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Chemical Structure| 21829-25-4 Chemical Structure| 21829-25-4

Structure of Nifedipine
CAS No.: 21829-25-4

Chemical Structure| 21829-25-4

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Nifedipine is a calcium channel blocker with high affinity for L-type calcium channels, with an IC50 value of 10 nM. Nifedipine has antihypertensive and antianginal effects and can be used in cardiovascular disease research.

Synonyms: BAY-a-1040; BAY 1040

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Product Citations

Product Citations

Fofie, Christian Kuete ; Granja-Vazquez, Rafael ; Truong, Vincent ; Walsh, Patrick ; Price, Theodore ; Biswas, Swati , et al.

Abstract: Chronic pain is a global health issue, yet effective treatments remain limited due to poor preclinical-to-human translation. To address this, we developed a high-content screening (HCS) platform using hiPSC-derived nociceptors to identify analgesics targeting the peripheral nervous system. These cells, cultured on multi-well microelectrode arrays, achieved nearly 100% active electrodes by week 2, maintaining stable activity for at least 2 weeks. After 28 days, we assessed drug effects on neuronal activity, achieving strong assay performance (robust Z′ > 0.5). Pharmacological tests confirmed responses to key analgesic targets, including ion channels (Nav, Cav, Kv, and TRPV1), neurotransmitter receptors (AMPAR and GABA-R), and kinase inhibitors (tyrosine and JAK1/2). Transcriptomic analysis validated target expression, though levels differed from primary human DRG cells. The platform was used to screen over 700 natural compounds, demonstrating its potential for analgesic discovery. This HCS platform facilitates the rapid discovery of uncharacterized analgesics, reducing preclinical-to-human translation failure.

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Product Details of Nifedipine

CAS No. :21829-25-4
Formula : C17H18N2O6
M.W : 346.33
SMILES Code : O=C(C1=C(C)NC(C)=C(C(OC)=O)C1C2=CC=CC=C2[N+]([O-])=O)OC
Synonyms :
BAY-a-1040; BAY 1040
MDL No. :MFCD00057326
InChI Key :HYIMSNHJOBLJNT-UHFFFAOYSA-N
Pubchem ID :4485

Safety of Nifedipine

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H351-H361
Precautionary Statements:P280-P305+P351+P338

Isoform Comparison

Biological Activity

Target
  • Calcium Channel

In Vitro:

Cell Line
Concentration Treated Time Description References
Rat aortic smooth muscle cells 25 and 100 nM 30 min To investigate the effect of Nifedipine on Ang II-induced NADPH oxidase activity, results showed that Nifedipine significantly attenuated Ang II-induced NADPH oxidase activity. PMC4637219
Mouse liver S9 fraction 50 μM 40 min To test the activity of mouse hepatic CYP3A11, the results showed that the generation of oxidized nifedipine in control mice was significantly higher than in treated mice PMC8727778
Gastric Smooth Muscle Cells 10 μM Used to test the occurrence of L-type calcium current (ICa,L). PMC7767160
CAFs 2.5 μM 24 h To assess the effect of Nifedipine on the activated state of CAFs, results showed that Nifedipine reduced CAF motility and ECM remodeling ability, and decreased MMP-2 release. PMC11165820
DU145 cells 2.5 μM 24 h To evaluate the effect of Nifedipine-treated CAFs on the growth of DU145 cells, results showed that Nifedipine significantly inhibited the growth of DU145 cells. PMC11165820
LNCaP cells 14.69 µM 72 h Nifedipine showed dose-dependent antiproliferative effects in LNCaP cells with an IC50 value of 14.69 µM. PMC8762999
DU145 cells 26.66 µM 72 h Nifedipine showed dose-dependent antiproliferative effects in DU145 cells with an IC50 value of 26.66 µM. PMC8762999
MGC-803 cells 20.73 µM 72 h Nifedipine showed toxicity in the MGC-803 cell line with an IC50 value of 20.73 µM. PMC8762999
hSSC-derived cells 5 μM 3 weeks To determine the Ca2+ influx in hSSC-derived cells, results showed that nifedipine significantly reduced the Ca2+ influx. PMC6598262

