Haspin kinase is a serine/threonine protein kinase primarily localized in the cell nucleus, particularly tightly associated with chromatin during mitosis. It regulates chromosome condensation and proper centromere positioning by specifically phosphorylating serine 3 of histone H3, ensuring the correct separation of sister chromatids. The activity and expression of Haspin kinase are precisely controlled by the cell cycle regulatory mechanisms, and abnormalities or gene mutations in its function are associated with chromosomal instability and tumorigenesis. Therefore, Haspin kinase is a crucial factor in maintaining genome stability and proper mitotic progression, serving as a potential target for cancer therapy.