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Chemical Structure| 10597-60-1 Chemical Structure| 10597-60-1

Structure of Hydroxytyrosol
CAS No.: 10597-60-1

Chemical Structure| 10597-60-1

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2-(3,4-Dihydroxyphenyl)ethanol is a potent and specific inhibitor of lipoxygenase activities, inhibits platelet 12-LO activity with IC50 of 4.2 mM and PMNL 5-LO activity with IC50 of 13 mM. It's a natural product isolated and purified from the herbs of Canarium album with antioxidant property.

Synonyms: DOPET; 3,4-Dihydroxyphenethyl alcohol; 3Hydroxytyrosol3 4dihydroxyphenylethanol (DOPET)Dihydroxyphenylethanol 2(34Dihydroxyphenyl)ethanol (DHPE)34dihydroxyphenolethanol (34DHPEA)

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Product Details of Hydroxytyrosol

CAS No. :10597-60-1
Formula : C8H10O3
M.W : 154.16
SMILES Code : OCCC1=CC=C(O)C(O)=C1
Synonyms :
DOPET; 3,4-Dihydroxyphenethyl alcohol; 3Hydroxytyrosol3 4dihydroxyphenylethanol (DOPET)Dihydroxyphenylethanol 2(34Dihydroxyphenyl)ethanol (DHPE)34dihydroxyphenolethanol (34DHPEA)
MDL No. :MFCD01320529
InChI Key :JUUBCHWRXWPFFH-UHFFFAOYSA-N
Pubchem ID :82755

Safety of Hydroxytyrosol

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H315-H319-H335
Precautionary Statements:P261-P305+P351+P338

Isoform Comparison

Biological Activity

In Vitro:

Cell Line
Concentration Treated Time Description References
MDA-MB-231 cells 0 to 6 μg/mL 24, 48, and 72 h Evaluate the antiproliferative effects of hydroxytyrosol nanocapsules on MDA-MB-231 cells. Results showed that the IC50 values of free hydroxytyrosol increased from 107.17 μM to 183.65 μM, while nanoencapsulated hydroxytyrosol exhibited higher antiproliferative activity. The IC50 value for HTS2 was significantly higher than HTS1 throughout the 72 HTP. PMC8799616
A549 cells 0 to 6 μg/mL 24, 48, and 72 h Evaluate the antiproliferative effects of hydroxytyrosol nanocapsules on A549 cells. Results showed that nanoencapsulated hydroxytyrosol had significantly higher antiproliferative activity than free hydroxytyrosol. The IC50 values for HTS1 decreased from 199.18 μM to 60.74 μM, and for HTS2 from 125.28 μM to 56.31 μM. PMC8799616
Jurkat cells 50 μM and 100 μM 30 min To investigate the protective effect of hydroxytyrosol against H2O2-induced DNA damage, results showed that hydroxytyrosol significantly reduced DNA damage. PMC5094689
Zebrafish liver cell line (ZFL) 50 μM 24 h To evaluate the effects of hydroxytyrosol on fat deposition and mitochondrial function. Results showed that hydroxytyrosol reduced fat deposition and improved mitochondrial function. PMC9138034
U2OS osteosarcoma cells 50 μM 48 h To assess whether HT affects the antitumor activity of Dox, results showed that HT did not interfere with the antineoplastic properties of Dox in osteosarcoma cells. PMC9220035
H9c2 cardiomyoblasts 50 μM 24 and 48 h To evaluate the protective effect of HT against Dox-induced cytotoxicity, results showed that HT significantly reduced Dox-induced cell viability decline and morphological changes. PMC9220035
BV2 cells 1, 10, 25, 50 μM 6 h To evaluate the inhibitory effect of HT on LPS- and α-syn-induced inflammatory responses in BV2 cells. Results showed HT significantly reduced mRNA levels of pro-inflammatory mediators such as TNF-α, iNOS, IL-1β, IL-6, and CXCL10. PMC7022576
SH-SY5Y human neuroblastoma cells 0.5 mM、1 mM、2 mM 7 days To test the effect of Hydroxytyrosol on non-enzymatic glycation in human insulin. The results showed that Hydroxytyrosol significantly inhibited the glycation of insulin by D-ribose, reducing the formation of AGEs in a concentration-dependent manner PMC8301023
Bovine mammary epithelial cells (MAC-T cells) 10 μM and 25 μM 1 h and 6 h To evaluate the protective effect of hydroxytyrosol against LPS-induced oxidative stress and inflammatory response. Results showed that hydroxytyrosol reduced intracellular ROS levels, increased GSH levels, and downregulated NOX-1 mRNA expression. PMC7464001

Clinical Trial:

NCT Number Conditions Phases Recruitment Completion Date Locations
NCT02068092 Breast Cancer PHASE2|PHASE3 COMPLETED 2025-07-21 Houston Methodist Hospital, Ho... More >>uston, Texas, 77030, United States|Houston Methodist Hospital Willowbrook, Houston, Texas, 77070, United States|Houston Methodist Hospital Sugar Land, Sugar Land, Texas, 77479, United States Less <<

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

6.49mL

1.30mL

0.65mL

32.43mL

6.49mL

3.24mL

64.87mL

12.97mL

6.49mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2

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