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Chemical Structure| 909089-13-0 Chemical Structure| 909089-13-0

Structure of SKL2001
CAS No.: 909089-13-0

Chemical Structure| 909089-13-0

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SKL2001 is Wnt/β-catenin pathway agonist which interferes in the interaction of Axin/β-catenin.

Synonyms: Wnt Agonist II; SKL2001

4.5 *For Research Use Only !

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Product Citations

Product Citations      Show More

Dai, Xiaochan ; Tang, Zhimin ; Ju, Yahan ; Ni, Ni ; Gao, Huiqin ; Wang, Jiajing , et al.

Abstract: Ametropia is one of the most common ocular disorders worldwide, to which almost half of visual impairments are attributed. Growing evidence has linked the development of ametropia with ambient light, including blue light, which is ubiquitous in our surroundings and has the highest photonic energy among the visible spectrum. However, the underlying mechanism of blue light-mediated ametropia remains controversial and unclear. In the present study, our data demonstrated that exposure of the retinal pigment epithelium (RPE) to blue light elevated the levels of the vital ametropia-related factor type Ⅰ collagen (COL1) via β-catenin inhibition in scleral fibroblasts, leading to axial ametropia (hyperopic shift). Herein, our study provides evidence for the vital role of blue light-induced RPE dysfunction in the process of blue light-mediated ametropia, providing intriguing insights into ametropic aetiology and pathology by proposing a link among blue light, RPE dysfunction and ametropia.

Keywords: Ametropia ; Blue light ; Retinal pigment epithelium ; Scleral fibroblasts ; Type I collagen ; β-catenin

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Zhang, Quan ; Zhang, Cuizhen ; Zhang, Changwen ; Peng, Gang ;

Abstract: Abstract—The nervous system relies upon correct interconnections to exert its normal function. During vertebrate embryonic development, highly stereotyped scaffolds of axon tracts are formed early in the brain to set the foundation for the neuronal interconnections. During zebrafish early development, anterior dorsal telencephalic (ADt) neurons extend axons along the ipsilateral supraoptic tract (SOT) and the contralateral anterior commissure (AC). Our previous study shows axonal outgrowths along the AC/SOT tracts are coordinated by the guidance molecules Dcc-Netrin and Robo2-Slit, but it is not known how the expressions of these guidance molecules are regulated in the forebrain tissues. Here we show ectopic activation of Wnt signaling abolishes the ipsilateral SOT originating from the ADt neurons. Further mechanistic studies show ectopic activation of Wnt signaling significantly reduces Slits’ expression, whilst mis-expression of Slit3 rescues SOT outgrowth. Together, our findings indicate Wnt signaling controls the ipsilateral axonal outgrowth through the regulation of slits’ expression in the zebrafish forebrain.

Keywords: axonal pathfinding ; Wnt signaling ; Slit3 ; forebrain ; zebrafish

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Alternative Products

Product Details of SKL2001

CAS No. :909089-13-0
Formula : C14H14N4O3
M.W : 286.29
SMILES Code : O=C(C1=NOC(C2=CC=CO2)=C1)NCCCN3C=CN=C3
Synonyms :
Wnt Agonist II; SKL2001
MDL No. :MFCD14733081
InChI Key :PQXINDBPUDNMPE-UHFFFAOYSA-N
Pubchem ID :16003447

Safety of SKL2001

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P305+P351+P338

Isoform Comparison

Biological Activity

In Vitro:

Cell Line
Concentration Treated Time Description References
MLO-Y4 cells 60 µM 24 hours Activate Wnt signaling pathway and promote osteoblast differentiation Cells. 2022 Feb 28;11(5):831
cultured blastema cells 40 μM 24 h To test the effect of SKL2001 on Akt phosphorylation, it was found that SKL2001 treatment enhanced Akt phosphorylation. Commun Biol. 2022 Sep 27;5(1):1018.
mouse primary chondrocytes 40 μM 15 h SKL2001, as an agonist of the Wnt/β-catenin pathway, was used to reverse the effect of IL-1β on the Wnt/β-catenin pathway. The results showed that SKL2001 significantly inhibited the cell viability of microsphere-treated IL-1β-stimulated chondrocytes and increased cell apoptosis. Mater Today Bio. 2024 Oct 16;29:101300.
THP-1 macrophages 20 µM 1 h To verify the effect of SKL2001 on the expression of β-catenin and c-Myc in THP-1 macrophages, the results showed that SKL2001 increased the expression of β-catenin and c-Myc. Int J Biol Sci. 2024 Jan 1;20(3):848-863.
CRC cells 30 μM 24 h To investigate the role of β-catenin signaling in the regulation of TFAM expression in CRC cells, 30 μM SKL2001 was added into cell culture medium for 24h. The results showed that SKL2001 significantly upregulated the expression of TFAM. Cancer Commun (Lond). 2021 Aug;41(8):695-714.
Human memory CD8+ T cells 20 μM 7 days SKL2001 mildly suppressed cell proliferation, increased the expression of CD27, CD28, and CD62L, while decreasing the expression of KLRG1, indicating it generates more TCM cells through the Wnt pathway. J Clin Invest. 2022 Jan 18;132(2):e140508.
Human memory CD8+ T cells 20 μM 7 days SKL2001 significantly increased the proportion of IL-2+ cells and enhanced polyfunctionality, indicating it enhances the polyfunctionality of memory CD8+ T cells through the Wnt pathway. J Clin Invest. 2022 Jan 18;132(2):e140508.

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

3.49mL

0.70mL

0.35mL

17.46mL

3.49mL

1.75mL

34.93mL

6.99mL

3.49mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2

References

 

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