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Chemical Structure| 1315323-00-2 Chemical Structure| 1315323-00-2

Structure of Pyr10
CAS No.: 1315323-00-2

Chemical Structure| 1315323-00-2

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Pyr 10 is a TRPC3-selective inhibitor. IC50 of Ca2+ influx inhibition by Pyr10 in carbachol-stimulated YFP-TRPC3-transfected HEK293 cells for ROCE and thapsigargin-depleted native RBL-2H3 cells for SOCE is 0.72 uM and 13.08 uM.

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Product Details of Pyr10

CAS No. :1315323-00-2
Formula : C18H13F6N3O2S
M.W : 449.37
SMILES Code : O=S(C1=CC=C(C)C=C1)(NC2=CC=C(N3N=C(C(F)(F)F)C=C3C(F)(F)F)C=C2)=O
MDL No. :MFCD28411625
InChI Key :CVALMMCIOLXHDM-UHFFFAOYSA-N
Pubchem ID :53475435

Safety of Pyr10

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H319
Precautionary Statements:P301+P312+P330-P305+P351+P338

Isoform Comparison

Biological Activity

Description
Pyr10, a pyrazole derivative, is a selective inhibitor of the Transient Receptor Potential Cation Channel, Subfamily C, Member 3 (TRPC3). It inhibits calcium influx in TRPC3-transfected HEK293 cells stimulated by carbachol with an IC50 of 0.72 μM, while its IC50 for inhibiting store-operated calcium entry in BRL-2H3 cells is 13.08 μM. This specificity allows Pyr10 to differentiate between receptor-operated TRPC3 channels and native STIM1/Orai1 channels[1].

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

2.23mL

0.45mL

0.22mL

11.13mL

2.23mL

1.11mL

22.25mL

4.45mL

2.23mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2

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