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Chemical Structure| 134-03-2 Chemical Structure| 134-03-2

Structure of L-Ascorbic acid sodium salt
CAS No.: 134-03-2

Chemical Structure| 134-03-2

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Sodium ascorbate is a more bioavailable form of vitamin C that is an alternative to taking ascorbic acid as a supplement.

Synonyms: Sodium ascorbate; Sodium L-ascorbate; Sodium Vitamin C

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Product Citations

Product Citations

Ang, Charles G ; Hyatt, Nadia L ; Le Minh, Giang ; Gupta, Monisha ; Kadam, Manali ; Hogg, Philip J , et al.

Abstract: Disulfide exchange is underexplored as a mechanism influencing HIV-1 entry. Prior studies demonstrated that redox enzyme inhibition can prevent HIV-1 infection but with limited mechanistic explanation. We hypothesize that ligand-driven rearrangement (“conformational activation”) enables enzyme-mediated disulfide exchange in Env residues (“disulfide trigger”) that promotes fusion transformations, enhancing virus entry. We tested soluble CD4 and CD4-binding site entry inhibitors as conformational activators and the ubiquitous redox enzyme thioredoxin-1 (Trx1) as disulfide trigger. We found that combination treatment caused fusion-like Env transformation and pseudovirus lysis, independent of cells. Notably, only compounds associated with gp120 shedding caused lysis when paired with Trx1. In each case, lysis was prevented by adding the fusion inhibitor T20, demonstrating that six-helix bundle formation is required as in virus-cell fusion. In contrast to conformationally activating ligands, neither the ground state stabilizer BMS-806 with Trx1 nor Trx1 alone caused lysis. Order of addition experiments reinforced conformational activation/disulfide trigger as a sequential process, with virus/activator preincubation transiently enhancing lysis and virus/Trx1 preincubation reducing lysis. Lastly, addition of exogenous Trx1 to typical pseudovirus infections exhibited dose-dependent enhancement of infection. Altogether, these data support conformational activation and disulfide triggering as a mechanism that can induce and enhance the fusogenic transformation of Env.

Keywords: HIV-1 ; Env ; thioredoxins ; allosteric disulfides ; virus entry ; entry inhibitors ; thiols ; disulfide exchange

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Product Details of L-Ascorbic acid sodium salt

CAS No. :134-03-2
Formula : C6H7NaO6
M.W : 198.11
SMILES Code : [O-]C([C@@]1([H])[C@H](CO)O)=C(O)C(O1)=O.[Na+]
Synonyms :
Sodium ascorbate; Sodium L-ascorbate; Sodium Vitamin C
MDL No. :MFCD00082340
InChI Key :PPASLZSBLFJQEF-RXSVEWSESA-M
Pubchem ID :23667548

Safety of L-Ascorbic acid sodium salt

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P301+P312-P302+P352-P304+P340-P305+P351+P338

Isoform Comparison

Biological Activity

Description
L-Ascorbic acid sodium salt (Sodium ascorbate) functions as an electron donor and serves as an endogenous antioxidant. This compound specifically inhibits Cav3.2 channels with an IC50 of 6.5 μM, and it is noted for its roles in enhancing collagen deposition and inhibiting elastogenesis[1].[2].[3].

Protocol

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2.52mL

50.48mL

10.10mL

5.05mL

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