In Vivo:

Species
Animal Model
Administration Dosage Frequency Description References
Mice Cacna1d KO mice Apical administration 10 μM Single dose To investigate the effect of Nifedipine on ileal calcium absorption in Cacna1d KO mice, results showed that Nifedipine significantly inhibited ileal calcium absorption in WT mice but not in Cacna1d KO mice. PMC6889763
Mice Awake and freely moving mouse model Intraperitoneal injection 1 mg/kg Single administration, observed for 6 hours To investigate the modulation of JWH-018-induced cardiovascular and respiratory responses by Nifedipine. Results showed that Nifedipine did not significantly reverse the JWH-018-induced bradycardia but slightly reduced the JWH-018-induced tachyarrhythmic events during the last two hours of the experiment. Additionally, Nifedipine restored the JWH-018-induced reduction in breath rate and SpO2 immediately after administration. PMC10142259
hph-1 mice Ang II-induced abdominal aortic aneurysm model Oral 5 and 20 mg/kg Once daily for 2 weeks To investigate the effect of Nifedipine on AAA formation, results showed that Nifedipine significantly reduced the incidence of AAA and improved eNOS coupling and NO bioavailability. PMC4637219
Mice Chronic colitis model Oral 20 mg/kg Once daily for 14 weeks To study the effects of long-term exposure to RTS and/or DSS on the liver and intestines of mice, the results showed that RTS exposure caused intestinal injury, and DSS exacerbated RTS-induced liver injury PMC8727778
SCID mice Prostate cancer xenograft model Intratumoral injection 250 μl 5 consecutive days for 2 weeks To evaluate the effect of Nifedipine-treated CAFs on the growth of prostate cancer xenografts, results showed that Nifedipine significantly inhibited tumor growth. PMC11165820
Male athymic nude mice Prostate cancer xenograft model Intraperitoneal injection 50 mg/kg Once daily for 18 days Nifedipine significantly inhibited the growth of prostate tumors without causing apparent changes. PMC8762999

Clinical Trial:

