Name: Spermidine HCl | Spermidine trihydrochloride; 4-Azaoctamethylenediamine trihydrochloride
Brand: Ambeed
SKU: A881153
Rating: 4.3 (63 reviews)

CAS No. :	334-50-9
Formula :	C₇H₂₂Cl₃N₃
M.W :	254.63
SMILES Code :	NCCCCNCCCN.[H]Cl.[H]Cl.[H]Cl
Synonyms :	Spermidine trihydrochloride; 4-Azaoctamethylenediamine trihydrochloride; Spermidine hydrochloride
MDL No. :	MFCD00012918
InChI Key :	LCNBIHVSOPXFMR-UHFFFAOYSA-N
Pubchem ID :	9539

GHS Pictogram:
Signal Word:	Warning
Hazard Statements:	H315-H319
Precautionary Statements:	P305+P351+P338

Primary peritoneal macrophages	50 µM	1 hour	Spermidine inhibited NLRP3 and pro-IL-1β expression and ROS generation	Mol Med. 2022 Sep 4;28(1):103.
THP-1 macrophages	50 µM	1 hour	Spermidine inhibited LPS-induced NLRP3 inflammasome activation and IL-1β production, and reduced NF-κB phosphorylation	Mol Med. 2022 Sep 4;28(1):103.
BMDMs	10 mM	1 hourr	To evaluate the impact of spermidine on phagocytic uptake, results showed that 10 mM spermidine significantly increased the phagocytic uptake of non-motile mutants.	Elife. 2020 Sep 22;9:e55744.
Neonatal astrocytes	10 µM	18 hours	To assess the effect of spermidine on astrocyte inflammatory response. Results showed that spermidine reduced the poly I:C-induced release of IL-6, TNF-α, IL-10, IL-12, IL-4 and KC/GRO.	J Neuroinflammation. 2022 Jul 2;19(1):172.
RAW 264.7 cells	100 µM	24 hours	Evaluate cytocompatibility, results indicate good cytocompatibility up to 100 μM of SPD.	Adv Sci (Weinh). 2024 Jun;11(22):e2310162.
Human retinal pigment epithelial cells (ARPE-19)	10 µM	24 hours	Spermidine significantly attenuated H2O2-induced cytotoxicity, apoptosis, and G2/M phase cell cycle arrest, and reduced mitochondrial dysfunction.	Int J Mol Sci. 2021 Jan 29;22(3):1361.
H1299 cells	100 µM	4 hours and 16 hours	To evaluate the effect of spermidine on NRF2 and autophagy pathways. Results showed that SPD increased the protein levels of MAP1S, p62, and NRF2 in a dose-dependent manner, and decreased KEAP1 protein levels.	Hepatology. 2019 Jul;70(1):372-388.
Human vascular smooth muscle cells	0.1, 0.5, 1 µM	7 days	Spermidine significantly reduced mineral deposition and inhibited osteogenic differentiation markers BMP2 and Runx2 expression	Aging Cell. 2021 Jun;20(6):e13377.
Rat vascular smooth muscle cells	0.1, 0.5, 1 µM	7 days	Spermidine significantly reduced mineral deposition and inhibited osteogenic differentiation markers BMP2 and Runx2 expression	Aging Cell. 2021 Jun;20(6):e13377.

