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Accessible (Haz class 3, 4, 5 or 8), Domestic USD 100+
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Chemical Structure| 370-86-5 Chemical Structure| 370-86-5

Structure of FCCP
CAS No.: 370-86-5

Chemical Structure| 370-86-5

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FCCP is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.

Synonyms: Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone; Trifluoromethoxy carbonylcyanide phenylhydrazone; para-Trifluoromethoxyphenylhydrazone, Carbonyl Cyanide.

4.5 *For Research Use Only !

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Nova-Baza, Daniela ; Olivares-Caro, Lia ; Vallejos, Alejandro ; Mennickent, Daniela ; Sáez-Orellana, Francisco ; Bustamante, Luis , et al.

Abstract: Berberis microphylla G Forst (Calafate) have been used in traditional medicine from prehispanic times in Patagonia. In the last decade the consumption of the fruit has been increased due to their antioxidant capacity, and because several studies demonstrated health benefits associated with the protection against atherosclerosis and other metabolic diseases. Nevertheless, the bioactivity properties of the leaves, a by-product of agronomic management, have been poorly studied. Recently, 108 compounds mainly hydroxycinnamic acids, flavonols, and were identified in a methanolic extract of the leaves, demonstrating great potential for the development of new functional beverages. Based on these, for first time a comprehensive chemical characterization and bioactivity was evaluated for a Calafate leaves infusion prepared in hot water. For this, chemical characterization of the infusion was performed by UHPLC-Q-TOF and TXRF. Bioactivity was assayed by antioxidant capacity, cell cytotoxicity, and cell oxidative stress assays. Inhibition of both Aβ aggregation for Alzheimer's disease and gastrointestinal enzymes for metabolic syndromes were evaluated. The results show that the infusion is rich in hydroxycinnamic acids and other bioactive compounds. The infusion does not contain toxic metals or cytotoxicity activity. The infusion can reduce intracellular reactive oxygen species in HUVEC cells and showed a reduction in the Aβ aggregation being a potential beverage for Alzheimer's prevention. Finally, the infusion had in-vitro hypoglycemic and hypolipidemic effects. These results support the usage of Berberis microphylla G Forst leaves as a new functional beverage.

Keywords: Bioactivity ; Calafate leaves ; Infusion ; metabolic syndrome ; Alzheimer's disease

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Salu, Philip ; Tuvin, Daniel ; Reindl, Katie M ;

Abstract: Anterior gradient 2 (AGR2) is often overexpressed in many human cancers, including pancreatic ductal adenocarcinoma (PDAC). Elevated AGR2 expression is known to play a critical role in tumor development, progression, and metastasis and positively correlates with poor patient survival. However, the relationship between AGR2 expression and tumor growth is not fully understood. Our study aims to investigate the impact of AGR2 knockdown on the survival of two pancreatic cancer cell lines, HPAF–II and PANC–1, that exhibit high AGR2 expression. This study revealed that the knockdown of AGR2 expression through an inducible shRNA-mediated approach reduced the proliferative ability and colony-forming potential of PDAC cells compared to scramble controls. Significantly, knocking down AGR2 led to the inhibition of multiple protein biosynthesis pathways and induced ER stress through unfolded protein response (UPR)activation. AGR2 knockdown induced ER stress and increased mitochondrial fission, while mitochondrial fusion remained unaffected. Ultimately, apoptotic cell death was heightened in AGR2 knockdown PDAC cells compared to the controls. Overall, these data reveal a new axis involving AGR2-ER stress-associated mitochondrial fission that could be targeted to improve PDAC patient outcomes.