NCT Number Conditions Phases Recruitment Completion Date Locations
NCT00000102 Congenital Adrenal Hyperplasia Phase 1 Phase 2 Completed - United States, South Carolina ... More >> Medical University of South Carolina Charleston, South Carolina, United States Less <<
NCT01010048 Urinary Calculus Phase 4 Unknown December 2010 China, Chongqing ... More >> The First Affiliated Hospital, ChongQing medical University Recruiting ChongQing, Chongqing, China, 400016 Contact: Yunfeng He, Doctor    86-23-89011121       Contact: Xiaohou Wu, Doctor    86-23-89011122 Less <<
NCT00280462 Ocular Physiology ... More >> Regional Blood Flow Less << Not Applicable Completed - Austria ... More >> Department of Clinical Pharmacology Vienna, Austria, 1090 Less <<
NCT01912677 Hypertension in Pregnancy ... More >> Preeclampsia Less << Phase 4 Completed - India ... More >> Daga Women's Hospital Nagpur, Maharashtra, India, 440003 Government Medical College Nagpur, Maharashtra, India, 440003 Less <<
NCT02933593 Hypertension Not Applicable Withdrawn(PI withdrew the prot... More >>ocol) Less << - -
NCT00722215 Chronic Kidney Disease ... More >> Proteinuria Less << Phase 1 Completed - United Kingdom ... More >> Clinical Research Centre, Western General Hospital Edinburgh, Scotland, United Kingdom, EH4 2XU Less <<
NCT00817037 Chronic Kidney Disease ... More >> Proteinuria Blood Pressure Less << Phase 2 Unknown - United Kingdom ... More >> Clinical Research Centre, Western General Hospital Edinburgh, Scotland, United Kingdom, EH4 2XU Less <<
NCT02337881 Labor Preterm Requiring Hospit... More >>alization Less << Phase 1 Unknown October 2016 Egypt ... More >> Menoufia University Recruiting Shebin Elkom, Egypt Principal Investigator: tarek sayed          Saudi Arabia Alhayah national hospital Recruiting Abha, Saudi Arabia Principal Investigator: mohamed maher Less <<
NCT00641784 Labor, Premature Phase 3 Terminated March 2012 United States, California ... More >> Memorial Care Center for Women/Long Beach Memorial Medical Center Long Beach, California, United States, 92806 Less <<
NCT01363336 - Completed - Japan ... More >> Many Locations, Japan Less <<
NCT01351428 Pre-Eclampsia ... More >> Pregnancy Hypertension Less << Not Applicable Completed - Canada, Ontario ... More >> Mount Sinai Hospital Toronto, Ontario, Canada, M5G1X5 Less <<
NCT03695107 - Recruiting September 10, 2020 China, Hunan ... More >> The Third Xiangya Hospital of Central South University , , China, Recruiting Changsha, Hunan, China, 410013 Contact: Yang Guoping , professor, doctor    0086 0731 88618326    ygp9880@126.com Less <<
NCT02885558 Endothelium, Vascular Not Applicable Recruiting December 2018 Denmark ... More >> Centre for Phsical Activity Research Not yet recruiting Copenhagen, Denmark, 2100 Contact: Stefan P Mortensen, DMSc       smortensen@health.sdu.dk    Cardiovascular and Renal Research Recruiting Odense, Denmark, 5000 Contact: Stefan P Mortensen, DMSc       smortensen@health.sdu.dk Less <<
NCT01518855 Hypertension Phase 4 Completed - Japan ... More >> Sapporo, Hokkaido, Japan, 060-0003 Setagaya-ku, Tokyo, Japan, 158-0097 Shinjuku-ku, Tokyo, Japan, 163-6003 Hiroshima, Japan, 733-0011 Less <<
NCT00728858 - Completed - -
NCT00519870 Pulmonary Hypertension Phase 3 Completed - -
NCT01215708 Renal Calculi Phase 3 Unknown December 2010 Brazil ... More >> Clinics Hospital of University of Sao Paulo Sao Paulo, Brazil, 05403900 Less <<
