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Type HazMat fee for 500 gram (Estimated)
Excepted Quantity USD 0.00
Limited Quantity USD 15-60
Inaccessible (Haz class 6.1), Domestic USD 80+
Inaccessible (Haz class 6.1), International USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic USD 100+
Accessible (Haz class 3, 4, 5 or 8), International USD 200+
Chemical Structure| 330676-02-3 Chemical Structure| 330676-02-3

Structure of KH7
CAS No.: 330676-02-3

Chemical Structure| 330676-02-3

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KH7 is a specific inhibitor of soluble adenylyl cyclase (sAC), with an IC50 of 3-10 μM against recombinant sACt protein (human and others) in cells. KH7 is also an inhibitor of cAMP.

Synonyms: KH 7 free

4.5 *For Research Use Only !

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Product Details of KH7

CAS No. :330676-02-3
Formula : C17H15BrN4O2S
M.W : 419.30
SMILES Code : OC(C=CC(Br)=C2)=C2/C=N/NC(C(C)SC1=NC(C=CC=C3)=C3N1)=O
Synonyms :
KH 7 free
MDL No. :MFCD01230899

Safety of KH7

GHS Pictogram:
Signal Word:Danger
Hazard Statements:H301
Precautionary Statements:P301+P310
Class:6.1
UN#:2811
Packing Group:

Related Pathways of KH7

GPCR

Isoform Comparison

Biological Activity

In Vitro:

Cell Line
Concentration Treated Time Description References
Islet β-cells 30 μmol/L KH7 inhibited glucose-induced [cAMP]pm oscillations PMC3292328
PBMCs 10 or 25 μM 30 minutes Inhibition of sAC activity, reduction in cAMP levels PMC3072460
PBMCs 25 μM 30 minutes Minimal effect on basal cAMP levels PMC3072460
NHBE cells 50 μM To test the inhibitory effect of KH7 on sAC activity PMC4291545
HEK293T cells 50 μM 48 hours To test the inhibitory effect of KH7 on sAC activity PMC4291545
Primary mouse cholangiocytes 100 μM 3 hours KH7 treatment also prevented NaGCDC-induced apoptosis in primary mouse cholangiocytes. PMC5111777
H69 human cholangiocytes 50-100 μM 1 hour KH7 dose-dependently inhibited BSIA in both control and AE2 knockdown cholangiocytes and at higher concentrations also prevented NaCDC-induced apoptosis altogether. PMC5111777
MIN6 β-cells 30 μmol/L KH7 inhibited glucose-induced [cAMP]pm oscillations PMC3292328
HEK293 cells 50 μM KH7 inhibited the pH recovery rate in sAC-overexpressing HEK293 cells PMC3888009
Cardiac fibers 5 μM 30 minutes KH7, a soluble adenylyl cyclase inhibitor, reduced ADP-stimulated respiratory rates PMC5187875
Nonpigmented ciliary epithelial cells (NPE) 50 μM 2 minutes KH7 significantly reduced the acetazolamide-induced increase in cAMP PMC3888009
MLTC-1 cells 6.25, 12.5, 50, 100 µM 1 hour KH7 dose-dependently inhibited LH-stimulated cAMP accumulation, with 100 µM concentration almost completely abolishing cAMP accumulation PMC8125623
Ciona intestinalis sperm 10 μM 3 min Investigate the effect of KH-7 on sperm motility and trajectory, results showed KH-7 significantly reduced swimming velocity and decreased the diameter of sperm trajectory. PMC4159788
Human endometrial epithelial cell line (ISK) 10 µM 24 hours KH-7, a selective inhibitor of soluble adenylyl cyclase (sAC), was used to investigate the effect of ENaC knockdown or Ca2+ chelation on CFTR upregulation. Results showed that KH-7 completely reversed the CFTR mRNA increase induced by low-degree ENaC knockdown or BAPTA-AM, suggesting the involvement of sAC-driven cAMP production in the regulation of CFTR. PMC9469783

In Vivo:

Species
Animal Model
Administration Dosage Frequency Description References
Mice SAC C2KO mice Perfusion 25 μM To test the regulatory effect of KH7 on CBF PMC4291545
Male DBA/1 J mice Collagen-induced arthritis (CIA) mouse model Intraperitoneal injection 6 mg/kg Three times a week for 6 weeks To evaluate the inhibitory effect of KH7-33 mAb on arthritic symptoms. Results showed that KH7-33 mAb significantly ameliorated the arthritis index, squeaking index, and paw volume. PMC6235220

Clinical Trial:

NCT Number Conditions Phases Recruitment Completion Date Locations
NCT00035490 Arrhythmia Phase 3 Completed - -
NCT03127059 Dysfunctional Breathing in Ast... More >>hma Less << Not Applicable Recruiting November 2020 Denmark ... More >> Dep. of Physiotherapy and Dep. of Pulmonology Recruiting Silkeborg, Central Denmark Region, Denmark, 8600 Contact: Karen H Andreasson       khad@regionsjaelland.dk    Dep. of Physiotherapy and Dep. of Pulmonology Recruiting Bispebjerg, Danish Capital Region, Denmark Contact: Karen H Andreasson       khad@regionsjaelland.dk    Allergi og Lungeklinikken Helsingør Recruiting Helsingør, Danish Capital Region, Denmark, 3000 Contact: Karen H Andreasson       khad@regionsjaelland.dk    Dep. of Physiotherapy and Dep. of Pulmonology Recruiting Hvidovre, Danish Capital Region, Denmark Contact: Karen H Andreasson       khad@regionsjaelland.dk    Dep. of Physiotherapy and Dep. of Pulmonology Recruiting Aalborg, Region North, Denmark Contact: Karen H Andreasson       khad@regionsjaelland.dk    Dep. of Physiotherapy and Dep. of Pulmonology Recruiting Naestved, Region Zealand, Denmark Contact: Karen H Andreasson, PT MSc    +45 24 25 49 26    khad@regionsjaelland.dk    Contact: Uffe Bødtger, MD PhD    +45 56 51 45 92    ubt@regionsjaelland.dk    Dep. of Physiotherapy and Dep. of Pulmonology Recruiting Roskilde, Region Zealand, Denmark Contact: Karen H Andreasson       khad@regionsjaelland.dk Less <<
NCT03616912 Systemic Lupus Erythematosus Phase 3 Recruiting February 16, 2021 -
NCT03518086 Ulcerative Colitis Phase 3 Recruiting December 29, 2021 -
NCT03524092 Ulcerative Colitis Phase 3 Recruiting March 22, 2022 -
NCT02100696 Ulcerative Colitis Phase 3 Recruiting December 26, 2020 -
NCT02136069 Ulcerative Colitis Phase 3 Recruiting March 30, 2023 -
NCT03383146 Gastroparesis ... More >> Diabetes Mellitus Less << Phase 3 Recruiting September 28, 2020 -
NCT01671007 - Active, not recruiting January 14, 2020 -
NCT02129699 Lung Cancer Non-small Cell Sta... More >>ge IV Less << Phase 3 Active, not recruiting September 2020 -
NCT02674308 - Recruiting June 30, 2022 -
NCT03094715 Stroke, Acute ... More >> Cerebral Stroke Cerebrovascular Stroke Apoplexy; Brain Less << Not Applicable Not yet recruiting September 30, 2022 -
NCT02850172 Esophageal Cancer Not Applicable Recruiting April 2019 Taiwan ... More >> National Taiwan University Hospital Recruiting Taipei, Taiwan, 10048 Contact: Yu-Juan Xu    +886-2-23123456 ext 88438    yujuanxu@kimo.com    Contact: Cheryl Chia-Hui Chen    +886-2-33222390    cherylchen@ntu.edu.tw Less <<
NCT02495922 Multiple Myeloma Phase 3 Active, not recruiting March 2021 -
NCT02690831 Manual Therapies ... More >> Respiratory Muscle Training Exercise Therapy Inspiratory Capacity Asthma Less << Not Applicable Completed - Spain ... More >> Centro Superior de Estudios Universitarios La Salle Madrid, Spain, 28023 Less <<