Drosophila	DSms/C0//C0 Drosophila	Oral	10 mM	Continuous administration	DFMO extended the lifespan of dSms/C0//C0 Drosophila, with median survival of male flies increasing from 21 to 35 days.	EMBO Mol Med. 2023 Nov 8;15(11):e17833
C57BL/6J mice	High-fat diet-induced obese Mice model	Oral	20 mg/kg	Once daily for 14 weeks	To evaluate the ameliorative effects of spermidine on metabolic syndrome, results showed spermidine significantly reduced body weight, improved lipid profiles and insulin sensitivity, and promoted white adipose tissue browning	Nat Commun. 2025 Jan 21;16(1):877
Sprague-Dawley rats	Middle-aged rat model	Drinking water	25 mg/kg	Daily administration for 350 days	To assess the effect of spermidine on behavioral performance and longevity of middle-aged rats. Results showed that spermidine did not significantly extend lifespan but improved body weight, kidney, liver, and heart morphology, reduced anxiety and increased exploratory behavior, enhanced brain autophagy, and decreased expression of inflammatory markers.	Geroscience. 2020 Jun;42(3):937-949
Rats	Subcutaneous implantation model	Subcutaneous implantation	250 μM	Single implantation, observed up to 21 days	Evaluate the immunomodulatory effects of DN-SPD hydrogel, results show DN-SPD hydrogel significantly reduced inflammatory responses and fibrous encapsulation.	Adv Sci (Weinh). 2024 Jun;11(22):e2310162.
Mice	CCl4-induced liver fibrosis model	Drinking water	3 mM	5 weeks	To assess the protective effect of spermidine on liver fibrosis. Results showed that SPD significantly alleviated CCl4-induced liver fibrosis, and this protective effect was significantly reduced in NRF2 or p62 single knockout mice and completely abolished in double knockout mice.	Hepatology. 2019 Jul;70(1):372-388.
Mice	APPPS1 mice	Oral	3 mM	Starting at 30 days of age until 120 or 290 days of age	To assess the effect of spermidine on AD pathology. Results showed that spermidine significantly reduced soluble Aβ40 and Aβ42 levels, decreased neuroinflammation, and altered microglial transcriptome and proteome.	J Neuroinflammation. 2022 Jul 2;19(1):172.
Rats	Chronic kidney disease model	Drinking water	3 mM	For 4 weeks	Spermidine significantly attenuated aortic calcification, reduced calcium content, and inhibited osteogenic differentiation markers BMP2 and Runx2 expression	Aging Cell. 2021 Jun;20(6):e13377.
C57BL/6 mice	Sepsis-induced acute kidney injury model	Drinking water	3 mM	Started 1 week before LPS treatment	Spermidine improved sepsis-induced acute kidney injury by modulating macrophage NLRP3 inflammasome activation and mitochondrial respiration	Mol Med. 2022 Sep 4;28(1):103.
Mice	ALS (G93A) transgenic mice	Intraperitoneal injection	5 mg/kg	4 times per week, from 30 to 120 days of age	Spermidine improved motor neuron survival and neuropathology in ALS mice, delayed disease onset, and prolonged lifespan	J Biomed Sci. 2022 Dec 20;29(1):106
C57BL/6 mice	Sepsis-induced acute kidney injury model	Intraperitoneal injection	50 mg/kg	Started 30 min or 24 h after LPS treatment, once daily	Spermidine improved sepsis-induced acute kidney injury by modulating macrophage NLRP3 inflammasome activation and mitochondrial respiration	Mol Med. 2022 Sep 4;28(1):103.
C57BL/6 mice	B16F10 melanoma model	Intraperitoneal injection	75 mg/kg	Single administration	Spermidine inhibited anti-CD3/CD28-induced CD8+ T cell activation and proliferation	Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2305245120

Bio Calculators
Preparing Stock Solutions		1mg	5mg	10mg
Preparing Stock Solutions	1 mM 5 mM 10 mM	3.93mL 0.79mL 0.39mL	19.64mL 3.93mL 1.96mL	39.27mL 7.85mL 3.93mL
Dissolving Methods	Animal experiment The prepared working fluid is recommended to be prepared now and used up as soon as possible in a short period of time. The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound. Protocol 1