Keywords: Pancreatic ductal adenocarcinoma (PDAC) ; anterior gradient 2 (AGR2) ; ER stress ; unfolded protein response (UPR) ; mitochondria dysfunction

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Alternative Products

Product Details of FCCP

CAS No. :370-86-5
Formula : C10H5F3N4O
M.W : 254.17
SMILES Code : N#C/C(C#N)=N/NC1=CC=C(OC(F)(F)F)C=C1
Synonyms :
Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone; Trifluoromethoxy carbonylcyanide phenylhydrazone; para-Trifluoromethoxyphenylhydrazone, Carbonyl Cyanide.
MDL No. :MFCD00009699
InChI Key :BMZRVOVNUMQTIN-UHFFFAOYSA-N
Pubchem ID :3330

Safety of FCCP

GHS Pictogram:
Signal Word:Danger
Hazard Statements:H301+H311+H331-H315-H319-H413
Precautionary Statements:P501-P261-P273-P270-P271-P264-P280-P337+P313-P305+P351+P338-P361+P364-P332+P313-P301+P310+P330-P302+P352+P312-P304+P340+P311-P403+P233-P405
Class:6.1
UN#:3439
Packing Group:

Isoform Comparison

Biological Activity

In Vitro:

Cell Line
Concentration Treated Time Description References
Nix−/− reticulocytes 10 µM 24 hours Promoted the clearance of mitochondria and restored the entry of mitochondria into autophagic vacuoles. PMC2570948
wild type Drosophila ovaries 10 μM completely abolished mtDNA replication throughout the germarium PMC3976679
wild type Drosophila ovaries 5 μM had no obvious effect on mtDNA replication in region 1, but suppressed replication in regions 2 and 3, with strong suppression in region 2B PMC3976679
wild type Drosophila ovaries 2 μM had no obvious effect on mtDNA replication in region 1, but suppressed replication in regions 2 and 3, with strong suppression in region 2B PMC3976679
PINK1−/− mouse embryonic fibroblasts (MEFs) 1 μM To study the amount of calcium released into the cytosol following FCCP treatment in PINK1−/− cells, the results showed significantly higher calcium release in PINK1−/− cells compared to wild-type cells. PMC3405481
PINK1−/− cortical neurons 1 μM To study the amount of calcium released into the cytosol following FCCP treatment in PINK1−/− neurons, the results showed significantly higher calcium release in PINK1−/− neurons compared to wild-type neurons. PMC3405481
LT-HSC 5 µM 5 days FCCP treatment significantly enhanced long-term multi-lineage blood reconstitution levels, indicating that FCCP reversibly modulates HSC metabolism through induction of autophagy, maintaining their self-renewal capacity. PMC5064016
primary brown adipocytes 1 μM Used to measure mitochondrial respiration rate, FCCP was used to uncouple the mitochondrial membrane and stimulate respiration PMC6287687
fibroblasts 0.5 μM to assess maximal mitochondrial respiration capacity PMC9542960
WT CPCs 25 μM 24 h To assess FCCP-induced mitophagy, results showed that WT CPCs had a robust increase in autophagy and mitochondrial clearance after FCCP treatment. PMC6613840
POLG CPCs 25 μM 24 h To assess FCCP-induced mitophagy, results showed that POLG CPCs had a robust increase in autophagy and mitochondrial clearance after FCCP treatment. PMC6613840
C2C12 myotubes 1 µM 6 hours Used to measure mitochondrial respiration, FCCP was used to uncouple mitochondrial respiration to determine the maximal respiration rate. PMC9225428

In Vivo:

Species
Animal Model
Administration Dosage Frequency Description References
Mice High-fat diet-induced obese mice In vitro experiment 40 mg/kg twice weekly for 3 weeks Used to measure mitochondrial respiration, FCCP was used to uncouple mitochondrial respiration to determine the maximal respiration rate. PMC9225428

Clinical Trial:

