Glioblastoma-patient-derived cells	12 pM to 25 nM	48 hours	NSC319726 induces oxidative stress and cell cycle arrest by binding copper	Cell Chem Biol. 2018 May 17;25(5):585-594.e7.
MEF p53R172H/R172H	1 µM	24 hours	evaluates the growth inhibitory effect of NSC319726 on mouse p53R172H mutant cells	Cancer Cell. 2012 May 15;21(5):614-625.
SKOV3 (p53−/−)	1 µM	24 hours	evaluates the growth inhibitory effect of NSC319726 on p53 null cells	Cancer Cell. 2012 May 15;21(5):614-625.
OVCAR3 (p53R248W)	1 µM	24 hours	evaluates the growth inhibitory effect of NSC319726 on p53R248W mutant cells	Cancer Cell. 2012 May 15;21(5):614-625.
TOV112D (p53R175H)	1 µM	24 hours	induces a WT-like conformational change in the p53R175H mutant protein and apoptosis	Cancer Cell. 2012 May 15;21(5):614-625.
TOV112D	1 μM	72 hours	Evaluate the synergy of ZMC1 with cytotoxic chemotherapy or radiation, results showed that ZMC1 alone was effective but did not show synergy when combined with chemotherapy or radiation.	Mol Cancer Ther. 2019 Aug;18(8):1355-1365.
Escherichia coli	10 μg/mL	16-20 hours	To evaluate the antimicrobial activity of NSC319726 against E. coli AHDRCC 81113, showing an IC50 of 5.432 ± 2.731 μg/mL (0.0232 ± 0.012 μM).	Biomolecules. 2018 Dec 7;8(4):166.
TOV112D cells	1 μM	4 hours	Monitor ZMC1-induced refolding of p53 mutants	Elife. 2020 Dec 2;9:e61487.
H1299 cells	0.002 μM – 0.005 μM		Test the cell-killing effect of ZMC1 on p53 mutants	Elife. 2020 Dec 2;9:e61487.
H460	>2 μM	3 days	Assessed cell growth inhibition, showing low toxicity to p53 wild-type cells	Mol Pharmacol. 2017 Jun;91(6):567-575.
H1299	>2 μM	3 days	Assessed cell growth inhibition, showing low toxicity to p53 null cells	Mol Pharmacol. 2017 Jun;91(6):567-575.
HOP92	0.291 μM	3 days	Assessed cell growth inhibition, showing specific toxicity to p53-R175L mutant cells	Mol Pharmacol. 2017 Jun;91(6):567-575.
SKBR3	0.009 μM	3 days	Assessed cell growth inhibition, showing specific toxicity to p53-R175H mutant cells	Mol Pharmacol. 2017 Jun;91(6):567-575.
TOV112D	0.087 μM	3 days	Assessed cell growth inhibition, showing specific toxicity to p53-R175H mutant cells	Mol Pharmacol. 2017 Jun;91(6):567-575.
HT-1080 fibrosarcoma cells	31 nM	48 hours	NSC319726 induces cell growth inhibition via a copper-dependent mechanism	Cell Chem Biol. 2018 May 17;25(5):585-594.e7.
HCT116 cells	1 μM		To evaluate the effect of NSC319726 on cell viability, results showed that NSC319726 reduced cell survival.	Cell Biol Toxicol. 2024 May 1;40(1):26.
UOK276 cells	1 nM or 10 nM	90 hours	Assessed the effect of NSC319726 on UOK276 cell invasion, showing dose-dependent suppression of invasion.	Genes Chromosomes Cancer. 2017 Oct;56(10):719-729.
UOK276 cells	10 nM	5 days	Evaluated the effect of NSC319726 on UOK276 cell growth, showing approximately 70% reduction in growth over 5 days.	Genes Chromosomes Cancer. 2017 Oct;56(10):719-729.

Mice	P53R172H/R172H knock-in mice	Intraperitoneal injection	10 mg/kg/day or 5 mg/kg/day	Daily for up to 7 days	Evaluates the toxicity and therapeutic effect of NSC319726 on p53R172H mutant mice, showing p53R172H/R172H mice are more sensitive to NSC319726	Cancer Cell. 2012 May 15;21(5):614-625.
Nude mice NCR nu/nu	TOV112D xenograft model	Intraperitoneal injection	2.5 mg/kg	Daily for 17 days	Evaluate the in vivo synergy of ZMC1 with Nutlin 3a or ABT-199, results showed that ZMC1 combined with Nutlin 3a or ABT-199 significantly inhibited tumor growth.	Mol Cancer Ther. 2019 Aug;18(8):1355-1365.
Nude mice	Xenograft tumor model	Intraperitoneal injection	5 mg/kg	Daily	Test the efficacy of ZMC1 on p53 stability mutants in vivo	Elife. 2020 Dec 2;9:e61487.
Galleria mellonella larvae	C. auris infection model	Injection	6 and 12 mg/kg	Single dose, observed for 72 hours	Evaluation of the efficacy of NSC319726 in an invertebrate infection model, showing significantly improved survival rates compared to the untreated group	Microbiol Spectr. 2021 Dec 22;9(3):e0139521
ICR mice	Male reproductive system injury model	Intraperitoneal injection	1 mg/kg	Once daily for 5 weeks	To evaluate the toxicity of NSC319726 on the male reproductive system, results showed that NSC319726 caused testis index reduction, decreased sperm count, increased sperm malformation rate, structural damage in the testis and epididymis, impaired blood-testis barrier integrity, and decreased testosterone levels.	Cell Biol Toxicol. 2024 May 1;40(1):26.

