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Chemical Structure| 7240-90-6 Chemical Structure| 7240-90-6

Structure of X-GAL
CAS No.: 7240-90-6

Chemical Structure| 7240-90-6

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X-GAL is an organic compound consisting of galactose linked to a substituted indole and is much used in molecular biology to test for the presence of an enzyme, β-galactosidase.

Synonyms: BCIG; 5-bromo-4-chloro-3-indolyl-β-D-Galactopyranoside

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Product Details of X-GAL

CAS No. :7240-90-6
Formula : C14H15BrClNO6
M.W : 408.63
SMILES Code : O[C@H]1[C@H](OC2=CNC3=C2C(Cl)=C(Br)C=C3)O[C@H](CO)[C@H](O)[C@@H]1O
Synonyms :
BCIG; 5-bromo-4-chloro-3-indolyl-β-D-Galactopyranoside
MDL No. :MFCD00005666
InChI Key :OPIFSICVWOWJMJ-AEOCFKNESA-N
Pubchem ID :65181

Safety of X-GAL

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P305+P351+P338

Related Pathways of X-GAL

MAPK

Isoform Comparison

Biological Activity

Description
X-GAL (BCIG) is a widely recognized chromogenic substrate for β-galactosidase, remaining colorless until enzymatic cleavage occurs. This reaction transforms X-GAL into an insoluble blue compound, allowing for easy detection. Such properties make X-GAL especially valuable in applications like blue-white screening for cloning in bacteria and assessing β-galactosidase activity across various research contexts[1].[2].

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

2.45mL

0.49mL

0.24mL

12.24mL

2.45mL

1.22mL

24.47mL

4.89mL

2.45mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2

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