NCT Number Conditions Phases Recruitment Completion Date Locations
NCT00613860 Emphysema COP... More >>D Less << Phase 3 Unknown December 2011 Germany ... More >> Charite, University of Berlin Recruiting Berlin, Germany, 10117 Contact: Christian Witt, MD, PhD    +49 (030) 450 565 022    pneumologie@charite.de    Contact: Bernd Schmitt, MD    +49 (030) 450 565 02    pneumologie@charite.de    Thoraxklinik, University of Heidelberg Recruiting Heidelberg, Germany, 69190 Principal Investigator: Felix JF Herth, MD, PhD          Medical Center Nürnberg Recruiting Nürnberg, Germany, 90340 Contact: Joachim Ficker, MD, PHD    +49 (911) 398-2675    ficker@klinikum-nuernberg.de    Contact: Manfred Wagner, MD    +49 (911) 398-0    Manfred.Wagner@klinikum-nuernberg.de Less <<
NCT00176540 Fatigue Unspe... More >>cified Adult Solid Tumor, Protocol Specific Unspecified Childhood Solid Tumor, Protocol Specific Less << Not Applicable Terminated(slow accrual) - United States, New Jersey ... More >> Cancer Institute of New Jersey at Hamilton Hamilton, New Jersey, United States, 08690 Cancer Institute of New Jersey at UMDNJ - Robert Wood Johnson Medical School New Brunswick, New Jersey, United States, 08903 Less <<
NCT01187147 Asthma Not Applicable Completed - Hong Kong ... More >> University of Hong Kong Queen Mary Hospital Hong Kong, Hong Kong, 0 Less <<
NCT00842023 - Completed - -
NCT00430664 Malignant Pleural Effusions ... More >> Recurrent Pleural Effusions Primary Spontaneous Pneumothorax Secondary Spontaneous Pneumothorax Less << Not Applicable Unknown - India ... More >> PGIMER Recruiting Chandigarh, India, 160 012 Contact: Abinash Paul, MD    91-9417745955    draspaul@yahoo.co.in    Contact: Ritesh Agarwal, MD DM    91- 9815799226    ritesh@indiachest.org Less <<
NCT00842023 Acute Decompensated Heart Fail... More >>ure Less << Phase 4 Completed - United States, California ... More >> Centinela Hospital Medical Center Inglewood, California, United States, 90301 Less <<
NCT00895115 Prostate Cancer Phase 1 Completed - United States, New Jersey ... More >> Cancer Institute of New Jersey at UMDNJ - Robert Wood Johnson Medical School New Brunswick, New Jersey, United States, 08903 Less <<
NCT00905918 Colorectal Cancer Phase 1 Completed - United States, New Jersey ... More >> Rutgers Cancer Institute of New Jersey New Brunswick, New Jersey, United States, 08903 Less <<
NCT01149863 Autologous Transplantation Phase 2 Completed - United States, Georgia ... More >> Emory University Winship Cancer Institute Atlanta, Georgia, United States, 30322 Less <<
NCT01121055 Patient Satisfaction for Bronc... More >>hoscopy Less << Phase 4 Unknown December 2010 Korea, Republic of ... More >> Seoul National University Hospital Recruiting Seoul, Korea, Republic of, 110-744 Contact: Seok-Chul Yang, MD       scyang@snu.ac.kr    Contact: Jong Sun Park, MD       jspark.im@gmail.com Less <<
NCT00963716 Endobronchial Growth ... More >> Lung Cancer Less << Not Applicable Completed - India ... More >> Department of Pulmonary Medicine, PGIMER, India Chandigarh, UT, India, 160012 Less <<
NCT00226746 Carcinoma, Pancreatic Ductal Phase 2 Completed - United States, New York ... More >> New York Methodist Hospital Brooklyn, New York, United States, 11215 Less <<
NCT01149863 - Completed - -
NCT00736801 Allergic Asthma Not Applicable Completed - Germany ... More >> University of Rostock Rostock, Germany, 18057 Less <<
NCT00550602 - Completed - United States, Massachusetts ... More >> Beth Israel Deaconess Medical Center- Division of Interventional Pulmonology Boston, Massachusetts, United States, 02215 Less <<
