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Structure of FCCP
CAS No.: 370-86-5
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The BI-3802 was designed by Boehringer Ingelheim and could be obtained free of charge through the Boehringer Ingelheim open innovation portal opnMe.com, associated with its negative control.
FCCP is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.
Synonyms: Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone; Trifluoromethoxy carbonylcyanide phenylhydrazone; para-Trifluoromethoxyphenylhydrazone, Carbonyl Cyanide.
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BIOACTIVITY EVALUATION FROM Berberis microphylla G. FORST (CALAFATE) LEAVES INFUSION
Nova-Baza, Daniela ; Olivares-Caro, Lia ; Vallejos, Alejandro ; Mennickent, Daniela ; Sáez-Orellana, Francisco ; Bustamante, Luis , et al.
Abstract: Berberis microphylla G Forst (Calafate) have been used in traditional medicine from prehispanic times in Patagonia. In the last decade the consumption of the fruit has been increased due to their antioxidant capacity, and because several studies demonstrated health benefits associated with the protection against atherosclerosis and other metabolic diseases. Nevertheless, the bioactivity properties of the leaves, a by-product of agronomic management, have been poorly studied. Recently, 108 compounds mainly hydroxycinnamic acids, flavonols, and berberine were identified in a methanolic extract of the leaves, demonstrating great potential for the development of new functional beverages. Based on these, for first time a comprehensive chemical characterization and bioactivity was evaluated for a Calafate leaves infusion prepared in hot water. For this, chemical characterization of the infusion was performed by UHPLC-Q-TOF and TXRF. Bioactivity was assayed by antioxidant capacity, cell cytotoxicity, and cell oxidative stress assays. Inhibition of both Aβ aggregation for Alzheimer's disease and gastrointestinal enzymes for metabolic syndromes were evaluated. The results show that the infusion is rich in hydroxycinnamic acids and other bioactive compounds. The infusion does not contain toxic metals or cytotoxicity activity. The infusion can reduce intracellular reactive oxygen species in HUVEC cells and showed a reduction in the Aβ aggregation being a potential beverage for Alzheimer's prevention. Finally, the infusion had in-vitro hypoglycemic and hypolipidemic effects. These results support the usage of Berberis microphylla G Forst leaves as a new functional beverage.
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Keywords: Bioactivity ; Calafate leaves ; Infusion ; metabolic syndrome ; Alzheimer's disease
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AGR2 knockdown induces ER stress and mitochondria fission to facilitate pancreatic cancer cell death
Salu, Philip ; Tuvin, Daniel ; Reindl, Katie M ;
Abstract: Anterior gradient 2 (AGR2) is often overexpressed in many human cancers, including pancreatic ductal adenocarcinoma (PDAC). Elevated AGR2 expression is known to play a critical role in tumor development, progression, and metastasis and positively correlates with poor patient survival. However, the relationship between AGR2 expression and tumor growth is not fully understood. Our study aims to investigate the impact of AGR2 knockdown on the survival of two pancreatic cancer cell lines, HPAF–II and PANC–1, that exhibit high AGR2 expression. This study revealed that the knockdown of AGR2 expression through an inducible shRNA-mediated approach reduced the proliferative ability and colony-forming potential of PDAC cells compared to scramble controls. Significantly, knocking down AGR2 led to the inhibition of multiple protein biosynthesis pathways and induced ER stress through unfolded protein response (UPR)activation. AGR2 knockdown induced ER stress and increased mitochondrial fission, while mitochondrial fusion remained unaffected. Ultimately, apoptotic cell death was heightened in AGR2 knockdown PDAC cells compared to the controls. Overall, these data reveal a new axis involving AGR2-ER stress-associated mitochondrial fission that could be targeted to improve PDAC patient outcomes.
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Keywords: Pancreatic ductal adenocarcinoma (PDAC) ; anterior gradient 2 (AGR2) ; ER stress ; unfolded protein response (UPR) ; mitochondria dysfunction
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CAS No. : | 370-86-5 |
Formula : | C10H5F3N4O |
M.W : | 254.17 |
SMILES Code : | N#C/C(C#N)=N/NC1=CC=C(OC(F)(F)F)C=C1 |
Synonyms : |
Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone; Trifluoromethoxy carbonylcyanide phenylhydrazone; para-Trifluoromethoxyphenylhydrazone, Carbonyl Cyanide.