NCT01830530 Hypertension ... More >>High Altitude Less << Phase 4 Completed - Peru ... More >> Universidad Peruana Cayetano Heredia Lima, Peru Less <<
NCT02725736 Labor Preterm Multiple Phase 3 Unknown March 2018 -
NCT01348880 Erectile Dysfunction Phase 1 Completed - United States, Florida ... More >> Miami, Florida, United States, 33014 United States, Tennessee Knoxville, Tennessee, United States, 37920 Less <<
NCT03136666 Clinical Pharmacology Phase 1 Completed - Germany ... More >> Wuppertal, Nordrhein-Westfalen, Germany, 42096 Less <<
NCT00391339 Tocolysis Phase 2 Phase 3 Unknown - Switzerland ... More >> Department of Obstetrics, University of Zuerich Not yet recruiting Zuerich, Switzerland, 8091 Principal Investigator: Roland Zimmermann, Prof. Dr. med.          Principal Investigator: Ursula von Mandach, Prof. Dr. pharm. Less <<
NCT00713011 Diabetic Nephropathies ... More >> Hypertension Less << Phase 3 Withdrawn(Study was stopped by... More >> sponsor prior to first patient assignment to groups due to operational reasons.) Less << March 2009 -
NCT00620724 Placenta Previa Phase 2 Completed - France ... More >> University Hospital Rouen Rouen, France, 76031 Less <<
NCT03506724 Hypertension in Pregnancy ... More >> Preeclampsia Less << Phase 4 Recruiting January 2019 United States, New York ... More >> Maimonides Hospital Recruiting Brooklyn, New York, United States, 11219 Contact: Poroshat Shekarloo, MD       pshekarloo@maimonidesmed.org    Mount Sinai West Recruiting New York, New York, United States, 10019 Contact: Dyese Taylor, MD    212-523-7579    dyese.taylor@mountsinai.org    Principal Investigator: Dyese Taylor, MD Less <<
NCT03654378 - Recruiting December 2022 United States, Illinois ... More >> Northwestern University Asher Center for the Study and Treatment of Depressive Disorders Recruiting Chicago, Illinois, United States, 60611 Contact: Minaz K Cattan, M.D.    312-695-7190    minaz.kolia@northwestern.edu    Contact: Gabrielle A Mesches, M.S.    312-695-6684    gabrielle.mesches@northwestern.edu    Principal Investigator: Katherine L Wisner, M.D., M.S. Less <<
NCT01429545 Preterm Labour Phase 2 Completed - United Arab Emirates ... More >> Department of obstetrics and Gynecology, Tawam Hospital Al Ain, Abudhabi, United Arab Emirates, 15258 Tawam Hospital Al Ain, Abudhabi, United Arab Emirates, 15258 Less <<
NCT01796522 Preterm Delivery. Phase 3 Withdrawn(have been published ... More >>new studies that it showed the ineffectiveness of the treatments proposed in this study.) Less << May 2013 Spain ... More >> Hospital Universitari y Politécnic La Fe Valencia, Spain, 46026 Less <<
NCT00750113 Hypertension Phase 4 Completed - Italy ... More >> Pozzilli, Isernia, Italy, 86077 Monza, Monza-Brianza, Italy, 20052 Somma Lombardo, Varese, Italy, 21013 Ancona, Italy, 60126 Bologna, Italy, 40138 Brescia, Italy, 25123 Broni, Italy, 27043 Catania, Italy, 95126 Cinisello Balsamo, Italy, 20092 Ferrara, Italy, 44100 L'Aquila, Italy, 67100 Milano, Italy, 20143 Napoli, Italy, 80136 Napoli, Italy, 80141 Novara, Italy, 28100 Padova, Italy, 35128 Palermo, Italy, 90127 Pavia, Italy, 27100 Perugia, Italy, 06129 Pisa, Italy, 56126 Reggio Emilia, Italy, 42100 Roma, Italy, 00152 Roma, Italy, 00157 Roma, Italy, 00161 Sassari, Italy, 07100 Siena, Italy, 53100 Siracusa, Italy, 96100 Treviso, Italy, 31100 Trieste, Italy, 34149 Varese, Italy, 21100 Venezia, Italy, 30122 Spain Ferrol, A Coruña, Spain, 15405 Gijón, Asturias, Spain, 33394 Badalona, Barcelona, Spain, 08916 Jerez de la Frontera, Cádiz, Spain, 11407 Las Palmas de Gran Canaria, Las Palmas, Spain, 35020 Beniganim, Valencia, Spain, 46830 Badajoz, Spain, 06080 Ciudad Real, Spain, 13005 Madrid, Spain, 28040 Madrid, Spain, 28041 Málaga, Spain, 29010 Valencia, Spain, 46006 Less <<
