving equipment.
P241	Use explosion-proof electrical/ventilating/lighting/equipment.
P242	Use only non-sparking tools.
P243	Take precautionary measures against static discharge.
P244	Keep reduction valves free from grease and oil.
P250	Do not subject to grinding/shock/friction.
P251	Pressurized container: Do not pierce or burn, even after use.
P260	Do not breathe dust/fume/gas/mist/vapours/spray.
P261	Avoid breathing dust/fume/gas/mist/vapours/spray.
P262	Do not get in eyes, on skin, or on clothing.
P263	Avoid contact during pregnancy/while nursing.
P264	Wash hands thoroughly after handling.
P265	Wash skin thouroughly after handling.
P270	Do not eat, drink or smoke when using this product.
P271	Use only outdoors or in a well-ventilated area.
P272	Contaminated work clothing should not be allowed out of the workplace.
P273	Avoid release to the environment.
P280	Wear protective gloves/protective clothing/eye protection/face protection.
P281	Use personal protective equipment as required.
P282	Wear cold insulating gloves/face shield/eye protection.
P283	Wear fire/flame resistant/retardant clothing.
P284	Wear respiratory protection.
P285	In case of inadequate ventilation wear respiratory protection.
P231 + P232	Handle under inert gas. Protect from moisture.
P235 + P410	Keep cool. Protect from sunlight.
Response
Code	Phrase
P301	IF SWALLOWED:
P304	IF INHALED:
P305	IF IN EYES:
P306	IF ON CLOTHING:
P307	IF exposed:
P308	IF exposed or concerned:
P309	IF exposed or if you feel unwell:
P310	Immediately call a POISON CENTER or doctor/physician.
P311	Call a POISON CENTER or doctor/physician.
P312	Call a POISON CENTER or doctor/physician if you feel unwell.
P313	Get medical advice/attention.
P314	Get medical advice/attention if you feel unwell.
P315	Get immediate medical advice/attention.
P320
P302 + P352	IF ON SKIN: wash with plenty of soap and water.
P321
P322
P330	Rinse mouth.
P331	Do NOT induce vomiting.
P332	IF SKIN irritation occurs:
P333	If skin irritation or rash occurs:
P334	Immerse in cool water/wrap n wet bandages.
P335	Brush off loose particles from skin.
P336	Thaw frosted parts with lukewarm water. Do not rub affected area.
P337	If eye irritation persists:
P338	Remove contact lenses, if present and easy to do. Continue rinsing.
P340	Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P341	If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P342	If experiencing respiratory symptoms:
P350	Gently wash with plenty of soap and water.
P351	Rinse cautiously with water for several minutes.
P352	Wash with plenty of soap and water.
P353	Rinse skin with water/shower.
P360	Rinse immediately contaminated clothing and skin with plenty of water before removing clothes.
P361	Remove/Take off immediately all contaminated clothing.
P362	Take off contaminated clothing and wash before reuse.
P363	Wash contaminated clothing before reuse.
P370	In case of fire:
P371	In case of major fire and large quantities:
P372	Explosion risk in case of fire.
P373	DO NOT fight fire when fire reaches explosives.
P374	Fight fire with normal precautions from a reasonable distance.
P376	Stop leak if safe to do so. Oxidising gases (section 2.4) 1
P377	Leaking gas fire: Do not extinguish, unless leak can be stopped safely.
P378
P380	Evacuate area.
P381	Eliminate all ignition sources if safe to do so.
P390	Absorb spillage to prevent material damage.
P391	Collect spillage. Hazardous to the aquatic environment
P301 + P310	IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P301 + P312	IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
P301 + P330 + P331	IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
P302 + P334	IF ON SKIN: Immerse in cool water/wrap in wet bandages.
P302 + P350	IF ON SKIN: Gently wash with plenty of soap and water.
P303 + P361 + P353	IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P304 + P312	IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell.
P304 + P340	IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P304 + P341	IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338	IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P306 + P360	IF ON CLOTHING: Rinse Immediately contaminated CLOTHING and SKIN with plenty of water before removing clothes.
P307 + P311	IF exposed: call a POISON CENTER or doctor/physician.
P308 + P313	IF exposed or concerned: Get medical advice/attention.
P309 + P311	IF exposed or if you feel unwell: call a POISON CENTER or doctor/physician.
P332 + P313	IF SKIN irritation occurs: Get medical advice/attention.
P333 + P313	IF SKIN irritation or rash occurs: Get medical advice/attention.
P335 + P334	Brush off loose particles from skin. Immerse in cool water/wrap in wet bandages.
P337 + P313	IF eye irritation persists: Get medical advice/attention.
P342 + P311	IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P370 + P376	In case of fire: Stop leak if safe to Do so.
P370 + P378	In case of fire:
P370 + P380	In case of fire: Evacuate area.
P370 + P380 + P375	In case of fire: Evacuate area. Fight fire remotely due to the risk of explosion.
P371 + P380 + P375	In case of major fire and large quantities: Evacuate area. Fight fire remotely due to the risk of explosion.
Storage
Code	Phrase
P401
P402	Store in a dry place.
P403	Store in a well-ventilated place.
P404	Store in a closed container.
P405	Store locked up.
P406	Store in corrosive resistant/ container with a resistant inner liner.
P407	Maintain air gap between stacks/pallets.
P410	Protect from sunlight.
P411
P412	Do not expose to temperatures exceeding 50 oC/ 122 oF.
P413
P420	Store away from other materials.
P422
P402 + P404	Store in a dry place. Store in a closed container.
P403 + P233	Store in a well-ventilated place. Keep container tightly closed.
P403 + P235	Store in a well-ventilated place. Keep cool.
P410 + P403	Protect from sunlight. Store in a well-ventilated place.
P410 + P412	Protect from sunlight. Do not expose to temperatures exceeding 50 oC/122oF.
P411 + P235	Keep cool.
Disposal
Code	Phrase
P501	Dispose of contents/container to ...
P502	Refer to manufacturer/supplier for information on recovery/recycling

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CAS No.: 1431697-89-0

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CAS No.: 1431697-89-0

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Physical hazards
Code	Phrase
H200	Unstable explosive
H201	Explosive; mass explosion hazard
H202	Explosive; severe projection hazard
H203	Explosive; fire, blast or projection hazard
H204	Fire or projection hazard
H205	May mass explode in fire
H220	Extremely flammable gas
H221	Flammable gas
H222	Extremely flammable aerosol
H223	Flammable aerosol
H224	Extremely flammable liquid and vapour
H225	Highly flammable liquid and vapour
H226	Flammable liquid and vapour
H227	Combustible liquid
H228	Flammable solid
H229	Pressurized container: may burst if heated
H230	May react explosively even in the absence of air
H231	May react explosively even in the absence of air at elevated pressure and/or temperature
H240	Heating may cause an explosion
H241	Heating may cause a fire or explosion
H242	Heating may cause a fire
H250	Catches fire spontaneously if exposed to air
H251	Self-heating; may catch fire
H252	Self-heating in large quantities; may catch fire
H260	In contact with water releases flammable gases which may ignite spontaneously
H261	In contact with water releases flammable gas
H270	May cause or intensify fire; oxidizer
H271	May cause fire or explosion; strong oxidizer
H272	May intensify fire; oxidizer
H280	Contains gas under pressure; may explode if heated
H281	Contains refrigerated gas; may cause cryogenic burns or injury
H290	May be corrosive to metals
Health hazards
Code	Phrase
H300	Fatal if swallowed
H301	Toxic if swallowed
H302	Harmful if swallowed
H303	May be harmful if swallowed