|
MDL No. : | MFCD00009699 |
InChI Key : | BMZRVOVNUMQTIN-UHFFFAOYSA-N |
Pubchem ID : | 3330 |
GHS Pictogram: |
![]() |
Signal Word: | Danger |
Hazard Statements: | H301+H311+H331-H315-H319-H413 |
Precautionary Statements: | P501-P261-P273-P270-P271-P264-P280-P337+P313-P305+P351+P338-P361+P364-P332+P313-P301+P310+P330-P302+P352+P312-P304+P340+P311-P403+P233-P405 |
Class: | 6.1 |
UN#: | 3439 |
Packing Group: | Ⅲ |
In Vitro:
Concentration | Treated Time | Description | References |
Nix−/− reticulocytes | 10 µM | 24 hours | Promoted the clearance of mitochondria and restored the entry of mitochondria into autophagic vacuoles. | PMC2570948 |
wild type Drosophila ovaries | 10 μM | completely abolished mtDNA replication throughout the germarium | PMC3976679 | |
wild type Drosophila ovaries | 5 μM | had no obvious effect on mtDNA replication in region 1, but suppressed replication in regions 2 and 3, with strong suppression in region 2B | PMC3976679 | |
wild type Drosophila ovaries | 2 μM | had no obvious effect on mtDNA replication in region 1, but suppressed replication in regions 2 and 3, with strong suppression in region 2B | PMC3976679 | |
PINK1−/− mouse embryonic fibroblasts (MEFs) | 1 μM | To study the amount of calcium released into the cytosol following FCCP treatment in PINK1−/− cells, the results showed significantly higher calcium release in PINK1−/− cells compared to wild-type cells. | PMC3405481 | |
PINK1−/− cortical neurons | 1 μM | To study the amount of calcium released into the cytosol following FCCP treatment in PINK1−/− neurons, the results showed significantly higher calcium release in PINK1−/− neurons compared to wild-type neurons. | PMC3405481 | |
LT-HSC | 5 µM | 5 days | FCCP treatment significantly enhanced long-term multi-lineage blood reconstitution levels, indicating that FCCP reversibly modulates HSC metabolism through induction of autophagy, maintaining their self-renewal capacity. | PMC5064016 |
primary brown adipocytes | 1 μM | Used to measure mitochondrial respiration rate, FCCP was used to uncouple the mitochondrial membrane and stimulate respiration | PMC6287687 | |
fibroblasts | 0.5 μM | to assess maximal mitochondrial respiration capacity | PMC9542960 | |
WT CPCs | 25 μM | 24 h | To assess FCCP-induced mitophagy, results showed that WT CPCs had a robust increase in autophagy and mitochondrial clearance after FCCP treatment. | PMC6613840 |
POLG CPCs | 25 μM | 24 h | To assess FCCP-induced mitophagy, results showed that POLG CPCs had a robust increase in autophagy and mitochondrial clearance after FCCP treatment. | PMC6613840 |
C2C12 myotubes | 1 µM | 6 hours | Used to measure mitochondrial respiration, FCCP was used to uncouple mitochondrial respiration to determine the maximal respiration rate. | PMC9225428 |
In Vivo:
Administration | Dosage | Frequency | Description | References |
Mice | High-fat diet-induced obese mice | In vitro experiment | 40 mg/kg | twice weekly for 3 weeks | Used to measure mitochondrial respiration, FCCP was used to uncouple mitochondrial respiration to determine the maximal respiration rate. | PMC9225428 |
Clinical Trial:
NCT Number | Conditions | Phases | Recruitment | Completion Date | Locations |
NCT00613860 | Emphysema COP... More >>D Less << | Phase 3 | Unknown | December 2011 | Germany ... More >> Charite, University of Berlin Recruiting Berlin, Germany, 10117 Contact: Christian Witt, MD, PhD +49 (030) 450 565 022 pneumologie@charite.de Contact: Bernd Schmitt, MD +49 (030) 450 565 02 pneumologie@charite.de Thoraxklinik, University of Heidelberg Recruiting Heidelberg, Germany, 69190 Principal Investigator: Felix JF Herth, MD, PhD Medical Center Nürnberg Recruiting Nürnberg, Germany, 90340 Contact: Joachim Ficker, MD, PHD +49 (911) 398-2675 ficker@klinikum-nuernberg.de Contact: Manfred Wagner, MD +49 (911) 398-0 Manfred.Wagner@klinikum-nuernberg.de Less << |
