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Chemical Structure| 7296-64-2 Chemical Structure| 7296-64-2

Structure of 7296-64-2

Chemical Structure| 7296-64-2

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Product Details of [ 7296-64-2 ]

CAS No. :7296-64-2
Formula : C6H12O6
M.W : 180.16
SMILES Code : O[C@H]1[C@H](O)O[C@H](CO)[C@H](O)[C@@H]1O
MDL No. :MFCD00871336

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Application In Synthesis of [ 7296-64-2 ]

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

  • Downstream synthetic route of [ 7296-64-2 ]

[ 7296-64-2 ] Synthesis Path-Downstream   1~1

  • 1
  • [ 7493-95-0 ]
  • [ 3646-73-9 ]
  • [ 100-02-7 ]
  • [ 7296-64-2 ]
  • [ 110891-71-9 ]
  • 4-nitrophenyl (α-D-galactopyranosyl)-(1->2)-α-D-galactopyranoside [ No CAS ]
  • [ 187394-28-1 ]
YieldReaction ConditionsOperation in experiment
With glycoside hydrolase family 36 alpha-galactosidase from Thermotoga maritima; In aq. phosphate buffer; at 60.0℃;pH 7.0;Kinetics; All pNP-galactobiosides used were synthesized via transglycosylationfrom pNPGal catalysed by TmGal36A. The transglycosylationreaction was carried out at 60 C in 50 mM sodium phosphatebuffer, pH 7.0. The reaction mixture (3 mL) contained 180 mg(200 mM) of pNPGal. The reaction was initiated by the addition of18 U of the enzyme. The progress of the reaction was monitoredby thin layer chromatography as described above. The reactionwas stopped by incubating the sample for 10 min in boiling water.To determine the substrate conversion, 5 lL aliquots of the reactionmixture were withdrawn, 10% Na2CO3 (3 mL) was added and the absorbance at 400 nm in 1 cm path-length cuvette was measuredwith a Jasco V-560 spectrophotometer.For separation of the transglycosylation products, the reactionmixture was applied to a Discovery BIO Wide Pore C18 column(250 21.2 mm, 10 lm, Supelco) and then eluted with a lineargradient (0-90%) of acetonitrile in water for 180 min at a flow rateof 4 mL/min; the eluent was monitored by absorption at 303 nm.Fractions containing products of transglycosylation were freezedried,and compounds were identified by NMR analysis. 1H NMR,1H-13C HSQC and 1H-1H NOESY measurements were made to identifychemical structures of the pNP galactobiosides obtained. Thespectra obtained were in a good agreement with the publisheddata.21
 

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