CAS No. :	1043444-18-3
Formula :	C₁₆H₂₀ClF₃N₄O₄
M.W :	424.80
SMILES Code :	O=C(N)[C@@H](NC(C1=CC=CC=C1)=O)CCCNC(CCl)=N.O=C(O)C(F)(F)F
Synonyms :	Cl-amidine
MDL No. :	MFCD31536783
InChI Key :	WUSNMVYWOLUWDD-MERQFXBCSA-N
Pubchem ID :	57402549

GHS Pictogram:
Signal Word:	Warning
Hazard Statements:	H302-H315-H319-H335
Precautionary Statements:	P261-P280-P301+P312-P302+P352-P305+P351+P338

CD68+VSMCs	100 µM	0.5 hours	Inhibit PAD4 activity, reduce ETs generation	Nat Commun. 2022 Dec 6;13(1):7500.
HEK293T/PAD2 cells	25 µM	1 hour	To evaluate the target engagement of Cl-amidine TFA in HEK293T/PAD2 cells, results showed that it effectively entered the cells and covalently modified PAD2 at 25 μM concentration.	J Med Chem. 2017 Apr 13;60(7):3198-3211.
NZM mouse neutrophils	200 µM	12 hours	Cl-amidine inhibited NET release from NZM mouse neutrophils	J Clin Invest. 2013 Jul;123(7):2981-93.
BALB/c mouse neutrophils	200 µM	12 hours	Cl-amidine inhibited NZM serum-induced NET release from BALB/c mouse neutrophils	J Clin Invest. 2013 Jul;123(7):2981-93.
Keratinocytes	0–800 µM	2 days	Cl-amidine inhibited deimination in a dose-dependent manner and was not cytotoxic for keratinocytes. At 800 μM, Cl-amidine was shown to reduce deimination by half, alter keratinocyte differentiation, decrease the number of corneocyte layers, significantly increase the number of transitional cells, induce clustering of mitochondria and of heterogeneous vesicles in the cytoplasm of granular keratinocytes, and upregulate the expression of autophagy proteins, including LC3-II, sestrin-2 and p62/SQSTM1.	J Invest Dermatol. 2019 Sep;139(9):1889-1897.e4.
Human brain microvascular endothelial cells (HBMVECs)	6 µM	24 hours	To assess the direct effect of Cl-amidine on brain endothelial cell PADs, results showed no significant effect of Cl-amidine treatment on vWF release, indicating no citrullination in brain endothelial cells.	Nat Cardiovasc Res. 2024 Dec;3(12):1549-1567.
Human brain microvascular endothelial cells (HBMVECs)	500 ng/mL	24-72 hours	To assess the effect of NETs on EndMT in brain endothelial cells, results showed that NETs promote EndMT, as evidenced by changes in cell morphology and increased Snail expression.	Nat Cardiovasc Res. 2024 Dec;3(12):1549-1567.
MCF7/TamR cells	200 µM	4 days	To evaluate the inhibitory effect of Cl-amidine on the proliferation of MCF7/TamR cells, results showed that 200 μM Cl-amidine significantly inhibited cell proliferation	J Exp Clin Cancer Res. 2019 Oct 10;38(1):414.
MCF7/TamR cells	50 µM	4 days	To evaluate the inhibitory effect of Cl-amidine combined with tamoxifen on the proliferation of MCF7/TamR cells, results showed that 50 μM Cl-amidine combined with 5 μM tamoxifen significantly inhibited cell proliferation	J Exp Clin Cancer Res. 2019 Oct 10;38(1):414.
MCF7/TamR cells	25 µM	4 days	To evaluate the effect of Cl-amidine combined with docetaxel on the viability of MCF7/TamR cells, results showed that 0.1 μM docetaxel combined with 25 μM Cl-amidine significantly decreased cell viability	J Exp Clin Cancer Res. 2019 Oct 10;38(1):414.
U2OS cells	20 µM	72 hours	To evaluate the antiproliferative activities of Cl-amidine TFA analogs on U2OS cells. Results showed that biphenyl tetrazole tert-butyl Cl-amidine (13) completely abolished the growth of U2OS cells at 20 μM.	J Med Chem. 2015 Feb 12;58(3):1337-44.