NCT00176540 | Fatigue Unspe... More >>cified Adult Solid Tumor, Protocol Specific Unspecified Childhood Solid Tumor, Protocol Specific Less << | Not Applicable | Terminated(slow accrual) | - | United States, New Jersey ... More >> Cancer Institute of New Jersey at Hamilton Hamilton, New Jersey, United States, 08690 Cancer Institute of New Jersey at UMDNJ - Robert Wood Johnson Medical School New Brunswick, New Jersey, United States, 08903 Less << |
NCT01187147 | Asthma | Not Applicable | Completed | - | Hong Kong ... More >> University of Hong Kong Queen Mary Hospital Hong Kong, Hong Kong, 0 Less << |
NCT00842023 | - | Completed | - | - | |
NCT00430664 | Malignant Pleural Effusions ... More >> Recurrent Pleural Effusions Primary Spontaneous Pneumothorax Secondary Spontaneous Pneumothorax Less << | Not Applicable | Unknown | - | India ... More >> PGIMER Recruiting Chandigarh, India, 160 012 Contact: Abinash Paul, MD 91-9417745955 draspaul@yahoo.co.in Contact: Ritesh Agarwal, MD DM 91- 9815799226 ritesh@indiachest.org Less << |
NCT00842023 | Acute Decompensated Heart Fail... More >>ure Less << | Phase 4 | Completed | - | United States, California ... More >> Centinela Hospital Medical Center Inglewood, California, United States, 90301 Less << |
NCT00895115 | Prostate Cancer | Phase 1 | Completed | - | United States, New Jersey ... More >> Cancer Institute of New Jersey at UMDNJ - Robert Wood Johnson Medical School New Brunswick, New Jersey, United States, 08903 Less << |
NCT00905918 | Colorectal Cancer | Phase 1 | Completed | - | United States, New Jersey ... More >> Rutgers Cancer Institute of New Jersey New Brunswick, New Jersey, United States, 08903 Less << |
NCT01149863 | Autologous Transplantation | Phase 2 | Completed | - | United States, Georgia ... More >> Emory University Winship Cancer Institute Atlanta, Georgia, United States, 30322 Less << |
NCT01121055 | Patient Satisfaction for Bronc... More >>hoscopy Less << | Phase 4 | Unknown | December 2010 | Korea, Republic of ... More >> Seoul National University Hospital Recruiting Seoul, Korea, Republic of, 110-744 Contact: Seok-Chul Yang, MD scyang@snu.ac.kr Contact: Jong Sun Park, MD jspark.im@gmail.com Less << |
NCT00963716 | Endobronchial Growth ... More >> Lung Cancer Less << | Not Applicable | Completed | - | India ... More >> Department of Pulmonary Medicine, PGIMER, India Chandigarh, UT, India, 160012 Less << |
NCT00226746 | Carcinoma, Pancreatic Ductal | Phase 2 | Completed | - | United States, New York ... More >> New York Methodist Hospital Brooklyn, New York, United States, 11215 Less << |
NCT01149863 | - | Completed | - | - | |
NCT00736801 | Allergic Asthma | Not Applicable | Completed | - | Germany ... More >> University of Rostock Rostock, Germany, 18057 Less << |
NCT00550602 | - | Completed | - | United States, Massachusetts ... More >> Beth Israel Deaconess Medical Center- Division of Interventional Pulmonology Boston, Massachusetts, United States, 02215 Less << | |
NCT01459926 | Opioid Induced Constipation | Phase 2 | Completed | - | United States, Alabama ... More >> G and L Research, LLC Foley, Alabama, United States, 36535 Less << |
NCT01441570 | - | Terminated(due to slow enrollm... More >>ent) Less << | - | - | |
NCT01401985 | Opioid Induced Constipation | Phase 2 | Completed | - | United States, California ... More >> Pasadena, California, United States Less << |
NCT00374751 | Cancer Metast... More >>asis Pain Less << | Phase 1 Phase 2 | Completed | - | United States, New York ... More >> New York Methodist Hospital Brooklyn, New York, United States, 11215 Less << |
NCT00881335 | Pulmonary Disease | Not Applicable | Completed | - | - |
NCT01441570 | Transplant; Failure, Kidney ... More >> Hypertension Less << | Not Applicable | Terminated(due to slow enrollm... More >>ent) Less << | - | United States, Massachusetts ... More >> Brigham and Women's Hospital Boston, Massachusetts, United States, 02115 Lahey Clinic Burlington, Massachusetts, United States, 01805 Less << |
NCT00881335 | - | Completed | - | - | |
