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Chemical Structure| 875756-97-1 Chemical Structure| 875756-97-1

Structure of Hoechst 33342 3HCl
CAS No.: 875756-97-1

Chemical Structure| 875756-97-1

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Hoechst 33342 trihydrochloride is a live nuclear marker dye that binds to DNA grooves, preferentially A/T-rich strands. It enhances fluorescence intensity in A/T-rich DNA and is used for living cell labeling, with fluorescence intensity increasing with pH.

Synonyms: bisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride; NSC 334072

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Product Details of Hoechst 33342 3HCl

CAS No. :875756-97-1
Formula : C27H31Cl3N6O
M.W : 561.93
SMILES Code : [H]Cl.[H]Cl.[H]Cl.CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OCC)C=C6)N5)=C4)N3)=C2)CC1
Synonyms :
bisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride; NSC 334072
MDL No. :MFCD30179380
InChI Key :JABNPSKWVNCGMX-UHFFFAOYSA-N
Pubchem ID :16760503

Safety of Hoechst 33342 3HCl

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H315-H319-H335
Precautionary Statements:P261-P264-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P332+P313

Isoform Comparison

Biological Activity

Description
Hoechst 33342 is a nuclear marker dye in Hoechst series, primarily binding to the A/T-rich regions of DNA strands. Its binding affinity is not restricted to DNA, but the fluorescence intensity is significantly enhanced when bound to A/T-rich double-stranded DNA, making it a valuable tool for live cell labeling. The fluorescence intensity of Hoechst dye increases with the solution's pH, enabling its application across a range of biological research fields[1].

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

1.78mL

0.36mL

0.18mL

8.90mL

1.78mL

0.89mL

17.80mL

3.56mL

1.78mL

Dissolving Methods
The prepared working fluid is recommended to be prepared now and used up as soon as possible in a short period of time. The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1

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