C57BL/6 mice	Traumatic brain injury model	Intraperitoneal injection	10 mg/kg	Once daily for three consecutive days	Inhibition of PAD4 significantly ameliorated neurological deficits in mice and reduced neuronal pyroptosis by inhibiting the STING/IRE1α-NLRP1 pathway	Front Immunol. 2023 Apr 14;14:1125759
NZM mice	NZM mouse lupus model	Subcutaneous injection	10 mg/kg/d	Daily for 14 weeks	Cl-amidine significantly improved vasculogenesis, endothelial function, and prothrombotic phenotype in NZM mice	J Clin Invest. 2013 Jul;123(7):2981-93.
Mice	Ccm3-iECKO mice	Intraperitoneal injection	10 mg/kg/day	Once daily for 7 days	To assess the effect of Cl-amidine on NET formation and CCM lesions, results showed that Cl-amidine significantly reduced NET formation and lesion burden, improving endothelial activation, EndMT, coagulation, and neuroinflammation.	Nat Cardiovasc Res. 2024 Dec;3(12):1549-1567.
Pad4−/− mice	Citrobacter rodentium infection model	Intraperitoneal injection	20 mg/kg	Administered 3 hours prior to infection, followed by once daily for the next 3 consecutive days and then once every alternate day until day 11	Cl-amidine treatment modestly enhanced the susceptibility of WT mice to C. rodentium infection, as evidenced by increased CR burden in feces and worsened colonic histopathology, though to a lesser extent compared to Pad4?/? mice and DNase I-treated mice.	Mucosal Immunol. 2019 May;12(3):761-771
BALB/c Nude mice	Breast cancer xenograft model	Intraperitoneal injection	20 mg/kg/day	Every 3 days for 3 weeks	To evaluate the effect of Cl-amidine combined with docetaxel on tumor growth in a breast cancer xenograft model, results showed that the combination treatment significantly reduced tumor weight	J Exp Clin Cancer Res. 2019 Oct 10;38(1):414.
C57BL/6J mice	DSS-induced colitis model	Gavage	25mg/kg	Once daily for 7 days	To evaluate the effect of Cl-amidine on DSS-induced colitis, the results showed that Cl-amidine alleviated the severity of colitis, reduced weight loss, decreased the disease activity index (DAI) score, and increased colon length.	Front Immunol. 2023 Apr 18;14:1144976
C57BL/6 mice	Traumatic brain injury (TBI) model	Intraperitoneal injection	50 mg/kg	Once daily for three consecutive days	Cl-amidine treatment improved short-term neurological functions, reduced cerebral lesion volume, reduced brain edema, and restored cerebral blood flow (CBF) after TBI. In addition, Cl-amidine exerted neuroprotective effects by attenuating BBB disruption, inhibiting immune cell infiltration, and alleviating neuronal death after TBI.	J Neuroinflammation. 2023 Sep 30;20(1):222