NCT02146092 | Lung Neoplasms ... More >> Benign Lung Disease Less << | Phase 2 | Completed | - | Canada, Ontario ... More >> St. Joseph's Healthcare Hamilton Hamilton, Ontario, Canada, L8N 4A6 Less << |
NCT02149576 | Lung Neoplasms | Phase 2 | Active, not recruiting | April 30, 2018 | Canada, Ontario ... More >> St. Joseph's Healthcare Hamilton Hamilton, Ontario, Canada, L8N 4A6 Less << |
NCT01220973 | - | Completed | - | - | |
NCT01220973 | Prostate Cancer | Phase 2 | Completed | - | United States, Michigan ... More >> Karmanos Cancer Center Detroit, Michigan, United States, 48201 United States, New Jersey Cooper Hospital Camden, New Jersey, United States, 08103 Robert Wood Johnson University Hospital at Hamilton Hamilton, New Jersey, United States, 08690 Rutgers Cancer Institute of New Jersey New Brunswick, New Jersey, United States, 08903 Less << |
NCT01130311 | Tuberculosis, Pulmonary | Not Applicable | Completed | - | Pakistan ... More >> Aga Khan University Karachi, Sind, Pakistan, 74800 Ojha Istitute of Chest Diseases Karachi, Sind, Pakistan, 74800 Abbasi Shaheed Hospital Karachi, Sind, Pakistan, 74812 Malir Chest Clinic Karachi, Sind, Pakistan, 74831 Less << |
NCT02025205 | COPD Patients With Homogeneous... More >> Emphysema Endoscopic Lung Volume Reduction With Endobronchial Valve Less << | Not Applicable | Completed | - | Austria ... More >> Ludwig-Boltzmann-Institut für COPD und Pneumologische Epidemiologie Vienna, Austria, 1140 Germany Charité Campus Virchow-Klinikum Berlin, Germany, 13353 Ruhrlandklinik, Westdeutsches Lungenzentrum Essen, Germany, 45239 Lungenabteilung Thoraxzentrum Hamburg Hamburg, Germany, 21075 Thoraxklinik am Universitäts klinikum Heidelberg Heidelberg, Germany, 69126 Lungenklinik Hemer Hemer, Germany, 58675 Klinikum Nürnberg Nord Nürnberg, Germany, 90419 Netherlands Department of pulmonary dieases, University Medical Center Groningen, Netherlands, 9700RB Less << |
NCT01494896 | - | Terminated(due to slow enrollm... More >>ent. United Therapeutics and Dr. Bajwa MD decided to terminate study) Less << | - | United States, Florida ... More >> University of Florida, Jacksonville Jacksonville, Florida, United States, 32209 Mayo Clinic Jacksonville, Florida, United States, 32224 Cleveland Clinic Florida Weston, Florida, United States, 33331 Less << | |
NCT02517359 | Asthma Health... More >>y Volunteers Less << | Phase 1 | Terminated(Preset pharmacokine... More >>tic stopping criteria met at lower doses than expected.) Less << | - | United Kingdom ... More >> Manchester, United Kingdom, M23 9QZ Nottingham, United Kingdom, NG11 6JS Less << |
NCT01993914 | - | Completed | - | United States, California ... More >> The Elite Laser Vein Center Los Angeles, California, United States, 90025 Less << | |
NCT02334007 | Venous Thromboembolism ... More >> Lung Neoplasms Pulmonary Embolism Less << | Phase 1 Phase 2 | Completed | - | Canada, Ontario ... More >> St. Joseph's Healthcare Hamilton Hamilton, Ontario, Canada, L8N 4A6 Toronto General Hospital Toronto, Ontario, Canada, M5G 2C4 Less << |
NCT02663596 | Pediatric Continuous Renal Rep... More >>lacement Therapy Dialysis Renal Failure Less << | Phase 1 | Recruiting | December 2020 | United States, Pennsylvania ... More >> St. Christopher's Hospital for Children Recruiting Philadelphia, Pennsylvania, United States, 19134 Contact: Jeffrey J Cies, PharmD, MPH, BCPS-AQ ID, BCPPS 215-427-5176 jeffrey.cies@tenethealth.com Less << |
NCT01805726 | Bronchoscopy ... More >>Conscious Sedation Less << | Phase 3 | Completed | - | Lebanon ... More >> Hotel Dieu de France Hospital Beirut, Lebanon Less << |
NCT03672149 | Renal Replacement Therapy ... More >> Pharmacokinetics Less << | Phase 1 | Not yet recruiting | December 31, 2021 | - |
NCT00919152 | - | Completed | - | United States, Nebraska ... More >> The Nebraska Medical Center Omaha, Nebraska, United States, 68198 Less << | |