Precautionary Statements-General
Code	Phrase
P101	If medical advice is needed,have product container or label at hand.
P102	Keep out of reach of children.
P103	Read label before use
Prevention
Code	Phrase
P201	Obtain special instructions before use.
P202	Do not handle until all safety precautions have been read and understood.
P210	Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
P211	Do not spray on an open flame or other ignition source.
P220	Keep/Store away from clothing/combustible materials.
P221	Take any precaution to avoid mixing with combustibles
P222	Do not allow contact with air.
P223	Keep away from any possible contact with water, because of violent reaction and possible flash fire.
P230	Keep wetted
P231	Handle under inert gas.
P232	Protect from moisture.
P233	Keep container tightly closed.
P234	Keep only in original container.
P235	Keep cool
P240	Ground/bond container and receiving equipment.
P241	Use explosion-proof electrical/ventilating/lighting/equipment.
P242	Use only non-sparking tools.
P243	Take precautionary measures against static discharge.
P244	Keep reduction valves free from grease and oil.
P250	Do not subject to grinding/shock/friction.
P251	Pressurized container: Do not pierce or burn, even after use.
P260	Do not breathe dust/fume/gas/mist/vapours/spray.
P261	Avoid breathing dust/fume/gas/mist/vapours/spray.
P262	Do not get in eyes, on skin, or on clothing.
P263	Avoid contact during pregnancy/while nursing.
P264	Wash hands thoroughly after handling.
P265	Wash skin thouroughly after handling.
P270	Do not eat, drink or smoke when using this product.
P271	Use only outdoors or in a well-ventilated area.
P272	Contaminated work clothing should not be allowed out of the workplace.
P273	Avoid release to the environment.
P280	Wear protective gloves/protective clothing/eye protection/face protection.
P281	Use personal protective equipment as required.
P282	Wear cold insulating gloves/face shield/eye protection.
P283	Wear fire/flame resistant/retardant clothing.
P284	Wear respiratory protection.
P285	In case of inadequate ventilation wear respiratory protection.
P231 + P232	Handle under inert gas. Protect from moisture.
P235 + P410	Keep cool. Protect from sunlight.
Response
Code	Phrase
P301	IF SWALLOWED:
P304	IF INHALED:
P305	IF IN EYES:
P306	IF ON CLOTHING:
P307	IF exposed:
P308	IF exposed or concerned:
P309	IF exposed or if you feel unwell:
P310	Immediately call a POISON CENTER or doctor/physician.
P311	Call a POISON CENTER or doctor/physician.
P312	Call a POISON CENTER or doctor/physician if you feel unwell.
P313	Get medical advice/attention.
P314	Get medical advice/attention if you feel unwell.
P315	Get immediate medical advice/attention.
P320
P302 + P352	IF ON SKIN: wash with plenty of soap and water.
P321
P322
P330	Rinse mouth.
P331	Do NOT induce vomiting.
P332	IF SKIN irritation occurs:
P333	If skin irritation or rash occurs:
P334	Immerse in cool water/wrap n wet bandages.
P335	Brush off loose particles from skin.
P336	Thaw frosted parts with lukewarm water. Do not rub affected area.
P337	If eye irritation persists:
P338	Remove contact lenses, if present and easy to do. Continue rinsing.
P340	Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P341	If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P342	If experiencing respiratory symptoms:
P350	Gently wash with plenty of soap and water.
P351	Rinse cautiously with water for several minutes.
P352	Wash with plenty of soap and water.
P353	Rinse skin with water/shower.
P360	Rinse immediately contaminated clothing and skin with plenty of water before removing clothes.
P361	Remove/Take off immediately all contaminated clothing.
P362	Take off contaminated clothing and wash before reuse.
P363	Wash contaminated clothing before reuse.
P370	In case of fire:
P371	In case of major fire and large quantities:
P372	Explosion risk in case of fire.
P373	DO NOT fight fire when fire reaches explosives.
P374	Fight fire with normal precautions from a reasonable distance.
P376	Stop leak if safe to do so. Oxidising gases (section 2.4) 1
P377	Leaking gas fire: Do not extinguish, unless leak can be stopped safely.
P378
P380	Evacuate area.
P381	Eliminate all ignition sources if safe to do so.
P390	Absorb spillage to prevent material damage.
P391	Collect spillage. Hazardous to the aquatic environment
P301 + P310	IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P301 + P312	IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
P301 + P330 + P331	IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
P302 + P334	IF ON SKIN: Immerse in cool water/wrap in wet bandages.
P302 + P350	IF ON SKIN: Gently wash with plenty of soap and water.
P303 + P361 + P353	IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P304 + P312	IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell.
P304 + P340	IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P304 + P341	IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338	IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P306 + P360	IF ON CLOTHING: Rinse Immediately contaminated CLOTHING and SKIN with plenty of water before removing clothes.
P307 + P311	IF exposed: call a POISON CENTER or doctor/physician.
P308 + P313	IF exposed or concerned: Get medical advice/attention.
P309 + P311	IF exposed or if you feel unwell: call a POISON CENTER or doctor/physician.
P332 + P313	IF SKIN irritation occurs: Get medical advice/attention.
P333 + P313	IF SKIN irritation or rash occurs: Get medical advice/attention.
P335 + P334	Brush off loose particles from skin. Immerse in cool water/wrap in wet bandages.
P337 + P313	IF eye irritation persists: Get medical advice/attention.
P342 + P311	IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P370 + P376	In case of fire: Stop leak if safe to Do so.
P370 + P378	In case of fire:
P370 + P380	In case of fire: Evacuate area.
P370 + P380 + P375	In case of fire: Evacuate area. Fight fire remotely due to the risk of explosion.
P371 + P380 + P375	In case of major fire and large quantities: Evacuate area. Fight fire remotely due to the risk of explosion.
Storage
Code	Phrase
P401
P402	Store in a dry place.
P403	Store in a well-ventilated place.
P404	Store in a closed container.
P405	Store locked up.
P406	Store in corrosive resistant/ container with a resistant inner liner.
P407	Maintain air gap between stacks/pallets.
P410	Protect from sunlight.
P411
P412	Do not expose to temperatures exceeding 50 oC/ 122 oF.
P413
P420	Store away from other materials.
P422
P402 + P404	Store in a dry place. Store in a closed container.
P403 + P233	Store in a well-ventilated place. Keep container tightly closed.
P403 + P235	Store in a well-ventilated place. Keep cool.
P410 + P403	Protect from sunlight. Store in a well-ventilated place.
P410 + P412	Protect from sunlight. Do not expose to temperatures exceeding 50 oC/122oF.
P411 + P235	Keep cool.
Disposal
Code	Phrase
P501	Dispose of contents/container to ...
P502	Refer to manufacturer/supplier for information on recovery/recycling

