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With bromine; sodium acetate; In chloroform; at 0 - 20℃; for 1h; |
Step iii & iv: 2-amino-4H-spiro[benzo[dlthiazole-5 J'-cyclopentanl-7(6H)-oneTo a 50 mL round bottom flask, were added <strong>[82683-51-0]spiro[4.5]decane-7,9-dione</strong> (0.65 g, 0.0039 mol), sodium acetate (0.352 g, 0.0043 mol) and chloroform (10 mL). The reaction mixture was cooled to 0 C. To the same flask, bromine (0.624 g, 0.0039 mol) in chloroform (5 mL) was added drop wise. The reaction mixture was stirred at room temperature for 1 h. The reaction mixture was diluted with chloroform and washed with water. The organic layer was separated, washed with brine and dried over anhydrous sodium sulfate. The organic layer was evaporated under reduced pressure to get 8-bromo<strong>[82683-51-0]spiro[4.5]decane-7,9-dione</strong>.This bromo intermediate was stirred with thiourea (0.36 g, 0.0047 mol) in THF (10 mL) at room temperature for 12 h and at reflux temperature for 1 h. The volatiles were evaporated under reduced pressure to get residue. The residue was dissolved in pyridine and stirred at reflux temperature for 2 h. The volatiles were evaporated under reduced pressure to get residue. The residue was partitioned between chloroform and water. The organic layer was separated, washed with brine and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure to get the title compound [0.45 g, 52 %]. LC-MS: 223.1 [M+H]+. |
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With bromine; In acetic acid; for 2h; |
EXAMPLE 1 2-(Mophiholin-4-yl)-4H-spiro[l,3-benzothiazole-5J'-cyclopentan1-7('6H)-oneTo a stirred solution of <strong>[82683-51-0]spiro[4,5]decane-7,9-dione</strong> (1.03 g, 6.2 mmol) in AcOH (10 mL) was added bromine (0.99 g, 0.32 rnL, 6.2 mmol) dropwise. The reaction mixture was stirred for 2 h and then the product was isolated by filtration. The precipitate was washed twice with Et2O (100 mL) and then dried in vacuo. The crude product (0.44 g) was dissolved in TEtaF (50 mL). Intermediate 13 (0.262 g, 1.8 mmol) and N, N- diisopropylethylamine (0.23 g, 0.31 mL, 1.8 mmol) were added. The reaction mixture was heated to 85C for 2 h and then poured into NaHCO3 solution (150 mL) and extracted with EtOAc (150 mL). The organic fraction was dried over MgSO4 and concentrated in vacuo. The crude product was subjected to column chromatography (SiO2, EtOAc) to yield the title compound (0.03 g, 2%, 2 steps) as a white solid, delta? (DMSO-d6) 3.73-3.68 (4H, m), 3.59-3.48 (4H, m), 2.74 (2H, s), 2.42 (2H, s), 1.68-1.57 (4H, m), 1.57-1.38 (4H, m). LCMS (ES+) 293.0 (M+H)+. |