NCT01889758 | Kidney Transplant Recipients ... More >> Liver Transplant Recipients Less << | Phase 4 | Completed | - | United States, Ohio ... More >> The Christ Hospital Cincinnati, Ohio, United States, 45267 University of Cincinnati Cincinnati, Ohio, United States, 45267 Less << |
NCT01953081 | Enteral Feeding Intolerance | Phase 1 Phase 2 | Completed | - | Australia, South Australia ... More >> Royal Adelaide Hospital Adelaide, South Australia, Australia Less << |
NCT01953081 | - | Completed | - | - | |
NCT03466385 | Chronic Obstructive Pulmonary ... More >>Disease Respiratory Failure Acute Exacerbation Copd Less << | Not Applicable | Recruiting | December 31, 2020 | Greece ... More >> Evangelismos Hospital Recruiting Athens, Greece, 10673 Contact: Eleni Ischaki +306974856544 eischaki@yahoo.gr Less << |
NCT02685930 | - | Completed | - | United States, Missouri ... More >> Barnes-Jewish Hospital Saint Louis, Missouri, United States, 63110 Less << | |
NCT02097212 | - | Completed | - | United States, Colorado ... More >> National Jewish Health Denver, Colorado, United States, 80206 Less << | |
NCT00862862 | - | Terminated(lack of enrollment) | - | United States, Nebraska ... More >> The Nebraska Medical Center Omaha, Nebraska, United States, 68198 Less << | |
NCT02105012 | Asthma | Phase 2 | Completed | - | - |
NCT00881218 | Ischemic Heart Disease | Early Phase 1 | Completed | - | United States, Missouri ... More >> Washington University School of Medicine St. Louis, Missouri, United States, 63110 Less << |
NCT03531944 | Diabetes Mellitus, Type 2 | Not Applicable | Recruiting | May 31, 2020 | Singapore ... More >> Keat Hong Family Medicine Clinic Recruiting Singapore, Singapore, 689687 Contact: Joyce Lee Less << |
NCT02505685 | - | Active, not recruiting | July 2020 | Israel ... More >> Rabin Medical Center Petach Tikva, Israel, 49100 Less << | |
NCT02755337 | - | Unknown | December 2016 | Indonesia ... More >> Dr. Soetomo General Hospital Recruiting Surabaya, East Java, Indonesia, 60286 Contact: Laksmi Wulandari, MD. PhD +628123019591 laksmigts@yahoo.co.id Principal Investigator: Laksmi Wulandari, MD, PhD Less << | |
NCT03047941 | - | Suspended(Lack of participants... More >>) Less << | October 31, 2022 | Belgium ... More >> ASTES Bouge, Namur, Belgium, 5001 Less << | |
NCT02105012 | - | Completed | - | - | |
NCT03752437 | Extracorporeal Circulation; Co... More >>mplications Less << | Phase 4 | Not yet recruiting | March 1, 2020 | - |
NCT03574740 | - | Completed | - | - | |
NCT02682030 | Amyotrophic Lateral Sclerosis | Not Applicable | Recruiting | December 2018 | United States, California ... More >> Cedars-Sinai Medical Center Recruiting Los Angeles, California, United States, 90048 Contact: Dana Fine 310-423-1791 Less << |
NCT03102684 | Second Hand Tobacco Smoke | Not Applicable | Active, not recruiting | October 2019 | Greece ... More >> "George D Behrakis RESEARCH LAB" of the Hellenic Cancer Society (HCS) Athens, Greece, 1152 Less << |
NCT03743090 | - | Not yet recruiting | April 10, 2020 | - | |
NCT00758316 | Malignant Pleural Effusions | Phase 3 | Completed | - | Singapore ... More >> Sinagpore General Hospital Singapore, Singapore, 169608 Less << |
NCT03696524 | Pleural Effusions, Chronic ... More >> Congestive Heart Failure Shortness of Breath Less << | Not Applicable | Not yet recruiting | September 30, 2020 | - |
NCT01386203 | - | Unknown | May 2018 | Israel ... More >> Sheba Medical Center Recruiting Tel Hashomer, Israel Contact: Nir Peled peled.nir@sheba.health.gov.il Less << | |
NCT00719784 | Respiratory Diseases ... More >> Pulmonary Diseases Thoracic Diseases Lung Diseases Less << | Not Applicable | Completed | - | Singapore ... More >> Singapore General Hospital Singapore, Singapore Less << |
NCT03694847 | - | Completed | - | Canada, Quebec ... More >> Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec Québec, Quebec, Canada, G1V 4G5 Less << | |
NCT00526019 | - | Completed | - | - | |
NCT00532831 | - | Completed | - | Canada, Quebec ... More >> Centre de Recherche, Hôpital Laval Québec, Quebec, Canada, G1V 4G5 Less << | |
