PKMYT1 (Protein Kinase MYT1) belongs to the tyrosine/threonine protein kinase family, featuring a kinase domain. PKMYT1 is primarily located in the nucleus and regulates the activity of the cell cycle protein kinase Cdc2 through phosphorylation, participating in the regulation of the cell cycle, particularly in the G2/M phase before mitosis. The expression pattern of PKMYT1 varies in different tissues, and mutations or abnormal expression may lead to cell cycle abnormalities, thereby contributing to various diseases such as cancer.