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Chemical Structure| 98181-63-6 Chemical Structure| 98181-63-6
Chemical Structure| 98181-63-6

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5(6)-TAMRA contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. It is a bright, orange-fluorescent dye that produces conjugates with absorption/emission maxima of ~555/580 nm.

Synonyms: 5(6)-Carboxytetramethylrhodamine

4.5 *For Research Use Only !

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Product Citations

Product Citations

Choi, So Ra ; Wang, Hee Myeong ; Shin, Min Hyeon ; Lim, Hyun-Suk ;

Abstract: Steroid receptor coactivator-1 (SRC-1) is a transcription coactivator playing a pivotal role in mediating a wide range of signaling pathways by interacting with related transcription factors and nuclear receptors. Aberrantly elevated SRC-1 activity is associated with cancer metastasis and progression, and therefore, suppression of SRC-1 is emerging as a promising therapeutic strategy. In this study, we developed a novel SRC-1 degrader for targeted degradation of cellular SRC-1. This mol. consists of a selective ligand for SRC-1 and a bulky hydrophobic group. Since the hydrophobic moiety on the protein surface could mimic a partially denatured hydrophobic region of a protein, SRC-1 could be recognized as an unfolded protein and experience the chaperone-mediated degradation in the cells through the ubiquitin-proteasome system (UPS). Our results demonstrate that a hydrophobic-tagged chimeric mol. is shown to significantly reduce cellular levels of SRC-1 and suppress cancer cell migration and invasion. Together, these results highlight that our SRC-1 degrader represents a novel class of therapeutic candidates for targeting cancer metastasis. Moreover, we believe that the hydrophobic tagging strategy would be widely applicable to develop peptide-based protein degraders with enhanced cellular activity.

Keywords: PROTACs ; SRC-1 transcriptional coactivator ; cancer metastasis ; hydrophobic tagging ; proteasomal degradation ; ubiquitination ; ubiquitin–proteasome system

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Product Details of 5(6)-TAMRA

CAS No. :98181-63-6
Formula : C25H22N2O5
M.W : 430.45
SMILES Code : O=C(C1=CC=C(C=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)C(O)=O)[O-].O=C(C5=CC=C(C(C(O)=O)=C5)C6=C7C=CC(N(C)C)=CC7=[O+]C8=C6C=CC(N(C)C)=C8)[O-]
Synonyms :
5(6)-Carboxytetramethylrhodamine
MDL No. :MFCD00467499

Safety of 5(6)-TAMRA

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P305+P351+P338
 

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