CAS No. :	1184-78-7
Formula :	C₃H₉NO
M.W :	75.11
SMILES Code :	C[N+](C)([O-])C
Synonyms :	NSC 408426; TMAO
MDL No. :	MFCD00002048
InChI Key :	UYPYRKYUKCHHIB-UHFFFAOYSA-N
Pubchem ID :	1145

GHS Pictogram:
Signal Word:	Warning
Hazard Statements:	H315-H319-H335
Precautionary Statements:	P261-P264-P280-P304+P340+P312-P337+P313-P403+P233

MC3T3-E1 osteoblasts	50 µM, 100 µM, 200 µM, 300 µM	1 day or 18 days	To investigate the effect of TMAO on osteoblast proliferation and differentiation, results showed that TMAO dose-dependently inhibited osteoblast proliferation and mineralized matrix production.	Cell Mol Life Sci. 2024 Dec 24;82(1):13
Human umbilical vein endothelial cells (HUVEC)	400 µM	12 hours	To investigate the effect of TMAO on oxidative stress and inflammatory injury in HUVECs. Results showed that TMAO promoted oxidative stress and inflammatory injury by activating the MAPK/Nrf2-Keap1 signaling pathway, inhibiting HUVEC migration and angiogenesis.	Gut Microbes. 2024 Jan-Dec;16(1):2311888
HCMEC/D3 cells	0.4 µM - 40 µM	24 hours	To evaluate the effects of TMA and TMAO on BBB integrity. TMA increased paracellular permeability, while TMAO at physiological concentrations reduced permeability and enhanced TEER.	Microbiome. 2021 Nov 27;9(1):235
Mouse vascular smooth muscle cells (VSMC)	100 µM	24 hours	TMAO enhances Ang II-induced intracellular Ca2+ release via activation of the PERK/ROS/CaMKII/PLCβ3 pathway.	Redox Biol. 2021 Oct;46:102115.
Human placental trophoblast cells (HTR-8/SVneo)	200 µM	48 hours	To investigate the effect of TMAO on oxidative stress and inflammatory injury in HTR-8/SVneo cells. Results showed that TMAO promoted oxidative stress and inflammatory injury by activating the MAPK/Nrf2-Keap1 signaling pathway, inhibiting HTR-8/SVneo cell migration and invasion.	Gut Microbes. 2024 Jan-Dec;16(1):2311888
Mouse carotid artery	25 µM, 50 µM, 100 µM	60 minutes	To evaluate the effect of TMAO on endothelium-dependent dilation in mouse carotid arteries. Results showed that TMAO concentration-dependently impaired endothelium-dependent dilation, and this impairment was reversed by the superoxide dismutase mimetic TEMPOL.	Hypertension. 2020 Jul;76(1):101-112

Mice	Photochemical ischemic stroke model and Middle Cerebral Artery Occlusion (MCAO) model	Oral	0.12% (w/w) TMAO or 1.00% (w/w) choline	3 weeks	TMAO and choline supplementation significantly increased cerebral infarct size and exacerbated post-stroke functional deficits.	Cell Host Microbe. 2021 Jul 14;29(7):1199-1208. e5
Mice	Male C57BL/6N mice	Dietary supplementation	0.12% TMAO	6 months	To evaluate the effect of long-term dietary TMAO supplementation on vascular endothelial function in young mice. Results showed that TMAO supplementation impaired endothelium-dependent dilation, accompanied by increased vascular oxidative stress, and this impairment was reversed by the superoxide dismutase mimetic TEMPOL.	Hypertension. 2020 Jul;76(1):101-112
C57BL/6 mice	High-fat diet-induced obesity model	Oral	0.2% TMAO	Consecutive 3 months	To investigate the effect of TMAO on bone mass loss, results showed that TMAO accelerated bone mass loss, inhibited bone formation and promoted bone resorption.	Cell Mol Life Sci. 2024 Dec 24;82(1):13
Female Sprague-Dawley rats	High-fat diet-induced sarcopenic obesity model in natural aging rats	Oral administration via drinking water	1% (V/V) TMAO	Daily administration for 16 weeks	TMAO aggravates high-fat diet-induced sarcopenic obesity via the ROS-AKT/mTOR signaling pathway	J Adv Res. 2025 Apr;70:405-422
C57Bl/6 mice	Ang II-induced hypertension model	Drinking water	1% TMAO	For 14 days	TMAO aggravates Ang II-induced hypertension and enhances vasoconstriction via the PERK/ROS/CaMKII/PLCβ3 axis.	Redox Biol. 2021 Oct;46:102115.
C57Bl/6J mice	Inflammatory model	Intraperitoneal injection	1.8 mg/kg	Single injection or long-term administration in drinking water	To evaluate the effects of TMAO on BBB integrity and cognitive function. TMAO enhanced BBB integrity in acute inflammatory models and protected cognitive function during chronic inflammatory challenges.	Microbiome. 2021 Nov 27;9(1):235
C57BL/6J mice	Non-alcoholic steatohepatitis (NASH) model	Intraperitoneal injection	75 mg/kg/day	Once daily for three months	To evaluate the effect of TMAO on liver fibrosis in NASH model mice. Results showed that TMAO significantly alleviated liver fibrosis, improved liver function, and restored gut microbiota diversity.	Front Immunol. 2022 Aug 22;13:964477

