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Chemical Structure| 65-22-5 Chemical Structure| 65-22-5

Structure of Pyridoxal HCl
CAS No.: 65-22-5

Chemical Structure| 65-22-5

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Pyridoxal hydrochloride is an endogenous metabolite.

Synonyms: Pyridoxal (hydrochloride); Pyridoxal hydrochloride; HQ

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Product Citations

Product Citations

Humberto De Vitto ; Kafi K. J. Belfon ; Nandini Sharma ; Sarah Toay ; Jan Abendroth ; David. M. Dranow , et al.

Abstract: Thiamin and its phosphate derivatives are ubiquitous molecules involved as essential cofactors in many cellular processes. The de novo biosynthesis of thiamin employs the parallel synthesis of 4-methyl-5-(2-hydroxyethyl)thiazole (THZ-P) and 4-amino-2-methyl-5(diphosphooxymethyl) pyrimidine (HMP) pyrophosphate (HMP-PP), which are coupled to generate thiamin phosphate. Most organisms that can biosynthesize thiamin employ a kinase (HMPK or ThiD) to generate HMP-PP. In nearly all cases, this enzyme is bifunctional and can also salvage free HMP, producing HMP-P, the monophosphate precursor of HMP-PP. Here we present high-resolution crystal structures of an HMPK from Acinetobacter baumannii (AbHMPK), both unliganded and with pyridoxal 5-phosphate (PLP) noncovalently bound. Despite the similarity between HMPK and pyridoxal kinase enzymes, our kinetics analysis indicates that AbHMPK accepts HMP exclusively as a substrate and cannot turn over , , or nor does it display phosphatase activity. PLP does, however, act as a weak inhibitor of AbHMPK with an IC50 of 768 μM. Surprisingly, unlike other HMPKs, AbHMPK catalyzes only the phosphorylation of HMP and does not generate the diphosphate HMP-PP. This suggests that an additional kinase is present in A. baumannii, or an alternative mechanism is in operation to complete the biosynthesis of thiamin.

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Product Details of Pyridoxal HCl

CAS No. :65-22-5
Formula : C8H10ClNO3
M.W : 203.62
SMILES Code : O=CC1=C(CO)C=NC(C)=C1O.[H]Cl
Synonyms :
Pyridoxal (hydrochloride); Pyridoxal hydrochloride; HQ
MDL No. :MFCD00012809
InChI Key :FCHXJFJNDJXENQ-UHFFFAOYSA-N
Pubchem ID :6171

Safety of Pyridoxal HCl

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P305+P351+P338

Isoform Comparison

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

4.91mL

0.98mL

0.49mL

24.56mL

4.91mL

2.46mL

49.11mL

9.82mL

4.91mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2
The prepared working fluid is recommended to be prepared now and used up as soon as possible in a short period of time. The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
 

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