Precautionary Statements-General
Code	Phrase
P101	If medical advice is needed,have product container or label at hand.
P102	Keep out of reach of children.
P103	Read label before use
Prevention
Code	Phrase
P201	Obtain special instructions before use.
P202	Do not handle until all safety precautions have been read and understood.
P210	Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
P211	Do not spray on an open flame or other ignition source.
P220	Keep/Store away from clothing/combustible materials.
P221	Take any precaution to avoid mixing with combustibles
P222	Do not allow contact with air.
P223	Keep away from any possible contact with water, because of violent reaction and possible flash fire.
P230	Keep wetted
P231	Handle under inert gas.
P232	Protect from moisture.
P233	Keep container tightly closed.
P234	Keep only in original container.
P235	Keep cool
P240	Ground/bond container and receiving equipment.
P241	Use explosion-proof electrical/ventilating/lighting/equipment.
P242	Use only non-sparking tools.
P243	Take precautionary measures against static discharge.
P244	Keep reduction valves free from grease and oil.
P250	Do not subject to grinding/shock/friction.
P251	Pressurized container: Do not pierce or burn, even after use.
P260	Do not breathe dust/fume/gas/mist/vapours/spray.
P261	Avoid breathing dust/fume/gas/mist/vapours/spray.
P262	Do not get in eyes, on skin, or on clothing.
P263	Avoid contact during pregnancy/while nursing.
P264	Wash hands thoroughly after handling.
P265	Wash skin thouroughly after handling.
P270	Do not eat, drink or smoke when using this product.
P271	Use only outdoors or in a well-ventilated area.
P272	Contaminated work clothing should not be allowed out of the workplace.
P273	Avoid release to the environment.
P280	Wear protective gloves/protective clothing/eye protection/face protection.
P281	Use personal protective equipment as required.
P282	Wear cold insulating gloves/face shield/eye protection.
P283	Wear fire/flame resistant/retardant clothing.
P284	Wear respiratory protection.
P285	In case of inadequate ventilation wear respiratory protection.
P231 + P232	Handle under inert gas. Protect from moisture.
P235 + P410	Keep cool. Protect from sunlight.
Response
Code	Phrase
P301	IF SWALLOWED:
P304	IF INHALED:
P305	IF IN EYES:
P306	IF ON CLOTHING:
P307	IF exposed:
P308	IF exposed or concerned:
P309	IF exposed or if you feel unwell:
P310	Immediately call a POISON CENTER or doctor/physician.
P311	Call a POISON CENTER or doctor/physician.
P312	Call a POISON CENTER or doctor/physician if you feel unwell.
P313	Get medical advice/attention.
P314	Get medical advice/attention if you feel unwell.
P315	Get immediate medical advice/attention.
P320
P302 + P352	IF ON SKIN: wash with plenty of soap and water.
P321
P322
P330	Rinse mouth.
P331	Do NOT induce vomiting.
P332	IF SKIN irritation occurs:
P333	If skin irritation or rash occurs:
P334	Immerse in cool water/wrap n wet bandages.
P335	Brush off loose particles from skin.
P336	Thaw frosted parts with lukewarm water. Do not rub affected area.
P337	If eye irritation persists:
P338	Remove contact lenses, if present and easy to do. Continue rinsing.
P340	Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P341	If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P342	If experiencing respiratory symptoms:
P350	Gently wash with plenty of soap and water.
P351	Rinse cautiously with water for several minutes.
P352	Wash with plenty of soap and water.
P353	Rinse skin with water/shower.
P360	Rinse immediately contaminated clothing and skin with plenty of water before removing clothes.
P361	Remove/Take off immediately all contaminated clothing.
P362	Take off contaminated clothing and wash before reuse.
P363	Wash contaminated clothing before reuse.
P370	In case of fire:
P371	In case of major fire and large quantities:
P372	Explosion risk in case of fire.
P373	DO NOT fight fire when fire reaches explosives.
P374	Fight fire with normal precautions from a reasonable distance.
P376	Stop leak if safe to do so. Oxidising gases (section 2.4) 1
P377	Leaking gas fire: Do not extinguish, unless leak can be stopped safely.
P378
P380	Evacuate area.
P381	Eliminate all ignition sources if safe to do so.
P390	Absorb spillage to prevent material damage.
P391	Collect spillage. Hazardous to the aquatic environment
P301 + P310	IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P301 + P312	IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
P301 + P330 + P331	IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
P302 + P334	IF ON SKIN: Immerse in cool water/wrap in wet bandages.
P302 + P350	IF ON SKIN: Gently wash with plenty of soap and water.
P303 + P361 + P353	IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P304 + P312	IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell.
P304 + P340	IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P304 + P341	IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338	IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P306 + P360	IF ON CLOTHING: Rinse Immediately contaminated CLOTHING and SKIN with plenty of water before removing clothes.
P307 + P311	IF exposed: call a POISON CENTER or doctor/physician.
P308 + P313	IF exposed or concerned: Get medical advice/attention.
P309 + P311	IF exposed or if you feel unwell: call a POISON CENTER or doctor/physician.
P332 + P313	IF SKIN irritation occurs: Get medical advice/attention.
P333 + P313	IF SKIN irritation or rash occurs: Get medical advice/attention.
P335 + P334	Brush off loose particles from skin. Immerse in cool water/wrap in wet bandages.
P337 + P313	IF eye irritation persists: Get medical advice/attention.
P342 + P311	IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P370 + P376	In case of fire: Stop leak if safe to Do so.
P370 + P378	In case of fire:
P370 + P380	In case of fire: Evacuate area.
P370 + P380 + P375	In case of fire: Evacuate area. Fight fire remotely due to the risk of explosion.
P371 + P380 + P375	In case of major fire and large quantities: Evacuate area. Fight fire remotely due to the risk of explosion.
Storage
Code	Phrase
P401
P402	Store in a dry place.
P403	Store in a well-ventilated place.
P404	Store in a closed container.
P405	Store locked up.
P406	Store in corrosive resistant/ container with a resistant inner liner.
P407	Maintain air gap between stacks/pallets.
P410	Protect from sunlight.
P411
P412	Do not expose to temperatures exceeding 50 oC/ 122 oF.
P413
P420	Store away from other materials.
P422
P402 + P404	Store in a dry place. Store in a closed container.
P403 + P233	Store in a well-ventilated place. Keep container tightly closed.
P403 + P235	Store in a well-ventilated place. Keep cool.
P410 + P403	Protect from sunlight. Store in a well-ventilated place.
P410 + P412	Protect from sunlight. Do not expose to temperatures exceeding 50 oC/122oF.
P411 + P235	Keep cool.
Disposal
Code	Phrase
P501	Dispose of contents/container to ...
P502	Refer to manufacturer/supplier for information on recovery/recycling