Bio Calculators
Preparing Stock Solutions		1mg	5mg	10mg
Preparing Stock Solutions	1 mM 5 mM 10 mM	4.27mL 0.85mL 0.43mL	21.34mL 4.27mL 2.13mL	42.68mL 8.54mL 4.27mL
Dissolving Methods	Animal experiment Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn: in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day; The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound. Protocol 1 Protocol 2

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CAS No.: 71555-25-4

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CAS No.: 71555-25-4

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Precautionary Statements-General

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Precautionary Statements-General
Code	Phrase
P101	If medical advice is needed,have product container or label at hand.
P102	Keep out of reach of children.
P103	Read label before use
Prevention
Code	Phrase
P201	Obtain special instructions before use.
P202	Do not handle until all safety precautions have been read and understood.
P210	Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
P211	Do not spray on an open flame or other ignition source.
P220	Keep/Store away from clothing/combustible materials.
P221	Take any precaution to avoid mixing with combustibles
P222	Do not allow contact with air.
P223	Keep away from any possible contact with water, because of violent reaction and possible flash fire.
P230	Keep wetted
P231	Handle under inert gas.
P232	Protect from moisture.
P233	Keep container tightly closed.
P234	Keep only in original container.
P235	Keep cool
P240	Ground/bond container and receiving equipment.
P241	Use explosion-proof electrical/ventilating/lighting/equipment.
P242	Use only non-sparking tools.
P243	Take precautionary measures against static discharge.
P244	Keep reduction valves free from grease and oil.
P250	Do not subject to grinding/shock/friction.
P251	Pressurized container: Do not pierce or burn, even after use.
P260	Do not breathe dust/fume/gas/mist/vapours/spray.
P261	Avoid breathing dust/fume/gas/mist/vapours/spray.
P262	Do not get in eyes, on skin, or on clothing.
P263	Avoid contact during pregnancy/while nursing.
P264	Wash hands thoroughly after handling.
P265	Wash skin thouroughly after handling.
P270	Do not eat, drink or smoke when using this product.
P271	Use only outdoors or in a well-ventilated area.
P272	Contaminated work clothing should not be allowed out of the workplace.
P273	Avoid release to the environment.
P280	Wear protective gloves/protective clothing/eye protection/face protection.
P281	Use personal protective equipment as required.
P282	Wear cold insulating gloves/face shield/eye protection.
P283	Wear fire/flame resistant/retardant clothing.
P284	Wear respiratory protection.
P285	In case of inadequate ventilation wear respiratory protection.
P231 + P232	Handle under inert gas. Protect from moisture.
P235 + P410	Keep cool. Protect from sunlight.
Response
Code	Phrase
P301	IF SWALLOWED:
P304	IF INHALED:
P305	IF IN EYES:
P306	IF ON CLOTHING:
P307	IF exposed:
P308	IF exposed or concerned:
P309	IF exposed or if you feel unwell:
P310	Immediately call a POISON CENTER or doctor/physician.
P311	Call a POISON CENTER or doctor/physician.
P312	Call a POISON CENTER or doctor/physician if you feel unwell.
P313	Get medical advice/attention.
P314	Get medical advice/attention if you feel unwell.
P315	Get immediate medical advice/attention.
P320
P302 + P352	IF ON SKIN: wash with plenty of soap and water.
P321
P322
P330	Rinse mouth.
P331	Do NOT induce vomiting.
P332	IF SKIN irritation occurs:
P333	If skin irritation or rash occurs:
P334	Immerse in cool water/wrap n wet bandages.
P335	Brush off loose particles from skin.
P336	Thaw frosted parts with lukewarm water. Do not rub affected area.
P337	If eye irritation persists:
P338	Remove contact lenses, if present and easy to do. Continue rinsing.
P340	Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P341	If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P342	If experiencing respiratory symptoms:
P350	Gently wash with plenty of soap and water.
P351	Rinse cautiously with water for several minutes.
P352	Wash with plenty of soap and water.
P353	Rinse skin with water/shower.
P360	Rinse immediately contaminated clothing and skin with plenty of water before removing clothes.
P361	Remove/Take off immediately all contaminated clothing.
P362	Take off contaminated clothing and wash before reuse.
P363	Wash contaminated clothing before reuse.
P370	In case of fire:
P371	In case of major fire and large quantities:
P372	Explosion risk in case of fire.
P373	DO NOT fight fire when fire reaches explosives.
P374	Fight fire with normal precautions from a reasonable distance.
P376	Stop leak if safe to do so. Oxidising gases (section 2.4) 1
P377	Leaking gas fire: Do not extinguish, unless leak can be stopped safely.
P378
P380	Evacuate area.
P381	Eliminate all ignition sources if safe to do so.
P390	Absorb spillage to prevent material damage.
P391	Collect spillage. Hazardous to the aquatic environment
P301 + P310	IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P301 + P312	IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
P301 + P330 + P331	IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
P302 + P334	IF ON SKIN: Immerse in cool water/wrap in wet bandages.
P302 + P350	IF ON SKIN: Gently wash with plenty of soap and water.
P303 + P361 + P353	IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P304 + P312	IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell.
P304 + P340	IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P304 + P341	IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338	IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P306 + P360	IF ON CLOTHING: Rinse Immediately contaminated CLOTHING and SKIN with plenty of water before removing clothes.
P307 + P311	IF exposed: call a POISON CENTER or doctor/physician.
P308 + P313	IF exposed or concerned: Get medical advice/attention.
P309 + P311	IF exposed or if you feel unwell: call a POISON CENTER or doctor/physician.
P332 + P313	IF SKIN irritation occurs: Get medical advice/attention.
P333 + P313	IF SKIN irritation or rash occurs: Get medical advice/attention.
P335 + P334	Brush off loose particles from skin. Immerse in cool water/wrap in wet bandages.
P337 + P313	IF eye irritation persists: Get medical advice/attention.
P342 + P311	IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P370 + P376	In case of fire: Stop leak if safe to Do so.
P370 + P378	In case of fire:
P370 + P380	In case of fire: Evacuate area.
P370 + P380 + P375	In case of fire: Evacuate area. Fight fire remotely due to the risk of explosion.
P371 + P380 + P375	In case of major fire and large quantities: Evacuate area. Fight fire remotely due to the risk of explosion.
Storage
Code	Phrase
P401
P402	Store in a dry place.
P403	Store in a well-ventilated place.
P404	Store in a closed container.
P405	Store locked up.
P406	Store in corrosive resistant/ container with a resistant inner liner.
P407	Maintain air gap between stacks/pallets.
P410	Protect from sunlight.
P411
P412	Do not expose to temperatures exceeding 50 oC/ 122 oF.
P413
P420	Store away from other materials.
P422
P402 + P404	Store in a dry place. Store in a closed container.
P403 + P233	Store in a well-ventilated place. Keep container tightly closed.
P403 + P235	Store in a well-ventilated place. Keep cool.
P410 + P403	Protect from sunlight. Store in a well-ventilated place.
P410 + P412	Protect from sunlight. Do not expose to temperatures exceeding 50 oC/122oF.
P411 + P235	Keep cool.
Disposal
Code	Phrase
P501	Dispose of contents/container to ...
P502	Refer to manufacturer/supplier for information on recovery/recycling