NCT01459926 Opioid Induced Constipation Phase 2 Completed - United States, Alabama ... More >> G and L Research, LLC Foley, Alabama, United States, 36535 Less <<
NCT01441570 - Terminated(due to slow enrollm... More >>ent) Less << - -
NCT01401985 Opioid Induced Constipation Phase 2 Completed - United States, California ... More >> Pasadena, California, United States Less <<
NCT00374751 Cancer Metast... More >>asis Pain Less << Phase 1 Phase 2 Completed - United States, New York ... More >> New York Methodist Hospital Brooklyn, New York, United States, 11215 Less <<
NCT00881335 Pulmonary Disease Not Applicable Completed - -
NCT01441570 Transplant; Failure, Kidney ... More >> Hypertension Less << Not Applicable Terminated(due to slow enrollm... More >>ent) Less << - United States, Massachusetts ... More >> Brigham and Women's Hospital Boston, Massachusetts, United States, 02115 Lahey Clinic Burlington, Massachusetts, United States, 01805 Less <<
NCT00881335 - Completed - -
NCT02146092 Lung Neoplasms ... More >> Benign Lung Disease Less << Phase 2 Completed - Canada, Ontario ... More >> St. Joseph's Healthcare Hamilton Hamilton, Ontario, Canada, L8N 4A6 Less <<
NCT02149576 Lung Neoplasms Phase 2 Active, not recruiting April 30, 2018 Canada, Ontario ... More >> St. Joseph's Healthcare Hamilton Hamilton, Ontario, Canada, L8N 4A6 Less <<
NCT01220973 - Completed - -
NCT01220973 Prostate Cancer Phase 2 Completed - United States, Michigan ... More >> Karmanos Cancer Center Detroit, Michigan, United States, 48201 United States, New Jersey Cooper Hospital Camden, New Jersey, United States, 08103 Robert Wood Johnson University Hospital at Hamilton Hamilton, New Jersey, United States, 08690 Rutgers Cancer Institute of New Jersey New Brunswick, New Jersey, United States, 08903 Less <<
NCT01130311 Tuberculosis, Pulmonary Not Applicable Completed - Pakistan ... More >> Aga Khan University Karachi, Sind, Pakistan, 74800 Ojha Istitute of Chest Diseases Karachi, Sind, Pakistan, 74800 Abbasi Shaheed Hospital Karachi, Sind, Pakistan, 74812 Malir Chest Clinic Karachi, Sind, Pakistan, 74831 Less <<
NCT02025205 COPD Patients With Homogeneous... More >> Emphysema Endoscopic Lung Volume Reduction With Endobronchial Valve Less << Not Applicable Completed - Austria ... More >> Ludwig-Boltzmann-Institut für COPD und Pneumologische Epidemiologie Vienna, Austria, 1140 Germany Charité Campus Virchow-Klinikum Berlin, Germany, 13353 Ruhrlandklinik, Westdeutsches Lungenzentrum Essen, Germany, 45239 Lungenabteilung Thoraxzentrum Hamburg Hamburg, Germany, 21075 Thoraxklinik am Universitäts klinikum Heidelberg Heidelberg, Germany, 69126 Lungenklinik Hemer Hemer, Germany, 58675 Klinikum Nürnberg Nord Nürnberg, Germany, 90419 Netherlands Department of pulmonary dieases, University Medical Center Groningen, Netherlands, 9700RB Less <<
NCT01494896 - Terminated(due to slow enrollm... More >>ent. United Therapeutics and Dr. Bajwa MD decided to terminate study) Less << - United States, Florida ... More >> University of Florida, Jacksonville Jacksonville, Florida, United States, 32209 Mayo Clinic Jacksonville, Florida, United States, 32224 Cleveland Clinic Florida Weston, Florida, United States, 33331 Less <<
NCT02517359 Asthma Health... More >>y Volunteers Less << Phase 1 Terminated(Preset pharmacokine... More >>tic stopping criteria met at lower doses than expected.) Less << - United Kingdom ... More >> Manchester, United Kingdom, M23 9QZ Nottingham, United Kingdom, NG11 6JS Less <<
