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With sodium methylate; In methanol; at 20.0℃; for 1.5h;pH 9.0;
1-n-nonyl-2,3,4,6-tetraacetyl-beta-D-glucopyranoside was dissolved in methanol,Sodium methoxide was added to adjust the pH to 9, the reaction was carried out for 1.5 hours at room temperature, neutralized with strong acid cation exchange resin HCRW-20, filtered, the filtrate was evaporated to dryness,The result is n-nonyl-beta-D-glucopyranoside.The yield is 54%. And products in beta configuration> 97%
With almond meal; In water; at 50.0℃; for 168.0h;Thermodynamic data; Equilibrium constant;
General procedure: For the hydrolytic reaction of glucosides, 0.25 mol/L of propyl, hexyl, or octyl glucoside was dissolved in a mixture of18 ml of the corresponding alcohol and 2 ml of water containing60 mg almond meal. The reaction was performed in a100-ml flask that was constantly shaken at 180 r/min at 50 C.After the mixture was incubated for 72 and 144 h respectively,30 mg almond meal was supplemented. The reverse hydrolysisof the propyl, hexyl, and octyl glucosides was performed as inthe n-alkyl -D-glucosides synthesis.
With almond meal; In water; at 50.0℃; for 168.0h;Enzymatic reaction;Thermodynamic data; Equilibrium constant;
General procedure: In a typical experiment, an alkyl alcohol was used as thesubstrate and the medium. The reaction mixture was composedof 18 ml of primary alcohol and 2 ml of water containing 0.25mol/L glucose (Scheme 1). After 60 mg almond meal wasadded, the mixture was incubated in a 100-ml flask and constantlyshaken at 180 r/min at 50 C. At certain intervals, 50 lof the mixture was withdrawn and analyzed by HPLC.For the synthesis of methyl -D-glucoside, 2 ml of methanol,16 ml of acetonitrile, and 2 ml of water containing 0.25 mol/Lglucose were mixed.