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Chemical Structure| 7385-67-3 Chemical Structure| 7385-67-3

Structure of Nile Red
CAS No.: 7385-67-3

Chemical Structure| 7385-67-3

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Nile red (Nile blue oxazone) is a lipophilic stain with environment-sensitive fluorescence. It is intensely fluorescent in a lipid-rich environment while having minimal fluorescence in aqueous media. Nile red serves as an excellent vital stain for detecting intracellular lipid droplets by fluorescence microscopy and flow cytometry. It stains intracellular lipid droplets red, with fluorescence wavelengths of 559/635 nm.

Synonyms: Nile Blue A oxazone; Phenoxazone 9

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Product Details of Nile Red

CAS No. :7385-67-3
Formula : C20H18N2O2
M.W : 318.37
SMILES Code : O=C1C2=CC=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=C1
Synonyms :
Nile Blue A oxazone; Phenoxazone 9
MDL No. :MFCD00011639
InChI Key :VOFUROIFQGPCGE-UHFFFAOYSA-N
Pubchem ID :65182

Safety of Nile Red

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P280-P301+P312-P302+P352-P305+P351+P338

Isoform Comparison

Biological Activity

Description
Nile red (Nile blue oxazone) is a lipophilic dye with environment-sensitive fluorescence properties, exhibiting strong fluorescence in lipid-rich environments and minimal fluorescence in aqueous media. It is particularly effective for detecting intracellular lipid droplets via fluorescence microscopy and flow cytometry[1].

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

3.14mL

0.63mL

0.31mL

15.71mL

3.14mL

1.57mL

31.41mL

6.28mL

3.14mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2

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