Precautionary Statements-General
Code	Phrase
P101	If medical advice is needed,have product container or label at hand.
P102	Keep out of reach of children.
P103	Read label before use
Prevention
Code	Phrase
P201	Obtain special instructions before use.
P202	Do not handle until all safety precautions have been read and understood.
P210	Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
P211	Do not spray on an open flame or other ignition source.
P220	Keep/Store away from clothing/combustible materials.
P221	Take any precaution to avoid mixing with combustibles
P222	Do not allow contact with air.
P223	Keep away from any possible contact with water, because of violent reaction and possible flash fire.
P230	Keep wetted
P231	Handle under inert gas.
P232	Protect from moisture.
P233	Keep container tightly closed.
P234	Keep only in original container.
P235	Keep cool
P240	Ground/bond container and receiving equipment.
P241	Use explosion-proof electrical/ventilating/lighting/equipment.
P242	Use only non-sparking tools.
P243	Take precautionary measures against static discharge.
P244	Keep reduction valves free from grease and oil.
P250	Do not subject to grinding/shock/friction.
P251	Pressurized container: Do not pierce or burn, even after use.
P260	Do not breathe dust/fume/gas/mist/vapours/spray.
P261	Avoid breathing dust/fume/gas/mist/vapours/spray.
P262	Do not get in eyes, on skin, or on clothing.
P263	Avoid contact during pregnancy/while nursing.
P264	Wash hands thoroughly after handling.
P265	Wash skin thouroughly after handling.
P270	Do not eat, drink or smoke when using this product.
P271	Use only outdoors or in a well-ventilated area.
P272	Contaminated work clothing should not be allowed out of the workplace.
P273	Avoid release to the environment.
P280	Wear protective gloves/protective clothing/eye protection/face protection.
P281	Use personal protective equipment as required.
P282	Wear cold insulating gloves/face shield/eye protection.
P283	Wear fire/flame resistant/retardant clothing.
P284	Wear respiratory protection.
P285	In case of inadequate ventilation wear respiratory protection.
P231 + P232	Handle under inert gas. Protect from moisture.
P235 + P410	Keep cool. Protect from sunlight.
Response
Code	Phrase
P301	IF SWALLOWED:
P304	IF INHALED:
P305	IF IN EYES:
P306	IF ON CLOTHING:
P307	IF exposed:
P308	IF exposed or concerned:
P309	IF exposed or if you feel unwell:
P310	Immediately call a POISON CENTER or doctor/physician.
P311	Call a POISON CENTER or doctor/physician.
P312	Call a POISON CENTER or doctor/physician if you feel unwell.
P313	Get medical advice/attention.
P314	Get medical advice/attention if you feel unwell.
P315	Get immediate medical advice/attention.
P320
P302 + P352	IF ON SKIN: wash with plenty of soap and water.
P321
P322
P330	Rinse mouth.
P331	Do NOT induce vomiting.
P332	IF SKIN irritation occurs:
P333	If skin irritation or rash occurs:
P334	Immerse in cool water/wrap n wet bandages.
P335	Brush off loose particles from skin.
P336	Thaw frosted parts with lukewarm water. Do not rub affected area.
P337	If eye irritation persists:
P338	Remove contact lenses, if present and easy to do. Continue rinsing.
P340	Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P341	If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P342	If experiencing respiratory symptoms:
P350	Gently wash with plenty of soap and water.
P351	Rinse cautiously with water for several minutes.
P352	Wash with plenty of soap and water.
P353	Rinse skin with water/shower.
P360	Rinse immediately contaminated clothing and skin with plenty of water before removing clothes.
P361	Remove/Take off immediately all contaminated clothing.
P362	Take off contaminated clothing and wash before reuse.
P363	Wash contaminated clothing before reuse.
P370	In case of fire:
P371	In case of major fire and large quantities:
P372	Explosion risk in case of fire.
P373	DO NOT fight fire when fire reaches explosives.
P374	Fight fire with normal precautions from a reasonable distance.
P376	Stop leak if safe to do so. Oxidising gases (section 2.4) 1
P377	Leaking gas fire: Do not extinguish, unless leak can be stopped safely.
P378
P380	Evacuate area.
P381	Eliminate all ignition sources if safe to do so.
P390	Absorb spillage to prevent material damage.
P391	Collect spillage. Hazardous to the aquatic environment
P301 + P310	IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P301 + P312	IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
P301 + P330 + P331	IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
P302 + P334	IF ON SKIN: Immerse in cool water/wrap in wet bandages.
P302 + P350	IF ON SKIN: Gently wash with plenty of soap and water.
P303 + P361 + P353	IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P304 + P312	IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell.
P304 + P340	IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P304 + P341	IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338	IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P306 + P360	IF ON CLOTHING: Rinse Immediately contaminated CLOTHING and SKIN with plenty of water before removing clothes.
P307 + P311	IF exposed: call a POISON CENTER or doctor/physician.
P308 + P313	IF exposed or concerned: Get medical advice/attention.
P309 + P311	IF exposed or if you feel unwell: call a POISON CENTER or doctor/physician.
P332 + P313	IF SKIN irritation occurs: Get medical advice/attention.
P333 + P313	IF SKIN irritation or rash occurs: Get medical advice/attention.
P335 + P334	Brush off loose particles from skin. Immerse in cool water/wrap in wet bandages.
P337 + P313	IF eye irritation persists: Get medical advice/attention.
P342 + P311	IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P370 + P376	In case of fire: Stop leak if safe to Do so.
P370 + P378	In case of fire:
P370 + P380	In case of fire: Evacuate area.
P370 + P380 + P375	In case of fire: Evacuate area. Fight fire remotely due to the risk of explosion.
P371 + P380 + P375	In case of major fire and large quantities: Evacuate area. Fight fire remotely due to the risk of explosion.
Storage
Code	Phrase
P401
P402	Store in a dry place.
P403	Store in a well-ventilated place.
P404	Store in a closed container.
P405	Store locked up.
P406	Store in corrosive resistant/ container with a resistant inner liner.
P407	Maintain air gap between stacks/pallets.
P410	Protect from sunlight.
P411
P412	Do not expose to temperatures exceeding 50 oC/ 122 oF.
P413
P420	Store away from other materials.
P422
P402 + P404	Store in a dry place. Store in a closed container.
P403 + P233	Store in a well-ventilated place. Keep container tightly closed.
P403 + P235	Store in a well-ventilated place. Keep cool.
P410 + P403	Protect from sunlight. Store in a well-ventilated place.
P410 + P412	Protect from sunlight. Do not expose to temperatures exceeding 50 oC/122oF.
P411 + P235	Keep cool.
Disposal
Code	Phrase
P501	Dispose of contents/container to ...
P502	Refer to manufacturer/supplier for information on recovery/recycling