NCT01118286 - Completed - Bahrain ... More >> Many Locations, Bahrain Egypt Many Locations, Egypt Jordan Many Locations, Jordan Lebanon Many Locations, Lebanon Morocco Many Locations, Morocco Oman Many Locations, Oman Pakistan Many Locations, Pakistan Qatar Many Locations, Qatar Russian Federation Many Locations, Russian Federation Saudi Arabia Many Locations, Saudi Arabia United Arab Emirates Many Locations, United Arab Emirates Less <<
NCT01030081 Hypertension Phase 4 Completed - China ... More >> Shanghai Institite of Hypertension, Ruijin Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China, 200025 Less <<
NCT00173667 Hypertension Phase 4 Unknown - Taiwan ... More >> Cardiovascular Division, Department of Internal Medicine, National Taiwan University Hospital Not yet recruiting Taipei, Taiwan Contact: Gen-Gae Chen, MD    886-2-23123456 Less <<
NCT01355367 Hypertension Phase 3 Completed - Japan ... More >> Hirosaki, Aomori, Japan, 036-8082 Kamagaya, Chiba, Japan, 273-0100 Asahikawa, Hokkaido, Japan, 070-0061 Asahikawa, Hokkaido, Japan, 078-8214 Sapporo, Hokkaido, Japan, 003-0026 Sapporo, Hokkaido, Japan, 003-0825 Sapporo, Hokkaido, Japan, 004-0004 Sapporo, Hokkaido, Japan, 007-0841 Sapporo, Hokkaido, Japan, 062-0053 Sapporo, Hokkaido, Japan, 063-0841 Sapporo, Hokkaido, Japan, 064-0803 Sapporo, Hokkaido, Japan, 064-0807 Kawasaki, Kanagawa, Japan, 210-0852 Daito, Osaka, Japan, 574-0074 Kishiwada, Osaka, Japan, 596-8522 Yao, Osaka, Japan, 581-0011 Tokorozawa, Saitama, Japan, 359-1141 Hachioji, Tokyo, Japan, 192-0046 Meguro, Tokyo, Japan, 152-0031 Minato, Tokyo, Japan, 105-7390 Minato, Tokyo, Japan, 108-0075 Shizuoka, Japan, 421-0193 Less <<
NCT02912910 - Withdrawn(PI withdrew protocol... More >>) Less << - -
NCT00672113 Hypertension Phase 4 Completed - United Kingdom ... More >> Cambridge, Cambridgeshire, United Kingdom, CB2 2XY Less <<
NCT01985594 Preterm Labor Phase 2 Unknown October 2014 Malaysia ... More >> Obstetric and Gynaecology Department, National University of Malaysia Medical Centre Recruiting Cheras, Kuala Lumpur, Malaysia, 56000 Contact: national university of malaysia medical centre    60391455950       Sub-Investigator: nor zila hassan malek          Principal Investigator: nor azlin mohamed ismail Less <<
NCT03449277 Postpartum Preeclampsia Phase 4 Recruiting March 1, 2019 Egypt ... More >> Hossam Ahmed Abd Ellah Recruiting Assiut, Egypt Contact: Hossam Ahmed Abd Ellah, MBBCH    0201119939775    drhaaada@gmail.com Less <<
NCT00244530 Kidney Failure, Chronic ... More >> Coronary Artery Disease Less << Phase 4 Completed - Norway ... More >> Rikshospitalet University Hospital Oslo, Norway, 0027 Less <<
