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Chemical Structure| 29049-22-7 Chemical Structure| 29049-22-7

Structure of 29049-22-7

Chemical Structure| 29049-22-7

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Product Details of [ 29049-22-7 ]

CAS No. :29049-22-7
Formula : C10H12N4O5
M.W : 268.23
SMILES Code : OC[C@@H]1[C@H](C[C@H](N2C=NC3=C2N=C(O)N=C3O)O1)O
MDL No. :MFCD18643182

Safety of [ 29049-22-7 ]

Application In Synthesis of [ 29049-22-7 ]

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

  • Downstream synthetic route of [ 29049-22-7 ]

[ 29049-22-7 ] Synthesis Path-Downstream   1~1

  • 1
  • [ 961-07-9 ]
  • [ 55514-92-6 ]
  • (R)-8-(2-pyrrolidyl)-2'-deoxyguanosine [ No CAS ]
  • (S)-8-(2-pyrrolidyl)-2'-deoxyguanosine [ No CAS ]
  • [ 29049-22-7 ]
  • [ 88847-89-6 ]
  • [ 88899-00-7 ]
YieldReaction ConditionsOperation in experiment
In aq. acetate buffer; at 4℃; for 12h;pH 3.7;Acidic conditions; UV-irradiation; dG (10mM) was irradiated in sodium acetate buffer (3M, pH 3.7) in the presence of NPRO (100mM) in a chamber kept at 4C. Continuous mixing was done during the reaction. UVA irradiation was performed for 12h with 20-W black-light bulbs emitting radiation in the range 300-400nm (Panasonic, Japan). Use of a 4-mm thick glass plate between the light source and the reaction solution excluded radiation of wavelength nm. The UVA dose rate at 360nm on the surface of the solution, measured using a black-ray UV intensity meter (Ultraviolet Products, San Gabriel, CA), was 0.98±0.060mW/cm2. Irradiated samples were fractionated using HPLC (TSK-GEL ODS-80Ts, 21.5mm×300mm) equipped with a photodiode array detector SPP-M 10Avp (Shimadzu, Kyoto). These fractions were analyzed by LC-MSMS (API3000 and API4000, AB SCIEX, MA, USA) with positive mode ESI. The column used was Inertsil ODS-3 (1.0mm×150mm), and the eluent was 5mM ammonium acetate with 0-40% methanol. Co-chromatography with authentic specimen was performed with 10muL of irradiated sample mixed with 1nmol of dX, 1nmol of dO or 2nmol of 8-oxodG. 1H NMR spectra of samples dissolved in dimethylsulfoxide-d6 were recorded on a Unity INOVA AS600 spectrometer (Agilent Technologies, Tokyo) at 600MHz.
 

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