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Chemical Structure| 2156-29-8 Chemical Structure| 2156-29-8

Structure of 2-Di-1-ASP
CAS No.: 2156-29-8

Chemical Structure| 2156-29-8

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2-Di-1-ASP is a compound used for mitochondrial staining and fluorescence probing, capable of specifically binding to double-stranded DNA and G-quadruplexes, applicable in cell biology research.

Synonyms: DASPI; 2M2PM; D 308

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Product Details of 2-Di-1-ASP

CAS No. :2156-29-8
Formula : C16H19IN2
M.W : 366.24
SMILES Code : CN(C)C1=CC=C(C=C1)/C=C/C2=CC=CC=[N+]2C.[I-]
Synonyms :
DASPI; 2M2PM; D 308
MDL No. :MFCD00011980
InChI Key :XPOIQAIBZGSIDD-UHFFFAOYSA-M
Pubchem ID :5463010

Safety of 2-Di-1-ASP

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P280-P301+P312-P302+P352-P305+P351+P338

Isoform Comparison

Biological Activity

Description
2-Di-1-ASP (DASPI) functions as a mono-styryl dye, finding extensive application as a fluorescent probe for mitochondria and groove-binding in double-stranded DNA. Specifically, 2-Di-1-ASP demonstrates selectivity towards G-quadruplex (G4) and double-stranded DNA configurations[1].

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

2.73mL

0.55mL

0.27mL

13.65mL

2.73mL

1.37mL

27.30mL

5.46mL

2.73mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2

References

 

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