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Chemical Structure| 13721-39-6 Chemical Structure| 13721-39-6

Structure of Sodium orthovanadate
CAS No.: 13721-39-6

Chemical Structure| 13721-39-6

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Sodium orthovanadate is an alkaline phosphatase and (Na,K)-ATPase inhibitor with IC50 of 10 μM.

Synonyms: NSC 79534

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Product Citations

Product Citations

Tomasz Kostrzewa ; Jakub Jonczyk ; Joanna Drzeżdżon ; Dagmara Jacewicz ; Magdalena Górska-Ponikowska ; Marcin Kołaczkowski , et al.

Abstract: One of the main goals of recent bioinorganic chemistry studies has been to design and synthesize novel substances to treat human diseases. The promising compounds are metal-based and metal ion binding components such as vanadium-based compounds. The potential anticancer action of vanadium-based compounds is one of area of investigation in this field. In this study, we present five oxovanadium(IV) and dioxovanadium(V) complexes as potential PTP1B inhibitors with anticancer activity against the MCF-7 breast cancer cell line, the triple negative MDA-MB-231 breast cancer cell line, and the human keratinocyte HaCaT cell line. We observed that all tested compounds were effective inhibitors of PTP1B, which correlates with anticancer activity. [VO(dipic)(dmbipy)]·2 H2O (Compound 4) and [VOO(dipic)](2-phepyH)·H2O (Compound 5) possessed the greatest inhibitory effect, with IC50 185.4 ± 9.8 and 167.2 ± 8.0 nM, respectively. To obtain a better understanding of the relationship between the structure of the examined compounds and their activity, we performed a computer simulation of their binding inside the active site of PTP1B. We observed a stronger binding of complexes containing dipicolinic acid with PTP1B. Based on our simulations, we suggested that the studied complexes exert their activity by stabilizing the WPD-loop in an open position and limiting access to the P-loop.

Keywords: protein tyrosine phosphatase PTP1B ; triple negative breast cancer ; oxovanadium(IV) complexes ; dioxovanadium(V) complexes

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Product Details of Sodium orthovanadate

CAS No. :13721-39-6
Formula : Na3O4V
M.W : 183.91
SMILES Code : O=[V]([O-])([O-])[O-].[Na+].[Na+].[Na+]
Synonyms :
NSC 79534
MDL No. :MFCD00003511
InChI Key :IHIXIJGXTJIKRB-UHFFFAOYSA-N
Pubchem ID :61671

Safety of Sodium orthovanadate

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302
Precautionary Statements:P301+P312+P330

Isoform Comparison

Biological Activity

Target
  • ATPase

    (Na,K)-ATPase, IC50:40 nM

In Vitro:

Cell Line
Concentration Treated Time Description References
HepG2-SR cells 5 µM 24 hours SOV significantly reduced intracellular K+ content and decreased ATPase activity. Sci Rep. 2018 Jun 26;8(1):9706.
Huh7-SR cells 10 µM 24 hours SOV significantly reduced the expression of HIF-1α and HIF-2α and inhibited their nuclear translocation. Sci Rep. 2018 Jun 26;8(1):9706.
Leishmania major promastigotes 0–250 µM 48 hours To assess the sensitivity of Leishmania major to phosphatase inhibitors with SPP overexpression or half knockout. The results showed that SPP-overexpressing parasites were more sensitive to the growth inhibition by sodium orthovanadate. Sci Rep. 2022 Sep 26;12(1):16064.

In Vivo:

Species
Animal Model
Administration Dosage Frequency Description References
BALB/c-nu/nu mice Subcutaneous Huh7-SR cell tumor model Intraperitoneal injection 15 mg/kg Once daily for 20 days SOV significantly suppressed tumor growth and enhanced the anti-tumor effect of sorafenib. Sci Rep. 2018 Jun 26;8(1):9706.

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

5.44mL

1.09mL

0.54mL

27.19mL

5.44mL

2.72mL

54.37mL

10.87mL

5.44mL

Dissolving Methods
The prepared working fluid is recommended to be prepared now and used up as soon as possible in a short period of time. The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1

References

 

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