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P21583-Human SCF-3d structure.jpg P21583-Human SCF-3d structure.jpg

Human SCF+Cell Based Assay.jpg Human SCF+Cell Based Assay.jpg Induces proliferation in TF-1 cells. ED50: 1-5 ng/ml.

Induces proliferation in TF-1 cells. ED50: 1-5 ng/ml.

Human SCF+SDS-PAGE.jpg Human SCF+SDS-PAGE.jpg Greater than 95% as determined by reducing SDS-PAGE.

Greater than 95% as determined by reducing SDS-PAGE.

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Recombinant Human SCF is a human-derived protein expressed in E. coli as an untagged form. This product, also known as stem cell factor (SCF), is a hematopoietic growth factor that plays a critical role in early hematopoiesis. It stimulates the proliferation of myeloid, erythroid, and lymphoid progenitor cells in bone marrow cultures and exhibits synergistic effects with colony-stimulating factors.

Synonyms: Recombinant Human SCF; Kit Ligand; Mast Cell Growth Factor

4.5 *For Research Use Only !

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Product Details of Human SCF

M.W : 18.6 KDa
SMILES Code : NONE
Synonyms :
Recombinant Human SCF; Kit Ligand; Mast Cell Growth Factor
MDL No. :N/A

Safety of Human SCF

Isoform Comparison

Biological Activity

Description
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.
Protein Species Human
Expression System E.coli
Tag Tag free
Protein Expression Interval Glu26-Ala189
Protein ID P21583
Endotoxin < 0.01 EU/µg as determined by LAL test.
Characteristics Lyophilized powder
Formulation Description Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.0.
Reconstitution Method Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
For long-term storage, it is recommended to dilute the product further with a solution containing carrier proteins (e.g., 0.1% BSA, 10% FBS, or 5% HSA). For serum-free cultures or in vivo animal experiments where BSA, FBS, or HSA cannot be present in the cytokine preparation, trehalose may be used as an alternative carrier. Aliquots should be stored frozen at -20°C to -80°C.
Note: Low-adsorption tubes are recommended for aliquot storage, and repeated freeze-thaw cycles should be avoided. The dilution concentration for aliquoting should not be lower than 10 μg/mL, and the aliquot volume should be no less than 10 μL.
Aliquoted protein remains stable for up to 3 months when stored at ≤ -20°C.
 

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