Enteropeptidase is a crucial digestive enzyme specifically responsible for activating trypsinogen into trypsin, thereby triggering the activation of a cascade of digestive enzymes. It is a heterodimer consisting of heavy and light chains, mainly expressed in the epithelial cells of the small intestine mucosa. By cleaving a specific site on trypsinogen, enteropeptidase initiates a key step in protein digestion. This process is essential for the digestion and absorption of proteins from food. The gene expression of enteropeptidase is tightly regulated, and a deficiency or abnormality in its activity can lead to malabsorption or digestive disorders. The specifics of its expression and mutations are not entirely clear, but they have a potential link to the development of digestive system diseases.