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Chemical Structure| 23491-55-6 Chemical Structure| 23491-55-6

Structure of Hoechst 33258 analog 5
CAS No.: 23491-55-6

Chemical Structure| 23491-55-6

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Hoechst 33258 analog 5 is a member of Hoechst stains, part of a family of blue fluorescent dyes used to stain DNA.

4.5 *For Research Use Only !

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Product Details of Hoechst 33258 analog 5

CAS No. :23491-55-6
Formula : C29H26N6
M.W : 458.56
SMILES Code : CN1CCN(C2=CC=C3N=C(C4=CC=C5N=C(C6=CC=C7C=CC=CC7=C6)NC5=C4)NC3=C2)CC1
MDL No. :MFCD25976772
InChI Key :FJXFKDPHPUCTJZ-UHFFFAOYSA-N
Pubchem ID :10321921

Safety of Hoechst 33258 analog 5

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302
Precautionary Statements:P280-P305+P351+P338

Isoform Comparison

Biological Activity

Description
Hoechst 33258 analog 5 is a nuclear marker dye in Hoechst series, primarily binding to the A/T-rich regions of DNA strands. Its binding affinity is not restricted to DNA, but the fluorescence intensity is significantly enhanced when bound to A/T-rich double-stranded DNA, making it a valuable tool for live cell labeling. The fluorescence intensity of Hoechst dye increases with the solution's pH, enabling its application across a range of biological research fields[1].

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

2.18mL

0.44mL

0.22mL

10.90mL

2.18mL

1.09mL

21.81mL

4.36mL

2.18mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2

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