NCT01993914 - Completed - United States, California ... More >> The Elite Laser Vein Center Los Angeles, California, United States, 90025 Less <<
NCT02334007 Venous Thromboembolism ... More >> Lung Neoplasms Pulmonary Embolism Less << Phase 1 Phase 2 Completed - Canada, Ontario ... More >> St. Joseph's Healthcare Hamilton Hamilton, Ontario, Canada, L8N 4A6 Toronto General Hospital Toronto, Ontario, Canada, M5G 2C4 Less <<
NCT02663596 Pediatric Continuous Renal Rep... More >>lacement Therapy Dialysis Renal Failure Less << Phase 1 Recruiting December 2020 United States, Pennsylvania ... More >> St. Christopher's Hospital for Children Recruiting Philadelphia, Pennsylvania, United States, 19134 Contact: Jeffrey J Cies, PharmD, MPH, BCPS-AQ ID, BCPPS    215-427-5176    jeffrey.cies@tenethealth.com Less <<
NCT01805726 Bronchoscopy ... More >>Conscious Sedation Less << Phase 3 Completed - Lebanon ... More >> Hotel Dieu de France Hospital Beirut, Lebanon Less <<
NCT03672149 Renal Replacement Therapy ... More >> Pharmacokinetics Less << Phase 1 Not yet recruiting December 31, 2021 -
NCT00919152 - Completed - United States, Nebraska ... More >> The Nebraska Medical Center Omaha, Nebraska, United States, 68198 Less <<
NCT01889758 Kidney Transplant Recipients ... More >> Liver Transplant Recipients Less << Phase 4 Completed - United States, Ohio ... More >> The Christ Hospital Cincinnati, Ohio, United States, 45267 University of Cincinnati Cincinnati, Ohio, United States, 45267 Less <<
NCT01953081 Enteral Feeding Intolerance Phase 1 Phase 2 Completed - Australia, South Australia ... More >> Royal Adelaide Hospital Adelaide, South Australia, Australia Less <<
NCT01953081 - Completed - -
NCT03466385 Chronic Obstructive Pulmonary ... More >>Disease Respiratory Failure Acute Exacerbation Copd Less << Not Applicable Recruiting December 31, 2020 Greece ... More >> Evangelismos Hospital Recruiting Athens, Greece, 10673 Contact: Eleni Ischaki    +306974856544    eischaki@yahoo.gr Less <<
NCT02685930 - Completed - United States, Missouri ... More >> Barnes-Jewish Hospital Saint Louis, Missouri, United States, 63110 Less <<
NCT02097212 - Completed - United States, Colorado ... More >> National Jewish Health Denver, Colorado, United States, 80206 Less <<
NCT00862862 - Terminated(lack of enrollment) - United States, Nebraska ... More >> The Nebraska Medical Center Omaha, Nebraska, United States, 68198 Less <<
NCT02105012 Asthma Phase 2 Completed - -
NCT00881218 Ischemic Heart Disease Early Phase 1 Completed - United States, Missouri ... More >> Washington University School of Medicine St. Louis, Missouri, United States, 63110 Less <<
NCT03531944 Diabetes Mellitus, Type 2 Not Applicable Recruiting May 31, 2020 Singapore ... More >> Keat Hong Family Medicine Clinic Recruiting Singapore, Singapore, 689687 Contact: Joyce Lee Less <<
NCT02505685 - Active, not recruiting July 2020 Israel ... More >> Rabin Medical Center Petach Tikva, Israel, 49100 Less <<
NCT02755337 - Unknown December 2016 Indonesia ... More >> Dr. Soetomo General Hospital Recruiting Surabaya, East Java, Indonesia, 60286 Contact: Laksmi Wulandari, MD. PhD    +628123019591    laksmigts@yahoo.co.id    Principal Investigator: Laksmi Wulandari, MD, PhD Less <<
NCT03047941 - Suspended(Lack of participants... More >>) Less << October 31, 2022 Belgium ... More >> ASTES Bouge, Namur, Belgium, 5001 Less <<
NCT02105012 - Completed - -
NCT03752437 Extracorporeal Circulation; Co... More >>mplications Less << Phase 4 Not yet recruiting March 1, 2020 -
NCT03574740 - Completed - -
