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Type HazMat fee for 500 gram (Estimated)
Excepted Quantity USD 0.00
Limited Quantity USD 15-60
Inaccessible (Haz class 6.1), Domestic USD 80+
Inaccessible (Haz class 6.1), International USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic USD 100+
Accessible (Haz class 3, 4, 5 or 8), International USD 200+
Chemical Structure| 1222780-33-7 Chemical Structure| 1222780-33-7

Structure of TPPU
CAS No.: 1222780-33-7

Chemical Structure| 1222780-33-7

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TTPU is an inhibitor of epoxide hydrolase (sEH) with IC50 of 3.7 nM with anti-inflammatory and anti-atherosclerotic activities.

4.5 *For Research Use Only !

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Product Details of TPPU

CAS No. :1222780-33-7
Formula : C16H20F3N3O3
M.W : 359.34
SMILES Code : O=C(NC1=CC=C(OC(F)(F)F)C=C1)NC2CCN(C(CC)=O)CC2
MDL No. :MFCD26142949
InChI Key :AAJMQTLFRTZCJK-UHFFFAOYSA-N
Pubchem ID :44142782

Safety of TPPU

GHS Pictogram:
Signal Word:Danger
Hazard Statements:H301
Precautionary Statements:P301+P310
Class:6.1
UN#:2811
Packing Group:

Isoform Comparison

Biological Activity

In Vitro:

Cell Line
Concentration Treated Time Description References
Beas-2B cells 10 µM 24 hours TPPU reduced TGF-β1-induced fibronectin expression Exp Mol Med. 2021 May;53(5):864-874
A549 cells 20 µM 48 hours TPPU reduced the number of BLM-induced β-gal positive cells. Redox Biol. 2023 Jul;63:102765
Mixed mouse glial cultures 10 µM 30 minutes To evaluate the effect of TPPU on mixed glial inflammation, results showed that TPPU suppressed microglial reactivity through astrocytes. Sci Transl Med. 2020 Dec 9;12(573):eabb1206
Mouse primary microglia 10 µM 30 minutes To evaluate the effect of TPPU on microglial inflammation, results showed that TPPU had no direct effect on microglia. Sci Transl Med. 2020 Dec 9;12(573):eabb1206
Mouse primary astrocytes 0.5 µM to 10 µM 30 minutes To evaluate the effect of TPPU on LPS-induced inflammation, results showed that TPPU dose-dependently reduced nitrite release and pro-inflammatory molecule expression. Sci Transl Med. 2020 Dec 9;12(573):eabb1206
MLE12 cells 20 µM 48 hours TPPU significantly reduced the number of BLM-induced β-gal positive cells and reduced the expression of senescence-related p53 and p21 proteins in MLE12 cells. Redox Biol. 2023 Jul;63:102765

In Vivo:

Species
Animal Model
Administration Dosage Frequency Description References
C57BL/6J mice Bleomycin-induced pulmonary fibrosis model Intraperitoneal injection 0.25 mg/kg 5 days per week for 3 weeks TPPU significantly attenuated bleomycin-induced pulmonary fibrosis and reduced collagen levels Exp Mol Med. 2021 May;53(5):864-874
Mice Inflammation and social defeat stress models Oral 0.3, 1.0, or 3.0 mg/kg Single administration or continuous administration for 3 weeks TPPU showed rapid antidepressant effects in both inflammation and social defeat stress models, preventing and alleviating depression-like behaviors. Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):E1944-52
Mice MPTP-induced neurotoxicity model Oral 0.3, 1.0, or 3.0 mg/kg Twice daily from day 2 to day 7 TPPU significantly attenuated MPTP-induced dopaminergic neurotoxicity in the striatum and substantia nigra Proc Natl Acad Sci U S A. 2018 Jun 19;115(25):E5815-E5823
C57BL/6J mice D-galactose-induced premature aging mouse model Intraperitoneal injection 1 mg/kg Once daily for six weeks TPPU significantly inhibited the protein expression of p16, p21, and γH2AX in D-gal-induced premature aging mice and reduced the degree of age-related pulmonary fibrosis. Redox Biol. 2023 Jul;63:102765
Male C57BL/6N mice Scald injury model Intraperitoneal injection 10 mg/kg Three administrations at 48 h, 24 h, and directly before burn injury To evaluate the effect of TPPU on DiHOME levels and immune cell necrosis in scalded mice, it was found that TPPU significantly reduced 12,13-DiHOME levels and decreased necrosis of innate and adaptive immune cells in a dose-dependent manner. Proc Natl Acad Sci U S A. 2022 Mar 29;119(13):e2120691119
Mice Maternal immune activation (MIA) model Drinking water 15 mg/L From P28 to P56 TPPU treatment prevented cognitive deficits and the reduction of PV and GAD67 immunoreactivity in the medial prefrontal cortex (mPFC) of adult offspring after MIA. Proc Natl Acad Sci U S A. 2019 Apr 2;116(14):7083-7088
C57BL/6J mice Myocardial infarction model Oral (via drinking water) 15 mg/L 3 weeks To evaluate the effect of TPPU on cardiac function and fibrosis post-myocardial infarction, results showed that TPPU significantly improved cardiac function and reduced cardiac fibrosis. Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5618-23
C57BL/6 mice PM2.5-mediated lung injury model Intragastric administration 3 mg/kg Three times per week for three weeks To evaluate the protective effect of TPPU on PM2.5-mediated lung injury. Results showed that TPPU treatment reversed PM2.5-induced pulmonary hyperemia, edema, alveolar wall thickening, and macrophage activation, reduced levels of TNF-α, IL-6, MPO, and LDH, and alleviated inflammatory responses by inhibiting the MAPK/NF-κB signaling pathway. J Hazard Mater. 2023 Sep 15;458:131890
Mice 5xFAD transgenic mice Oral gavage or drinking water 3 mg/kg 24 hours or 2.5 to 4.5 months To evaluate the effect of TPPU on acute inflammation and long-term AD pathology, results showed that TPPU reduced neuroinflammation, improved Aβ pathology, and cognitive function. Sci Transl Med. 2020 Dec 9;12(573):eabb1206
Mice Destabilization of the medial meniscus (DMM) model Intraperitoneal injection 3mg/kg Single injection, measured at 3 hours post-injection Acute TPPU injection significantly reversed DMM-induced pain behaviors, including increased weight-bearing on the ipsilateral hind-limb and reversal of the lowered ipsilateral hind-paw withdrawal thresholds. Arthritis Rheumatol. 2022 Apr;74(4):623-633

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

2.78mL

0.56mL

0.28mL

13.91mL

2.78mL

1.39mL

27.83mL

5.57mL

2.78mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2

References

 

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