NCT00993109 Hypertension Phase 4 Completed - China, Guangdong ... More >> Guangzhou, Guangdong, China, 510080 China, Hebei Shijiazhuang, Hebei, China, 050051 China, Hunan Changsha, Hunan, China, 410008 Changsha, Hunan, China, 410013 China, Jiangsu Nanjing, Jiangsu, China, 210008 Nanjing, Jiangsu, China, 210029 China, Liaoning Shenyang, Liaoning, China, 110001 China Beijing, China, 100029 Beijing, China, 100037 Shanghai, China, 200025 Korea, Republic of Donggu,, Gwangju Gwang''yeogsi, Korea, Republic of, 501757 Bucheon-si,, Gyeonggido, Korea, Republic of Yangsan-si, Gyeongnam, Korea, Republic of Jongno-gu, Korea, Republic of Jung-gu, Korea, Republic of Seoul, Korea, Republic of, 110-744 Seoul, Korea, Republic of, 120-752 Less <<
NCT02047019 Hypertension Phase 3 Withdrawn(GPDC decided to term... More >>inate the study) Less << December 8, 2019 Italy ... More >> Fondazione Università G.D'Annunzio Chieti, Abruzzo, Italy, 66100 A.O.U. di Bologna Policlinico S.Orsola Malpighi Bologna, Emilia-Romagna, Italy, 40138 AAS 3 Friuli Alto Medio Collin Udine, Friuli-Venezia Giulia, Italy, 33038 Fondazione Salvatore Maugeri Pavia, Lombardia, Italy, 27100 IRCCS Ist Neurologico Mediterraneo Isernia, Molise, Italy, 86077 A.O.U. di Sassari Sassari, Sardegna, Italy, 07100 A.O.U. Pisana Pisa, Toscana, Italy, 56126 AULSS 07 Pieve Soligo Treviso, Veneto, Italy, 31029 Less <<
NCT02513927 Hypertension Phase 2 Phase 3 Unknown October 2018 Hungary ... More >> Twenty-four-hour ambulatory BP monitoring Recruiting Budapest, Hungary Contact: Jingyan Piao, MM    86-451-85555333    411483521@qq.com    Contact: Yujiao Pan, MM    86-451-85555671    panyujiao@163.com Less <<
NCT01071122 Hypertension Phase 4 Completed - Korea, Republic of ... More >> Goyang-si, Gyeonggido, Korea, Republic of, 411-706 Goyang, Gyeonggido, Korea, Republic of, 410-773 Chungchungbuk-do, Korea, Korea, Republic of, 361-711 Seoul, Korea, Korea, Republic of, 135-720 Seoul, Seoul Teugbyeolsi, Korea, Republic of, 152-703 Busan, Korea, Republic of Gwangju, Korea, Republic of, 501-757 Joong-gu, Korea, Republic of, 100-380 Kungki-do, Korea, Republic of, 463-707 Seoul, Korea, Republic of, 110-744 Seoul, Korea, Republic of, 120-752 Seoul, Korea, Republic of, 135-710 Seoul, Korea, Republic of, 136-705 Seoul, Korea, Republic of, 137-701 Suwan, Korea, Republic of, 443-721 Less <<
NCT03542552 Placenta Previa Phase 3 Not yet recruiting January 2020 -
NCT01287260 Hypertension Phase 3 Completed - Japan ... More >> Hirosaki, Aomori, Japan, 036-8082 Kamagaya, Chiba, Japan, 273-0100 Asahikawa, Hokkaido, Japan, 070-0061 Asahikawa, Hokkaido, Japan, 078-8214 Sapporo, Hokkaido, Japan, 003-0026 Sapporo, Hokkaido, Japan, 003-0825 Sapporo, Hokkaido, Japan, 004-0004 Sapporo, Hokkaido, Japan, 007-0841 Sapporo, Hokkaido, Japan, 062-0053 Sapporo, Hokkaido, Japan, 063-0841 Sapporo, Hokkaido, Japan, 064-0803 Sapporo, Hokkaido, Japan, 064-0807 Kawasaki, Kanagawa, Japan, 210-0852 Daito, Osaka, Japan, 574-0074 Kishiwada, Osaka, Japan, 596-8522 Yao, Osaka, Japan, 581-0011 Tokorozawa, Saitama, Japan, 359-1141 Hachioji, Tokyo, Japan, 192-0046 Meguro, Tokyo, Japan, 152-0031 Minato, Tokyo, Japan, 105-7390 Minato, Tokyo, Japan, 108-0075 Shizuoka, Japan, 421-0193 Less <<
NCT00816205 Anal Resting Pressure Phase 1 Phase 2 Completed - Israel ... More >> Dept of Gastroeneterology, Asaf Harofe Medical Center Zrifin, Israel Less <<
NCT00768560 Hypertension Phase 2 Completed - Japan ... More >> Sendai, Miyagi, Japan, 982-0032 Suita, Osaka, Japan, 565-0853 Koshigaya, Saitama, Japan, 343-0827 Less <<