NCT02682030 Amyotrophic Lateral Sclerosis Not Applicable Recruiting December 2018 United States, California ... More >> Cedars-Sinai Medical Center Recruiting Los Angeles, California, United States, 90048 Contact: Dana Fine    310-423-1791 Less <<
NCT03102684 Second Hand Tobacco Smoke Not Applicable Active, not recruiting October 2019 Greece ... More >> "George D Behrakis RESEARCH LAB" of the Hellenic Cancer Society (HCS) Athens, Greece, 1152 Less <<
NCT03743090 - Not yet recruiting April 10, 2020 -
NCT00758316 Malignant Pleural Effusions Phase 3 Completed - Singapore ... More >> Sinagpore General Hospital Singapore, Singapore, 169608 Less <<
NCT03696524 Pleural Effusions, Chronic ... More >> Congestive Heart Failure Shortness of Breath Less << Not Applicable Not yet recruiting September 30, 2020 -
NCT01386203 - Unknown May 2018 Israel ... More >> Sheba Medical Center Recruiting Tel Hashomer, Israel Contact: Nir Peled       peled.nir@sheba.health.gov.il Less <<
NCT00719784 Respiratory Diseases ... More >> Pulmonary Diseases Thoracic Diseases Lung Diseases Less << Not Applicable Completed - Singapore ... More >> Singapore General Hospital Singapore, Singapore Less <<
NCT03694847 - Completed - Canada, Quebec ... More >> Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec Québec, Quebec, Canada, G1V 4G5 Less <<
NCT00526019 - Completed - -
NCT00532831 - Completed - Canada, Quebec ... More >> Centre de Recherche, Hôpital Laval Québec, Quebec, Canada, G1V 4G5 Less <<
NCT00532363 - Completed - Canada, Quebec ... More >> Centre de Recherche, Hôpital Laval Québec, Quebec, Canada, G1V 4G5 Less <<
NCT02338895 - Unknown September 2015 Saudi Arabia ... More >> King Faisal specialist Hospital & Research Center Recruiting Riyadh, Ar Riyadh, Saudi Arabia, 11211 Contact: Iqbal Hussain, MD    966533215378    hiqbal@kfshrc.edu.sa    Contact: Nawal Salahuddin, MD,FCCP    966551948786    nsalahuddin@kfshrc.edu.sa Less <<
NCT03118765 Mild to Moderate Chronic Obstr... More >>uctive Pulmonary Disease (COPD) Less << Phase 2 Completed - United States, Alabama ... More >> Glenmark Investigational Site 23 Andalusia, Alabama, United States, 36420 United States, Arizona Glenmark Investigational Site 12 Phoenix, Arizona, United States, 85006 Glenmark Investigational Site 14 Tempe, Arizona, United States, 85283 United States, California Glenmark Investigational Site 17 Fullerton, California, United States, 92835 United States, Florida Glenmark Investigational Site 19 Edgewater, Florida, United States, 32132 Glenmark Investigational Site 10 Miami Lakes, Florida, United States, 33016 Glenmark Investigational Site 24 Miami, Florida, United States, 33144 Glenmark Investigational Site 16 Miami, Florida, United States, 33186 Glenmark Investigational Site 6 Orlando, Florida, United States, 32825 Glenmark Investigational Site 20 Ormond Beach, Florida, United States, 32124 Glenmark Investigational Site 21 Vero Beach, Florida, United States, 32960 Glenmark Investigational Site 13 Winter Park, Florida, United States, 32789 United States, Nevada Glenmark Investigational Site 18 Las Vegas, Nevada, United States, 89119 United States, North Carolina Glenmark Investigational Site 9 Charlotte, North Carolina, United States, 28207 United States, Ohio Glenmark Investigational Site 5 Columbus, Ohio, United States, 43213 Glenmark Investigational Site 22 Columbus, Ohio, United States, 43215 Glenmark Investigational Site 8 Dublin, Ohio, United States, 43016 United States, Oregon Glenmark Investigational Site 11 Medford, Oregon, United States, 97504-9741 United States, South Carolina Glenmark Investigational Site 3 