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Chemical Structure| 102036-29-3 Chemical Structure| 102036-29-3

Structure of Protosappanin B
CAS No.: 102036-29-3

Chemical Structure| 102036-29-3

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Protosappanin B, a natural product isolated and purified from the heart wood of Caesalpinia sappan L., significantly increases cell viability, inhibits cell apoptosis and up-regulates the expression of growth-associated protein 43, and induces the degradation of p53 protein, via activation of a MDM2-dependent ubiquitination process.

Synonyms: FT-0689654; Q-100961; (-)-Protosappanin B

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Product Details of Protosappanin B

CAS No. :102036-29-3
Formula : C16H16O6
M.W : 304.29
SMILES Code : OC=1C=CC2=C(OCC(O)(CO)CC3=CC(O)=C(O)C=C23)C1
Synonyms :
FT-0689654; Q-100961; (-)-Protosappanin B
MDL No. :MFCD29048516
InChI Key :QRTYTQTVJQUCEP-UHFFFAOYSA-N
Pubchem ID :13846689

Safety of Protosappanin B

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P305+P351+P338

Related Pathways of Protosappanin B

JAK-STAT

Isoform Comparison

Biological Activity

In Vitro:

Cell Line
Concentration Treated Time Description References
SW620 cells 17.5, 35, 70 µg/ml 24 hours PSB decreased GOLPH3 protein expression in SW620 cells in a dose-dependent manner Integr Cancer Ther. 2020 Jan-Dec;19:1534735420972477
SW620 cells 35 µg/ml 24 hours PSB significantly reduced the migration number of SW620 cells Integr Cancer Ther. 2020 Jan-Dec;19:1534735420972477
SW620 cells 35 µg/ml 24 hours PSB significantly increased the apoptosis rate of SW620 cells Integr Cancer Ther. 2020 Jan-Dec;19:1534735420972477
HCT116 cells 6.25, 12.5, 25, 50, 100, 200 µg/ml 24, 48, 72, 96 hours PSB had poor inhibitory effect on HCT116 cells Integr Cancer Ther. 2020 Jan-Dec;19:1534735420972477
SW620 cells 6.25, 12.5, 25, 50, 100, 200 µg/ml 24, 48, 72, 96 hours PSB inhibited the proliferation of SW620 cells in a dose-dependent and time-dependent manner Integr Cancer Ther. 2020 Jan-Dec;19:1534735420972477
Peripheral blood mononuclear cells (PBMC) 0–120 μg/ml 40 hours NAT exposure enhanced the production of pro-inflammatory TNF and IL-6 by B cells and CD14+ myeloid cells and upregulated the expression of CD40, CD69, and CD95 on B cells. Sci Rep. 2019 Jan 31;9(1):1048
B cells 30 μg/ml 72 hours NAT exposure had no effect on B cell frequency, apoptosis rate, or proliferation. Sci Rep. 2019 Jan 31;9(1):1048

In Vivo:

Species
Animal Model
Administration Dosage Frequency Description References
Balb/c nude mice SW620 cell xenograft model Intraperitoneal injection 20 mg/kg Every 4 days for 27 days PSB distinctly inhibited the tumor growth via suppressing GOLPH3 expression Integr Cancer Ther. 2020 Jan-Dec;19:1534735420972477

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

3.29mL

0.66mL

0.33mL

16.43mL

3.29mL

1.64mL

32.86mL

6.57mL

3.29mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2

References

 

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