NCT00532363 | - | Completed | - | Canada, Quebec ... More >> Centre de Recherche, Hôpital Laval Québec, Quebec, Canada, G1V 4G5 Less << | |
NCT02338895 | - | Unknown | September 2015 | Saudi Arabia ... More >> King Faisal specialist Hospital & Research Center Recruiting Riyadh, Ar Riyadh, Saudi Arabia, 11211 Contact: Iqbal Hussain, MD 966533215378 hiqbal@kfshrc.edu.sa Contact: Nawal Salahuddin, MD,FCCP 966551948786 nsalahuddin@kfshrc.edu.sa Less << | |
NCT03118765 | Mild to Moderate Chronic Obstr... More >>uctive Pulmonary Disease (COPD) Less << | Phase 2 | Completed | - | United States, Alabama ... More >> Glenmark Investigational Site 23 Andalusia, Alabama, United States, 36420 United States, Arizona Glenmark Investigational Site 12 Phoenix, Arizona, United States, 85006 Glenmark Investigational Site 14 Tempe, Arizona, United States, 85283 United States, California Glenmark Investigational Site 17 Fullerton, California, United States, 92835 United States, Florida Glenmark Investigational Site 19 Edgewater, Florida, United States, 32132 Glenmark Investigational Site 10 Miami Lakes, Florida, United States, 33016 Glenmark Investigational Site 24 Miami, Florida, United States, 33144 Glenmark Investigational Site 16 Miami, Florida, United States, 33186 Glenmark Investigational Site 6 Orlando, Florida, United States, 32825 Glenmark Investigational Site 20 Ormond Beach, Florida, United States, 32124 Glenmark Investigational Site 21 Vero Beach, Florida, United States, 32960 Glenmark Investigational Site 13 Winter Park, Florida, United States, 32789 United States, Nevada Glenmark Investigational Site 18 Las Vegas, Nevada, United States, 89119 United States, North Carolina Glenmark Investigational Site 9 Charlotte, North Carolina, United States, 28207 United States, Ohio Glenmark Investigational Site 5 Columbus, Ohio, United States, 43213 Glenmark Investigational Site 22 Columbus, Ohio, United States, 43215 Glenmark Investigational Site 8 Dublin, Ohio, United States, 43016 United States, Oregon Glenmark Investigational Site 11 Medford, Oregon, United States, 97504-9741 United States, South Carolina Glenmark Investigational Site 3 Easley, South Carolina, United States, 29640 Glenmark Investigational Site 2 Greenville, South Carolina, United States, 29615 Glenmark Investigational Site 4 Greenville, South Carolina, United States, 29615 Glenmark Investigational Site 1 Rock Hill, South Carolina, United States, 29732 Glenmark Investigational Site 15 Spartanburg, South Carolina, United States, 29303 Glenmark Investigational Site 7 Spartanburg, South Carolina, United States, 29303 United States, Texas Glenmark Investigational Site 25 Tomball, Texas, United States, 77375 Less << |
NCT03327701 | Asthma, Exercise-Induced | Phase 3 | Not yet recruiting | April 2020 | - |
NCT00374803 | - | Completed | - | - | |
NCT02851927 | Pleural Effusion | Not Applicable | Recruiting | September 2018 | India ... More >> All India Institute of Medical Sciences Recruiting New Delhi, India, 110029 Contact: Karan Madan, MD, DM +91-11-26593676 drkaranmadan@gmail.com Less << |
NCT00374803 | End Stage Renal Disease (ESRD) | Phase 4 | Completed | - | United States, Ohio ... More >> The Christ Hospital Cincinnati, Ohio, United States, 45267 University of Cincinnati Cincinnati, Ohio, United States, 45267 Less << |
NCT00441389 | - | Completed | - | - | |
NCT02820051 | Conscious Sedation Failure Dur... More >>ing Procedure Less << | Not Applicable | Completed | - | Mexico ... More >> UANL University Hospital Monterrey, Nuevo León, Mexico, 64000 Less << |
NCT02516449 | Asthma | Not Applicable | Completed | - | Canada, Quebec ... More >> Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec Québec, Quebec, Canada, G1V 4G5 Less << |
NCT00532896 | - | Completed | - | Canada, Quebec ... More >> Centre de Recherche, Hôpital Laval Québec, Quebec, Canada, G1V 4G5 Less << | |