NCT01294215 Hypertension Phase 3 Completed - Japan ... More >> Ebina, Kanagawa, Japan, 243-0401 Sagamihara, Kanagawa, Japan, 252-0311 Kawaguchi, Saitama, Japan, 332-0012 Shinjuku, Tokyo, Japan, 169-0072 Less <<
NCT00599898 Labor, Premature Phase 4 Completed - Israel ... More >> HaEmek Medical Center Afula, Israel, 18100 Less <<
NCT01314859 Threatened Preterm Labor Phase 3 Withdrawn July 2013 -
NCT02563405 Hypertension Phase 2 Phase 3 Unknown October 2018 China, Heilongjiang ... More >> uric acid test and 24-hour ambulatory BP monitoring Recruiting Harbin, Heilongjiang, China, 150001 Contact: Yujiao Pan, MM    86-451-85555671    panyujiao@163.com Less <<
NCT01303783 Hypertension, Essential Phase 2 Completed - -
NCT00185900 - Completed - -
NCT02357615 Essential Hypertension Phase 4 Unknown September 2015 China ... More >> Ruijin Hospital Affiliated to Shanghai Jiao Tong University Medical School Recruiting Shanghai, China Contact: Pingjin Gao Less <<
NCT00712738 Thalassemia I... More >>ron Overload Hemochromatosis Less << Phase 1 Completed - United States, Maryland ... More >> National Institutes of Health Clinical Center, 9000 Rockville Pike Bethesda, Maryland, United States, 20892 Less <<
NCT00185900 Obstetric Labor, Premature Not Applicable Completed - United States, California ... More >> Stanford University School of Medicine Stanford, California, United States, 94305 Less <<
NCT03245307 Cytochrome P450 Interaction Phase 1 Recruiting September 30, 2018 China, Liaoning ... More >> Chinese Medical University First Hospital Recruiting Shenyang, Liaoning, China Contact: Yunpeng Liu    86024-83282802    cmu_trial@163.com Less <<
NCT00768560 - Completed - -
NCT00486824 - Completed - -
NCT00486824 Obstetric Labor, Premature Phase 1 Phase 2 Completed - United States, California ... More >> Stanford University School of Medicine Stanford, California, United States, 94305 Less <<
NCT01586286 Levator Ani Syndrome Not Applicable Withdrawn(No patients were rec... More >>ruited over a 12 month period) Less << May 2014 United States, Tennessee ... More >> Vanderbilt University Medical Center Nashville, Tennessee, United States, 37232 Less <<
NCT01788358 - Completed - -
NCT00306462 Premature Birth ... More >> Premature Labor Less << Not Applicable Terminated(No enrollment in pa... More >>st year, lack of interest) Less << - United States, Ohio ... More >> University Hospital Cincinnati, Ohio, United States, 45219 Less <<
NCT00496184 Vulvar Diseases ... More >> Vulvar Pain Vestibulitis Vestibulodynia Vulvodynia Less << Phase 3 Completed - Israel ... More >> Colposcopy Clinic, Obstetrics and Gynecology Department, Western galilee Hospital Nahariya, Israel Department of Obstetrics and Gynecology Nahariya, Israel Less <<
NCT01346657 Healthy Volunteers Phase 4 Completed - Taiwan ... More >> Taipei Medical University - WanFang Hospital Taipei, Taiwan Less <<
NCT01157481 Diastolic Heart Failure Not Applicable Unknown December 2016 Japan ... More >> Hokkaido Univestity Hospital Sapporo, Japan, 060-8638 Less <<
NCT02583633 Managing Preterm Labor Phase 4 Completed - -
NCT02499822 Hypertension ... More >>High Blood Pressure Variability Less << Phase 4 Recruiting December 2018 China ... More >> Centre for Epidemiological Studies and Clinical Trials, Ruijin Hospital Shanghai Institute of Hypertension, Shanghai Jiaotong University School of Medicine Recruiting Shanghai, China Contact: Jiguang Wang, MD       jiguangwang@aim.com    Greece Hypertension Center, Third University Department of Medicine, Sotiria Hospital Not yet recruiting Athens, Greece Contact: George S Stergiou, MD       gstergi@med.uoa.gr    Italy Istituto Auxologico Italiano Recruiting Milan, Italy Contact: Gianfranco Parati, MD    390261911 ext 2890    gianfranco.parati@unimib.it Less <<