Easley, South Carolina, United States, 29640 Glenmark Investigational Site 2 Greenville, South Carolina, United States, 29615 Glenmark Investigational Site 4 Greenville, South Carolina, United States, 29615 Glenmark Investigational Site 1 Rock Hill, South Carolina, United States, 29732 Glenmark Investigational Site 15 Spartanburg, South Carolina, United States, 29303 Glenmark Investigational Site 7 Spartanburg, South Carolina, United States, 29303 United States, Texas Glenmark Investigational Site 25 Tomball, Texas, United States, 77375 Less <<
NCT03327701 Asthma, Exercise-Induced Phase 3 Not yet recruiting April 2020 -
NCT00374803 - Completed - -
NCT02851927 Pleural Effusion Not Applicable Recruiting September 2018 India ... More >> All India Institute of Medical Sciences Recruiting New Delhi, India, 110029 Contact: Karan Madan, MD, DM    +91-11-26593676    drkaranmadan@gmail.com Less <<
NCT00374803 End Stage Renal Disease (ESRD) Phase 4 Completed - United States, Ohio ... More >> The Christ Hospital Cincinnati, Ohio, United States, 45267 University of Cincinnati Cincinnati, Ohio, United States, 45267 Less <<
NCT00441389 - Completed - -
NCT02820051 Conscious Sedation Failure Dur... More >>ing Procedure Less << Not Applicable Completed - Mexico ... More >> UANL University Hospital Monterrey, Nuevo León, Mexico, 64000 Less <<
NCT02516449 Asthma Not Applicable Completed - Canada, Quebec ... More >> Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec Québec, Quebec, Canada, G1V 4G5 Less <<
NCT00532896 - Completed - Canada, Quebec ... More >> Centre de Recherche, Hôpital Laval Québec, Quebec, Canada, G1V 4G5 Less <<
NCT02301923 - Unknown March 2015 Lebanon ... More >> Hotel Dieu de France Hospital Not yet recruiting Beirut, Lebanon Contact: Moussa A Riachy, MD, FCCP    961 1 615300 ext 9218    riachy@dm.net.lb    Contact: Mirella Skandar, MD    96170231331    mirella.iskandar@gmail.com    Sub-Investigator: Jad Choucair, MD          Sub-Investigator: Samer Najem, MD Less <<
NCT01067755 - Unknown March 2011 Germany ... More >> Thoraxklinik Recruiting Heidelberg, Germany Contact: Felix Herth       Felix.Herth@thoraxklinik-heidelberg.de    Principal Investigator: Felix JF Herth, MD, PhD, FCCP Less <<
NCT02389361 Pain Phase 4 Completed - -
NCT00878371 Dissecting Aneurysm of the Tho... More >>racic Aorta Less << Phase 4 Completed - Canada, Nova Scotia ... More >> Capital Health- Queen Elizabeth II Health Sciences Centre Halifax, Nova Scotia, Canada, B3H3A7 Less <<
NCT02389361 - Completed - -
NCT02338284 - Unknown September 2015 Saudi Arabia ... More >> King Faisal Specialist Hospital & Research Centre Recruiting Riyadh, Ar-Riyadh, Saudi Arabia, 11211 Contact: Alaa Mohamed, MD    966553730044    dr.alaibrahim@gmail.com    Contact: nawal salahuddin, MD,FCCP    966551948786    nsalahuddin@kfshrc.edu.sa    Sub-Investigator: Nadia AlHarbi, MD Less <<
NCT00833300 Delirium Agit... More >>ation Less << Not Applicable Terminated - Canada, Nova Scotia ... More >> Halifax Infirmary; Queen Elizabeth II Health Sciences Centre Halifax, Nova Scotia, Canada Victoria General Hospital; Queen Elizabeth II Health Sciences Centre Halifax, Nova Scotia, Canada Less <<
NCT02824952 Lung Cancer Phase 2 Not yet recruiting July 2020 Israel ... More >> Rabin Medical Center Petach Tikva, Israel, 49100 Less <<
NCT03570580 - Recruiting December 31, 2018 Belgium ... More >> Clinique Saint-Luc Bouge Recruiting Namur, Belgium, 5004 Contact: Eric P Deflandre, MD, FCCP    0032477764241    eric.deflandre@gmail.com Less <<