NCT02301923 | - | Unknown | March 2015 | Lebanon ... More >> Hotel Dieu de France Hospital Not yet recruiting Beirut, Lebanon Contact: Moussa A Riachy, MD, FCCP 961 1 615300 ext 9218 riachy@dm.net.lb Contact: Mirella Skandar, MD 96170231331 mirella.iskandar@gmail.com Sub-Investigator: Jad Choucair, MD Sub-Investigator: Samer Najem, MD Less << | |
NCT01067755 | - | Unknown | March 2011 | Germany ... More >> Thoraxklinik Recruiting Heidelberg, Germany Contact: Felix Herth Felix.Herth@thoraxklinik-heidelberg.de Principal Investigator: Felix JF Herth, MD, PhD, FCCP Less << | |
NCT02389361 | Pain | Phase 4 | Completed | - | - |
NCT00878371 | Dissecting Aneurysm of the Tho... More >>racic Aorta Less << | Phase 4 | Completed | - | Canada, Nova Scotia ... More >> Capital Health- Queen Elizabeth II Health Sciences Centre Halifax, Nova Scotia, Canada, B3H3A7 Less << |
NCT02389361 | - | Completed | - | - | |
NCT02338284 | - | Unknown | September 2015 | Saudi Arabia ... More >> King Faisal Specialist Hospital & Research Centre Recruiting Riyadh, Ar-Riyadh, Saudi Arabia, 11211 Contact: Alaa Mohamed, MD 966553730044 dr.alaibrahim@gmail.com Contact: nawal salahuddin, MD,FCCP 966551948786 nsalahuddin@kfshrc.edu.sa Sub-Investigator: Nadia AlHarbi, MD Less << | |
NCT00833300 | Delirium Agit... More >>ation Less << | Not Applicable | Terminated | - | Canada, Nova Scotia ... More >> Halifax Infirmary; Queen Elizabeth II Health Sciences Centre Halifax, Nova Scotia, Canada Victoria General Hospital; Queen Elizabeth II Health Sciences Centre Halifax, Nova Scotia, Canada Less << |
NCT02824952 | Lung Cancer | Phase 2 | Not yet recruiting | July 2020 | Israel ... More >> Rabin Medical Center Petach Tikva, Israel, 49100 Less << |
NCT03570580 | - | Recruiting | December 31, 2018 | Belgium ... More >> Clinique Saint-Luc Bouge Recruiting Namur, Belgium, 5004 Contact: Eric P Deflandre, MD, FCCP 0032477764241 eric.deflandre@gmail.com Less << | |
NCT03047421 | - | Completed | - | Belgium ... More >> Clinique Saint-Luc of Bouge Bouge, Namur, Belgium, 5004 Less << | |
NCT03047850 | - | Completed | - | Belgium ... More >> Clinique Saint-Luc of Bouge Bouge, Namur, Belgium, 5001 Less << | |
NCT03744455 | - | Recruiting | February 1, 2019 | Belgium ... More >> Clinique Saint-Luc Bouge Recruiting Namur, Belgium, 5004 Contact: Eric P Deflandre, MD, FCCP 0032477764241 eric.deflandre@gmail.com Less << | |
NCT02045121 | Pleural Effusion, Malignant | Not Applicable | Unknown | May 2015 | Singapore ... More >> Division of Respiratory and Critical Care Medicine, National University Hospital Recruiting Singapore, Singapore, 119228 Contact: Pyng Lee 65-67726533 mdclp@nus.edu.sg Principal Investigator: Pyng Lee, MBBS, MRCP, MMED, FAMS, FCCP Less << |
NCT01852838 | - | Unknown | April 2015 | Israel ... More >> Sheba Medical Center Recruiting Ramat Gan, Israel, 52621 Contact: Eve Keret 972-3-530-3207 eve.keret@sheba.health.gov.il Principal Investigator: Dr. Nir Peled, PhD,MD, FCCP Less << | |
NCT01527461 | - | Unknown | - | Israel ... More >> Sheba Medical Center Recruiting Tel Hasomer, Israel, 52621 Contact: Nir Peled, MD Phd FCCP 97235307014 nir.peled@gmail.com Contact: Michal Alumot, M.Sc,RD 97235307009 michalalumot@gmail.com Sub-Investigator: Tal Frenkel, B.Sc Less << | |
NCT01360931 | - | Unknown | June 2013 | Hong Kong ... More >> Queen Mary Hospital Recruiting Hong Kong, Hong Kong, 0 Contact: David CL Lam, MBBS, FRCP(Edin), FCCP, FACP +852 2255 5814 dcllam@hku.hk Less << | |
NCT01460108 | Pulmonary Emphysema ... More >> COPD Lung Diseases Less << | Phase 4 | Unknown | June 2013 | Austria ... More >> Otto-Wagner-Spital Recruiting Wien, Austria, 1140 Contact: Arschang Valipour, MD, FCCP 43-1-91060-41008 arschang.valipour@wienkav.at Principal Investigator: Arschang Valipour, MD, FCCP Less << |
NCT01185652 | - | Unknown | September 2012 | Hong Kong ... More >> University of Hong Kong Queen Mary Hospital Recruiting Hong Kong, Hong Kong, 0 Contact: David CL Lam, BSc,MBBS,PhD,FCCP,FACP,FRCP(E) (852) 2255 5814 dcllam@hku.hk Principal Investigator: David CL Lam, BSc,MBBS,PhD,FCCP,FACP,FRCP(E) Less << | |
