The Lipoxygenase (LOX) family comprises various enzymes, such as 5-LOX, 12-LOX, and 15-LOX, primarily located in the cytoplasm and nuclear membrane. They catalyze the oxidation of fatty acids to generate biologically active lipid mediators, such as leukotrienes and lipoxins, involved in processes like inflammation, cell proliferation, and apoptosis. LOX exhibits tissue-specific expression in various cells and tissues, regulating immune responses and inflammatory signaling pathways. Dysregulation and mutations in LOX genes are associated with various diseases, including asthma, cancer, and cardiovascular diseases. Studying the activity and regulatory mechanisms of LOX can aid in the development of novel therapeutic approaches.