NCT00185952 Obstetric Labor, Premature ... More >> Venous Thrombosis Less << Not Applicable Completed - United States, California ... More >> Stanford University School of Medicine Stanford, California, United States, 94305 Less <<
NCT01788358 Hypertension Phase 3 Completed - -
NCT01435161 Atrial Fibrillation Phase 4 Completed - China, Chongqing ... More >> 2ndChongqingMU Chongqing, Chongqing, China, 400010 Less <<
NCT00137501 Labor, Premature Phase 3 Terminated(The trial was termi... More >>nated because of difficulties in recruitement.) Less << - Lebanon ... More >> American University of Beirut Medical Center Beirut, Lebanon Less <<
NCT01167153 Hypertension Phase 4 Completed - China ... More >> Novartis Pharmaceuticals Beijing, China Less <<
NCT02031861 Essential Hypertension Phase 4 Completed - China, Shanghai ... More >> Ruijin Hospital Affiliated to Shanghai Jiao Tong University Medical School Shanghai, Shanghai, China, 200025 Less <<
NCT02426177 Postnatal Hypertension Phase 3 Unknown December 2016 -
NCT00525486 Preterm Labor Not Applicable Terminated - -
NCT03027674 Raynaud Phenomenon Due to Trau... More >>ma Raynaud Disease System; Sclerosis Lupus Erythematosus, Systemic Dermatomyositis Ultrasound Therapy; Complications Less << Early Phase 1 Completed - Chile ... More >> Department of Dermatology, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile. Santiago, RM, Chile, 7820436 Less <<
NCT01167153 - Completed - -
NCT00865891 Healthy Phase 1 Completed - United States, North Dakota ... More >> PRACS Institute, Ltd. Fargo, North Dakota, United States, 58102 Less <<
NCT01227603 Hypertension, Essential Phase 1 Completed - Germany ... More >> Köln, Nordrhein-Westfalen, Germany, 51063 Less <<
NCT00864617 Healthy Phase 1 Completed - United States, North Dakota ... More >> PRACS Institute, Ltd. Fargo, North Dakota, United States, 58102 Less <<
NCT02168309 Severe Postpartum Hypertension Not Applicable Completed - United States, California ... More >> Cedars Sinai Medical Center Los Angeles, California, United States, 90048 Less <<
NCT03194633 - Recruiting May 31, 2019 China ... More >> Many Locations Recruiting Multiple Locations, China Less <<
NCT02168309 - Completed - -
NCT01360034 Obstetric Labor, Premature Phase 3 Unknown June 2016 Panama ... More >> Saint Thomas Hospital Panama, Panama Less <<
NCT02641821 Hypertension Phase 4 Unknown December 2016 China ... More >> Division of Cardiology, Peking University First Hospital Recruiting Beijing, China, 100034 Contact: Yang Yu    8610-83575262    jiyou_2000@163.com    Principal Investigator: Yong Huo, MD Less <<
NCT00972907 Chronic Anal Fissure Phase 1 Phase 2 Completed - Israel ... More >> Sapir Medical Center Kfar Saba, Israel Macabi HMO Tel Aviv, Israel Less <<
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Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

2.89mL

0.58mL

0.29mL

14.44mL

2.89mL

1.44mL

28.87mL

5.77mL

2.89mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1

References

 

Historical Records

Categories