NCT02049255 | Pain | Phase 4 | Completed | - | Belgium ... More >> Clinique Saint-Luc Bouge Bouge, Namur, Belgium, 5004 Less << |
NCT02051829 | - | Completed | - | Belgium ... More >> Cabinet Medical ASTES Jambes, Belgium, 5100 Less << | |
NCT01351155 | Acute Respiratory Failure | Not Applicable | Unknown | October 2015 | Italy ... More >> Pulmonary Division With Respiratory Intensive Care Unit, S. Donato Hospital, Arezzo, Italy Recruiting Lucca, Italy, 55100 Contact raffaele_scala@hotmail.com Principal Investigator: Raffaele Scala, MD, FCCP Less << |
NCT02202122 | - | Withdrawn(lack of investigator... More >>s) Less << | October 2015 | Belgium ... More >> Clinique Saint-Luc de Bouge Namur, Belgium, 5100 Less << | |
NCT02507778 | Lung Cancer | Not Applicable | Recruiting | September 2019 | Israel ... More >> Rabin Medical Center Recruiting Petach Tikva, Israel, 49100 Contact: Nir Peled, MD PhD FCCP nirp@clalit.org.il Less << |
NCT02198820 | - | Withdrawn(lack of investigator... More >>s) Less << | September 2015 | Belgium ... More >> Clinique Saint-Luc de Bouge Namur, Belgium, 5004 Less << | |
NCT02324946 | - | Completed | - | Belgium ... More >> Clinique Saint-Luc de Bouge Bouge, Namur, Belgium, 5004 Less << | |
NCT02282280 | - | Completed | - | Belgium ... More >> Clinique Saint-Luc Bouge Namur, Belgium, 5004 Less << | |
NCT02452645 | Family Child Care Homes | Not Applicable | Recruiting | May 2019 | United States, Rhode Island ... More >> Ready 2 Learn Recruiting Providence, Rhode Island, United States, 02903 Contact: Leslie Gell Less << |
NCT02055027 | - | Completed | - | Belgium ... More >> Centre Hospitalier Universitaire (CHU) Sart-Tilman Liege, Belgium, 4000 Less << | |
NCT00103766 | Empyema Pleur... More >>al Effusion Less << | Not Applicable | Unknown | - | United States, Texas ... More >> Scott and White Memorial Hospital & Clinic Recruiting Temple, Texas, United States, 76508 Contact: Delores Gautier, RN, MSN 254-724-1769 lgautier@swmail.sw.org Principal Investigator: Jeana D O'Brien, MD, FACP, FCCP Sub-Investigator: Dominic R deKeratry, MD Sub-Investigator: Timothy S Mooring, MD Less << |
NCT03172806 | - | Completed | - | Belgium ... More >> Clinique Saint-Luc de Bouge Bouge, Namur, Belgium, 5004 Less << | |
NCT03309280 | - | Recruiting | October 31, 2019 | Belgium ... More >> Clinique Saint-Luc de Bouge Recruiting Bouge, Namur, Belgium, 5004 Contact: Eric DEFLANDRE, MD, FCCP 0032477764241 eric.deflandre@gmail.com Less << | |
NCT02736513 | Lung Cancer | Phase 2 | Recruiting | May 2020 | Israel ... More >> Rabin Medical Center Recruiting Petach Tikva, Israel, 49100 Contact: Nir Peled, MD PhD FCCP nirp@clalit.org.il Less << |
NCT03219866 | COPD COPD Exa... More >>cerbation Less << | Phase 4 | Recruiting | September 2019 | United States, North Carolina ... More >> Wake Forest Baptist Health Recruiting Winston-Salem, North Carolina, United States, 27104 Contact: Jill A Ohar, MD, FCCP 336-716-1210 johar@wakehealth.edu Less << |
NCT02050685 | - | Completed | - | Belgium ... More >> Clinique Saint-Luc de Bouge Bouge, Namur, Belgium, 5004 Less << | |
NCT02539264 | - | Completed | - | - | |
NCT02973139 | Pleural Infection ... More >> Pleural Diseases Less << | Not Applicable | Recruiting | November 2019 | United States, Massachusetts ... More >> Beth Israel Deaconess Medical Center Recruiting Boston, Massachusetts, United States, 02215 Contact: Adnan Majid, MD FCCP 617-632-8252 amajid@bidmc.harvard.edu Principal Investigator: Adnan Majid, MD FCCP Less << |
Bio Calculators | ||||
Preparing Stock Solutions | ![]() |
1mg | 5mg | 10mg |
1 mM 5 mM 10 mM |
3.93mL 0.79mL 0.39mL |
19.67mL 3.93mL 1.97mL |
39.34mL 7.87mL 3.93mL |
|
Dissolving Methods |
in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day; The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound. Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:
|