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Chemical Structure| 1492-23-5 Chemical Structure| 1492-23-5

Structure of O-Succinyl-L-homoserine
CAS No.: 1492-23-5

Chemical Structure| 1492-23-5

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O-Succinyl-L-homoserine is an amino acid derivative that serves as an intermediate in methionine synthesis in the biosynthetic process of bacteria such as Escherichia coli, with important biological functions.

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Product Citations

Product Citations

Xu, Yueqi ; Liu, Shaonan ; Gao, Jinmin ; Hai, Yang ;

Abstract: Pyridoxal 5′-phosphate (PLP)-dependent enzymes catalyze a diverse array of biochemical transformations, making them invaluable biocatalytic tools for the synthesis of complex bioactive compounds. Here, we report the biochemical characterization of LolC, a PLP-dependent γ-synthase involved in the biosynthesis of loline alkaloids. LolC catalyzes the formation of a Cγ-N bond between O-acetyl--homoserine (OAH) and L-proline, generating a diamino diacid intermediate. Our findings reveal that LolC exhibits strict specificity for proline and its analogues, contrasting with the substrate promiscuity of closely related Cγ-C bond-forming enzyme Fub7. Structural analysis, using an AlphaFold model, identifies key differences in the substrate entrance tunnel of LolC, which is amphiphilic and distinct from the hydrophobic tunnel in Fub7. A mutagenesis study further highlights the functional divergence of a key active site residue between these enzymes. These results provide new insights into the substrate specificity and catalytic function of LolC, offering a valuable comparison to Fub7 and advancing our understanding of PLP_x005f_x0002_dependent enzymes involved in γ-substitution reactions.

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Product Details of O-Succinyl-L-homoserine

CAS No. :1492-23-5
Formula : C8H13NO6
M.W : 219.19
SMILES Code : O=C(O)[C@@H](N)CCOC(CCC(O)=O)=O
MDL No. :MFCD00055782
InChI Key :GNISQJGXJIDKDJ-YFKPBYRVSA-N
Pubchem ID :439406

Safety of O-Succinyl-L-homoserine

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H312-H332
Precautionary Statements:P261-P264-P270-P271-P280-P301+P312-P302+P352-P304+P340-P330-P363-P501

Isoform Comparison

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Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

4.56mL

0.91mL

0.46mL

22.81mL

4.56mL

2.28mL

45.62mL

9.12mL

4.56mL

 

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