NCT00615940 Metastatic Breast Cancer Phase 2 Completed - United States, New York ... More >> Montefiore Medical Center Weiler Division Department New York, New York, United States, 10461 United States, Ohio Universitys Hospital Case Medical Center Cleveland, Ohio, United States, 44106 United States, Pennsylvania Fox Chase Cancer Center Philadelphia, Pennsylvania, United States, 19111 Belgium AZ Klina, Oncology Department Brasschaat, Belgium, 2930 Institut Jules Bordet Oncologie Médicale Bruxelles, Belgium, 1000 CHU de Liège, Domaine Universitaire de Sart-Tilman, Oncology Department Liège, Belgium, 4000 Brazil Irmandade de Misericórdia da Santa Casa de Porto Alegre Porto Alegre, Brazil, 9005 Instituto Nacional do Câncer - INCA Rio de Janeiro, Brazil, 20560 Instituto Brasileiro de Controle do Câncer - IBCC São Paulo, Brazil, 03102 Germany Gemeinschaftspraxis Dr. Brudler, Dr. Heinrich, Dr. Bangerter Augsburg, Germany, 86150 Klinik und Poliklinik für Frauenheilkunde und Geburtshilfe, Uniklinik Köln Cologne, Germany Universitätsklinikum Essen, Innere Klinik und Poliklinik (Tumorforschung) Essen, Germany, 45147 Uniklinik Frankfurt, Zentrum der Frauenheilkunde und Geburtshilfe Frankfurt/Main, Germany, 60590 Universitätsklinikum der Martin-Luther-Universität Halle-Wittenberg, Poliklinik Gynäkologie Halle/Saale, Germany, 06120 Department of Obstetrics and Gynecology, Technical University Munich, Germany, 81675 Bethesda KH Mönchengladbach, Germany, 41061 Israel Davidof Center, Rabin Medical Center, Department of Oncology Petah Tikva, Israel, 49100 Kaplan Medical Center, Department of Oncolocy Rehovot, Israel, 76100 Sheba Medical Center, Department of Oncology Tel Hashomer, Israel, 52621 Assaf Harofeh medical center, Department of Oncology Zerifin, Israel, 70300 Less <<
NCT02596893 Crohn Disease Phase 3 Terminated(Study terminated by... More >> Sponsor following a recommendation from external DMC based on lack of emerging benefit; there were no new emergent safety findings) Less << - -
NCT01667601 Schizophrenia Phase 2 Phase 3 Completed - China, Jilin ... More >> Jilin Medical College Jilin, Jilin, China, 0432 Hong Kong KH/LKS Specialty OPD Shatin/Mongkok, NT/Kln, Hong Kong TM Psy Centre Tuen Mun, NT, Hong Kong Taiwan Taipei Medical University Taipei, Xinyi, Taiwan Less <<
NCT02283437 Schizophrenia Spectrum Disorde... More >>rs Family Caregivers Less << Not Applicable Active, not recruiting December 31, 2018 Hong Kong ... More >> KH; LKS Specialty OPD Shatin And Mongkok, NT/Kln, Hong Kong Li Ka Shing Specialty OPD Sha Tin, NT, Hong Kong Less <<
NCT01891227 Breast Cancer Phase 2 Completed - Austria ... More >> Hämatologie und Onkologie/Interne E, LKH Feldkirch Feldkirch, Austria, A-6807 Universitätsklinik f. Frauenheilkunde und Geburtshilfe, Klin. Abt. f. Gynäkologie Graz, Austria, 8036 Universitätsklinik f. Innere Medizin, Klin.Abt. f. Onkologie Graz, Austria, 8036 Univ.-Klinik f. Frauenheilkunde; Klinische Abt. f. Gynäkologie u. Geburtshilfe Innsbruck, Austria, A-6020 KH Barmh. Schwestern Linz, Innere Medizin I Hämatologie/Onkologie Linz, Austria, A-4010 Kepler Universitätsklinikum, Med Campus III, Klinik f. Interne 3 - Schwerpunkt Hämatologie u. Onkologie Linz, Austria, A-4021 Universitätsklinik für Innere Medizin III Salzburg, Austria, A-5020 Landeskrankenhaus Steyr, Interne Medizin II Steyr, Austria, A-4400 Less <<
NCT01523639 Colorectal Cancer ... More >> Steatohepatitis Less << Phase 2 Terminated(Prematurely due to ... More >>slow recruitment (07/08/2013). Newly defined study end=LPLV=05/11/2013. ABCSG guaranteed completed treatment period for ethical reasons.) Less << May 2014 Austria ... More >> Medical University Graz, Oncology Graz, Styria, Austria, 8036 Medical University Innsbruck, Internal Medicine Innsbruck, Tyrol, Austria, 6020 Hospital St. Vinzenz Zams, Tyrol, Austria, 6511 Hospital BHS Ried Ried, Upper Austria, Austria, 4910 KH BHB Vienna Vienna, Austria, 1021 Med. Univ. Vienna, General Hospital Vienna Vienna, Austria, 1090 KH St. Josef KH Vienna, Austria, 1130 Less <<