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CAS No.: 1043444-18-3

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CAS No.: 1043444-18-3

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Product Details of Cl-amidine TFA

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Physical hazards
Code	Phrase
H200	Unstable explosive
H201	Explosive; mass explosion hazard
H202	Explosive; severe projection hazard
H203	Explosive; fire, blast or projection hazard
H204	Fire or projection hazard
H205	May mass explode in fire
H220	Extremely flammable gas
H221	Flammable gas
H222	Extremely flammable aerosol
H223	Flammable aerosol
H224	Extremely flammable liquid and vapour
H225	Highly flammable liquid and vapour
H226	Flammable liquid and vapour
H227	Combustible liquid
H228	Flammable solid
H229	Pressurized container: may burst if heated
H230	May react explosively even in the absence of air
H231	May react explosively even in the absence of air at elevated pressure and/or temperature
H240	Heating may cause an explosion
H241	Heating may cause a fire or explosion
H242	Heating may cause a fire
H250	Catches fire spontaneously if exposed to air
H251	Self-heating; may catch fire
H252	Self-heating in large quantities; may catch fire
H260	In contact with water releases flammable gases which may ignite spontaneously
H261	In contact with water releases flammable gas
H270	May cause or intensify fire; oxidizer
H271	May cause fire or explosion; strong oxidizer
H272	May intensify fire; oxidizer
H280	Contains gas under pressure; may explode if heated
H281	Contains refrigerated gas; may cause cryogenic burns or injury
H290	May be corrosive to metals
Health hazards
Code	Phrase
H300	Fatal if swallowed
H301	Toxic if swallowed
H302	Harmful if swallowed
H303	May be harmful if swallowed