Cardiac Transcription Factors (CTFs) are key regulatory factors in heart development and function, including GATA4, NKX2-5, and TBX5, among others. These proteins control the expression of genes crucial for the specification, differentiation, and morphogenesis of cardiac cell lineages by binding to DNA. CTFs are primarily located in the nuclei of cardiac cells, coordinating transcriptional networks essential for heart formation and repair. Mutations in the genes encoding CTFs can lead to congenital heart defects and cardiomyopathies, highlighting their indispensable role in cardiac health. Their expression is tightly regulated, displaying specific patterns during different stages of heart development and in response to cardiac stress, indicating the complex regulation of cardiac physiology and pathology.