Physical hazards
Code	Phrase
H200	Unstable explosive
H201	Explosive; mass explosion hazard
H202	Explosive; severe projection hazard
H203	Explosive; fire, blast or projection hazard
H204	Fire or projection hazard
H205	May mass explode in fire
H220	Extremely flammable gas
H221	Flammable gas
H222	Extremely flammable aerosol
H223	Flammable aerosol
H224	Extremely flammable liquid and vapour
H225	Highly flammable liquid and vapour
H226	Flammable liquid and vapour
H227	Combustible liquid
H228	Flammable solid
H229	Pressurized container: may burst if heated
H230	May react explosively even in the absence of air
H231	May react explosively even in the absence of air at elevated pressure and/or temperature
H240	Heating may cause an explosion
H241	Heating may cause a fire or explosion
H242	Heating may cause a fire
H250	Catches fire spontaneously if exposed to air
H251	Self-heating; may catch fire
H252	Self-heating in large quantities; may catch fire
H260	In contact with water releases flammable gases which may ignite spontaneously
H261	In contact with water releases flammable gas
H270	May cause or intensify fire; oxidizer
H271	May cause fire or explosion; strong oxidizer
H272	May intensify fire; oxidizer
H280	Contains gas under pressure; may explode if heated
H281	Contains refrigerated gas; may cause cryogenic burns or injury
H290	May be corrosive to metals
Health hazards
Code	Phrase
H300	Fatal if swallowed
H301	Toxic if swallowed
H302	Harmful if swallowed
H303	May be harmful if swallowed