NCT03047421 - Completed - Belgium ... More >> Clinique Saint-Luc of Bouge Bouge, Namur, Belgium, 5004 Less <<
NCT03047850 - Completed - Belgium ... More >> Clinique Saint-Luc of Bouge Bouge, Namur, Belgium, 5001 Less <<
NCT03744455 - Recruiting February 1, 2019 Belgium ... More >> Clinique Saint-Luc Bouge Recruiting Namur, Belgium, 5004 Contact: Eric P Deflandre, MD, FCCP    0032477764241    eric.deflandre@gmail.com Less <<
NCT02045121 Pleural Effusion, Malignant Not Applicable Unknown May 2015 Singapore ... More >> Division of Respiratory and Critical Care Medicine, National University Hospital Recruiting Singapore, Singapore, 119228 Contact: Pyng Lee    65-67726533    mdclp@nus.edu.sg    Principal Investigator: Pyng Lee, MBBS, MRCP, MMED, FAMS, FCCP Less <<
NCT01852838 - Unknown April 2015 Israel ... More >> Sheba Medical Center Recruiting Ramat Gan, Israel, 52621 Contact: Eve Keret    972-3-530-3207    eve.keret@sheba.health.gov.il    Principal Investigator: Dr. Nir Peled, PhD,MD, FCCP Less <<
NCT01527461 - Unknown - Israel ... More >> Sheba Medical Center Recruiting Tel Hasomer, Israel, 52621 Contact: Nir Peled, MD Phd FCCP    97235307014    nir.peled@gmail.com    Contact: Michal Alumot, M.Sc,RD    97235307009    michalalumot@gmail.com    Sub-Investigator: Tal Frenkel, B.Sc Less <<
NCT01360931 - Unknown June 2013 Hong Kong ... More >> Queen Mary Hospital Recruiting Hong Kong, Hong Kong, 0 Contact: David CL Lam, MBBS, FRCP(Edin), FCCP, FACP    +852 2255 5814    dcllam@hku.hk Less <<
NCT01460108 Pulmonary Emphysema ... More >> COPD Lung Diseases Less << Phase 4 Unknown June 2013 Austria ... More >> Otto-Wagner-Spital Recruiting Wien, Austria, 1140 Contact: Arschang Valipour, MD, FCCP    43-1-91060-41008    arschang.valipour@wienkav.at    Principal Investigator: Arschang Valipour, MD, FCCP Less <<
NCT01185652 - Unknown September 2012 Hong Kong ... More >> University of Hong Kong Queen Mary Hospital Recruiting Hong Kong, Hong Kong, 0 Contact: David CL Lam, BSc,MBBS,PhD,FCCP,FACP,FRCP(E)    (852) 2255 5814    dcllam@hku.hk    Principal Investigator: David CL Lam, BSc,MBBS,PhD,FCCP,FACP,FRCP(E) Less <<
NCT02049255 Pain Phase 4 Completed - Belgium ... More >> Clinique Saint-Luc Bouge Bouge, Namur, Belgium, 5004 Less <<
NCT02051829 - Completed - Belgium ... More >> Cabinet Medical ASTES Jambes, Belgium, 5100 Less <<
NCT01351155 Acute Respiratory Failure Not Applicable Unknown October 2015 Italy ... More >> Pulmonary Division With Respiratory Intensive Care Unit, S. Donato Hospital, Arezzo, Italy Recruiting Lucca, Italy, 55100 Contact       raffaele_scala@hotmail.com    Principal Investigator: Raffaele Scala, MD, FCCP Less <<
NCT02202122 - Withdrawn(lack of investigator... More >>s) Less << October 2015 Belgium ... More >> Clinique Saint-Luc de Bouge Namur, Belgium, 5100 Less <<
NCT02507778 Lung Cancer Not Applicable Recruiting September 2019 Israel ... More >> Rabin Medical Center Recruiting Petach Tikva, Israel, 49100 Contact: Nir Peled, MD PhD FCCP       nirp@clalit.org.il Less <<
NCT02198820 - Withdrawn(lack of investigator... More >>s) Less << September 2015 Belgium ... More >> Clinique Saint-Luc de Bouge Namur, Belgium, 5004 Less <<
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NCT02282280 - Completed - Belgium ... More >> Clinique Saint-Luc Bouge Namur, Belgium, 5004 Less <<
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Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

3.93mL

0.79mL

0.39mL

19.67mL

3.93mL

1.97mL

39.34mL

7.87mL

3.93mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2

References

 

Historical Records

Categories