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CAS No.: 1184-78-7

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CAS No.: 1184-78-7

Alternative Products

Product Details of Trimethylamine N-oxide

Safety of Trimethylamine N-oxide

Isoform Comparison

Biological Activity

Protocol

References

Historical Records

Precautionary Statements-General

Prevention

Response

Storage

Disposal

Physical hazards

Health hazards

Environmental hazards

Inquiry

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Physical hazards
Code	Phrase
H200	Unstable explosive
H201	Explosive; mass explosion hazard
H202	Explosive; severe projection hazard
H203	Explosive; fire, blast or projection hazard
H204	Fire or projection hazard
H205	May mass explode in fire
H220	Extremely flammable gas
H221	Flammable gas
H222	Extremely flammable aerosol
H223	Flammable aerosol
H224	Extremely flammable liquid and vapour
H225	Highly flammable liquid and vapour
H226	Flammable liquid and vapour
H227	Combustible liquid
H228	Flammable solid
H229	Pressurized container: may burst if heated
H230	May react explosively even in the absence of air
H231	May react explosively even in the absence of air at elevated pressure and/or temperature
H240	Heating may cause an explosion
H241	Heating may cause a fire or explosion
H242	Heating may cause a fire
H250	Catches fire spontaneously if exposed to air
H251	Self-heating; may catch fire
H252	Self-heating in large quantities; may catch fire
H260	In contact with water releases flammable gases which may ignite spontaneously
H261	In contact with water releases flammable gas
H270	May cause or intensify fire; oxidizer
H271	May cause fire or explosion; strong oxidizer
H272	May intensify fire; oxidizer
H280	Contains gas under pressure; may explode if heated
H281	Contains refrigerated gas; may cause cryogenic burns or injury
H290	May be corrosive to metals
Health hazards
Code	Phrase
H300	Fatal if swallowed
H301	Toxic if swallowed
H302	Harmful if swallowed
H303	May be harmful if swallowed