NCT00688259 - Completed - -
NCT00688259 Schizophrenia ... More >> Schizoaffective Disorder Less << Not Applicable Completed - United States, California ... More >> VA Greater Los Angeles Healthcare System, West Los Angeles, CA West Los Angeles, California, United States, 90073 Less <<
NCT02514161 Manual Therapies ... More >> Respiratory Muscle Training Exercise Therapy Inspiratory Capacity Less << Not Applicable Completed - Spain ... More >> Centro Superior de Estudios Universitarios La Salle Madrid, Spain, 28023 Less <<
NCT00975533 Type 2 Diabetes Mellitus Phase 3 Unknown October 2011 China, Hong Kong ... More >> Prince of Wales Hospital Not yet recruiting Shatin, Hong Kong, China Principal Investigator: Simon KH Wong, MBChB          Sub-Investigator: Alice PS Kong, MBChB          Sub-Investigator: Ronald CW Ma, MBChB          Sub-Investigator: Juliana CN Chan, MBBS          Sub-Investigator: Enders KW Ng, MBChB          Sub-Investigator: Vannessa WS Ng, MBChB          Sub-Investigator: Francis CC Chow, MBBS          Sub-Investigator: Wing Yee So, MBChB          Sub-Investigator: Winnie CW Chu, MBChB Less <<
NCT02118584 Ulcerative Colitis Phase 3 Recruiting April 8, 2025 -
NCT00365937 Melanoma Phase 1 Phase 2 Terminated(New regulatories of... More >> the peptides by the pharmaceutical company (the seller)) Less << August 2009 Belgium ... More >> Cliniques Universitaires Saint-Luc Brussels, Belgium, 1200 Less <<
NCT01014026 Uterine Cervical Neoplasms ... More >> Uterine Cervical Dysplasia Less << Phase 3 Completed - France ... More >> IRSA 37 La Riche, France, 37521 IRSA 72 Le Mans, France, 72000 Service d'Orthogénie, CHRU Tours Tours, France, 37000 Service de Gynécologie, CHRU Tours Tours, France, 37000 Association Paul Metadier Tours, France Less <<
NCT03108352 Branch Retinal Vein Occlusion ... More >> Macular Edema Less << Phase 3 Recruiting December 2020 China ... More >> Beijing Friendship Hospital, Capital Medical University Recruiting Beijing, China Contact: Yanling WANG          Peking University People's Hospital Recruiting Beijing, China Contact: Xiaoxin LI          Beijing Tongren Hospital, Capital Medical University Recruiting Peking, China Contact: wenbin WEI          Zhongshan Hospital Recruiting Shanghai, China Contact: Fei YUAN          Tianjin Medical University Eye Hospital Recruiting Tianjing, China Contact: Xiaorong LI          The Eys Hosptial of Wenzhou Medical University Recruiting Wenzhou, China Contact: Xiaoling LIU          The Frist Affiliated Hospital of Xi'an Jiaotong University Recruiting Xi'an, China Contact: Li QIN Less <<
NCT01588301 Cervical Cancer ... More >> Human Papillomavirus Infection Less << Early Phase 1 Completed - -
NCT00741637 Cholera Vibri... More >>o Infections Diarrhea Less << Phase 2 Completed - Thailand ... More >> Department of Preventive and Social Medicine Siriraj Hospital Bangkok, Thailand, 10700 Less <<
NCT02255084 Cancer Cervix Not Applicable Completed - France ... More >> UH Tours Tours, Indre et Loire, France, 37000 Less <<

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

2.38mL

0.48mL

0.24mL

11.92mL

2.38mL

1.19mL

23.85mL

4.77mL

2.38mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2

References

 

Historical Records

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