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Chemical Structure| 1143-70-0 Chemical Structure| 1143-70-0
Chemical Structure| 1143-70-0

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Urolithin A is a metabolite of ellagitannin with antiproliferative and antioxidant properties. It can cross the blood brain barrier, and may have neuroprotective effects against Alzheimer’s Disease. Urolithin A has been shown to stimulate mitophagy and improve muscle function in aged animals15 and in models of muscular dystrophy,16 while also being safe, bioavailable, and able to induce mitochondrial gene expression in older adults.

Synonyms: 2',7-Dihydroxy-3,4-benzocoumarin; 3,8-Dihydroxy Urolithin

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Heyns, Ingrid Marie ; Ganugula, Raghu ; Kumar, MNV Ravi ; Arora, Meenakshi ;

Abstract: In the past decade, liquid chromatography−mass spectrometry (LC-MS/MS) has become pivotal in clinical diagnosis, drug discovery, and bioanalytical science due to its high sensitivity and rapid analysis. We have developed an ultrasensitive and robust LC-MS/MS method for the simultaneous detection and quantification of cyclosporine A (CsA) and urolithin A (UA) employing ascomycin (ASC) and naringenin (NAR) as internal standards (ISTDs). The method was validated for clinical use, revealing interspecies differences between human plasma and other mammals (e.g., mouse, rat, feline, canine, and bovine serum). Validation parameters, including accuracy, precision, limits of quantification, specificity, selectivity, carryover, linearity, stability, and recovery, met acceptable ICH standards. Linear regression across the full calibration range (1−250 ng/mL for CsA and 0.5−125 ng/mL for UA) yielded an average R2 ≥ 0.999 in all mammal models. The method achieved a limit of quantification (LOQ) of 1−2.5 ng/mL across all model plasma samples with negligible carryover and demonstrated sample stability up to 96 h intra- and interday and 48 h for bovine serum. The method was successfully applied to quantify CsA and UA in canine samples following oral administration from a previous study. With a rapid run time of 6 min, this method offers high selectivity and precision, making it ideal for analyzing limited sample sizes and addressing regulatory challenges. The ability to simultaneously quantify CsA and UA has significant clinical potential for managing complex immuno-inflammatory diseases, enabling precise dose adjustments, and optimizing treatment outcomes.

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Product Details of Urolithin A

CAS No. :1143-70-0
Formula : C13H8O4
M.W : 228.20
SMILES Code : O=C1C2=CC(O)=CC=C2C3=C(O1)C=C(O)C=C3
Synonyms :
2',7-Dihydroxy-3,4-benzocoumarin; 3,8-Dihydroxy Urolithin
MDL No. :MFCD20275235
InChI Key :RIUPLDUFZCXCHM-UHFFFAOYSA-N
Pubchem ID :5488186

Safety of Urolithin A

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302
Precautionary Statements:P280-P305+P351+P338
 

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