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Chemical Structure| 74317-53-6 Chemical Structure| 74317-53-6

Structure of Rhodamine B Hydrazide
CAS No.: 74317-53-6

Chemical Structure| 74317-53-6

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Rhodamine B Hydrazide is an excellent sulfite probe with colorless, non-fluorescent properties. Its fluorescence intensity correlates with sulfite concentration (5-800 ng/mL, detection limit: 1.4 ng/mL (3σ)). Sulfites generate superoxide radicals by reducing dissolved oxygen, which then react with Rhodamine B hydrazide to form a fluorescent product, Rhodamine B, with maximum absorption at 554 nm and maximum emission at 574 nm. Fluorescence is enhanced by Tween 80 micelles.

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Product Details of Rhodamine B Hydrazide

CAS No. :74317-53-6
Formula : C28H32N4O2
M.W : 456.58
SMILES Code : O=C1N(N)C2(C3=C(OC4=C2C=CC(N(CC)CC)=C4)C=C(N(CC)CC)C=C3)C5=C1C=CC=C5
MDL No. :MFCD07808762
InChI Key :WTDHTIVYKKLOTC-UHFFFAOYSA-N
Pubchem ID :4205033

Safety of Rhodamine B Hydrazide

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302-H315-H319-H335
Precautionary Statements:P261-P264-P270-P271-P280-P301+P312-P302+P352-P304+P340-P305+P351+P338-P330-P332+P313-P337+P313-P362-P403+P233-P405-P501

Related Pathways of Rhodamine B Hydrazide

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Description
Rhodamine B hydrazide serves as an effective sulfite sensor, initially colorless and non-fluorescent. Its emission correlates with sulfite concentration (5-800 ng/mL; detection threshold at 1.4 ng/mL (3σ)). Sulfite converts dissolved oxygen into superoxide radicals, which react with Rhodamine B hydrazide, producing Rhodamine B and mimicking its fluorescence upon sulfite interaction, further amplified by Tween 80 surfactant micelles. Rhodamine B hydrazide exhibits peak absorption at 554 nm and fluorescence emission at 574 nm[1].

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