Physical hazards
Code	Phrase
H200	Unstable explosive
H201	Explosive; mass explosion hazard
H202	Explosive; severe projection hazard
H203	Explosive; fire, blast or projection hazard
H204	Fire or projection hazard
H205	May mass explode in fire
H220	Extremely flammable gas
H221	Flammable gas
H222	Extremely flammable aerosol
H223	Flammable aerosol
H224	Extremely flammable liquid and vapour
H225	Highly flammable liquid and vapour
H226	Flammable liquid and vapour
H227	Combustible liquid
H228	Flammable solid
H229	Pressurized container: may burst if heated
H230	May react explosively even in the absence of air
H231	May react explosively even in the absence of air at elevated pressure and/or temperature
H240	Heating may cause an explosion
H241	Heating may cause a fire or explosion
H242	Heating may cause a fire
H250	Catches fire spontaneously if exposed to air
H251	Self-heating; may catch fire
H252	Self-heating in large quantities; may catch fire
H260	In contact with water releases flammable gases which may ignite spontaneously
H261	In contact with water releases flammable gas
H270	May cause or intensify fire; oxidizer
H271	May cause fire or explosion; strong oxidizer
H272	May intensify fire; oxidizer
H280	Contains gas under pressure; may explode if heated
H281	Contains refrigerated gas; may cause cryogenic burns or injury
H290	May be corrosive to metals
Health hazards
Code	Phrase
H300	Fatal if swallowed
H301	Toxic if swallowed
H302	Harmful if swallowed
H303	May be harmful if swallowed
H304	May be fatal if swallowed and enters airways
H305	May be harmful if swallowed and enters airways
H310	Fatal in contact with skin
H311	Toxic in contact with skin
H312	Harmful in contact with skin
H313	May be harmful in contact with skin
H314	Causes severe skin burns and eye damage
H315	Causes skin irritation
H316	Causes mild skin irritation
H317	May cause an allergic skin reaction
H318	Causes serious eye damage
H319	Causes serious eye irritation
H320	Causes eye irritation
H330	Fatal if inhaled
H331	Toxic if inhaled
H332	Harmful if inhaled
H333	May be harmful if inhaled
H334	May cause allergy or asthma symptoms or breathing difficulties if inhaled
H335	May cause respiratory irritation
H336	May cause drowsiness or dizziness
H340	May cause genetic defects
H341	Suspected of causing genetic defects
H350	May cause cancer
H351	Suspected of causing cancer
H360	May damage fertility or the unborn child
H361	Suspected of damaging fertility or the unborn child
H361d	Suspected of damaging the unborn child
H362	May cause harm to breast-fed children
H370	Causes damage to organs
H371	May cause damage to organs
H372	Causes damage to organs through prolonged or repeated exposure
H373	May cause damage to organs through prolonged or repeated exposure
Environmental hazards
Code	Phrase
H400	Very toxic to aquatic life
H401	Toxic to aquatic life
H402	Harmful to aquatic life
H410	Very toxic to aquatic life with long-lasting effects
H411	Toxic to aquatic life with long-lasting effects
H412	Harmful to aquatic life with long-lasting effects
H413	May cause long-lasting harmful effects to aquatic life
H420	Harms public health and the environment by destroying ozone in the upper atmosphere

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CAS No.: 334-50-9

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CAS No.: 334-50-9

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