Type	HazMat fee for 500 gram (Estimated)
Excepted Quantity	USD 0.00
Limited Quantity	USD 15-60
Inaccessible (Haz class 6.1), Domestic	USD 80+
Inaccessible (Haz class 6.1), International	USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic	USD 100+
Accessible (Haz class 3, 4, 5 or 8), International	USD 200+

CAS No. :	11089-65-9
Formula :	C₃₇H₆₀N₄O₁₆
M.W :	816.89
SMILES Code :	NONE
Synonyms :	Tunicamycin Mixture
MDL No. :	MFCD00065709

GHS Pictogram:
Signal Word:	Danger
Hazard Statements:	H300
Precautionary Statements:	P264-P301+P310
Class:	6.1
UN#:	3462
Packing Group:	Ⅱ

Caco-2 cells	5 µg/ml	24 hours	To validate the screening results, reducing ATF6-dependent ERSE reporter gene activity	Gastroenterology. 2020 Oct;159(4):1357-1374.e10.
HEK293 cells	5 µg/ml	24 hours	To identify modulators of the ATF6 α signaling pathway	Gastroenterology. 2020 Oct;159(4):1357-1374.e10.
A1847 cells	5 μg/ml	8 hours	To investigate the regulatory role of CARM1 on XBP1s target gene expression during ER stress response	Nat Commun. 2021 Sep 7;12(1):5321.
MC38 epithelial cells	1μM	4 h	Induce ER stress and promote AIEC MSL1 replication	Cell Rep. 2022 Nov 15;41(7):111637.
HEK-293T cells	1 µM	24 h	To detect abnormal N-glycosylation, it was found that inhibition of N-glycosylation stabilized the localization of Halo protein in the ER	Sci Adv. 2021 Jan 15;7(3):eabc6364.
A549 cells	1 µM	24 h	Induction of Halo1N fluorescence by inhibition of N-glycosylation validated Halo1N as a reliable indicator of abnormal N-glycosylation	Sci Adv. 2021 Jan 15;7(3):eabc6364.
SH-SY5Y neuroblastoma cells	1 μg/ml	48 h	To investigate the effect of Tunicamycin on ER stress-induced cell death in SH-SY5Y cells and found that flurbiprofen significantly inhibited this cell death.	EMBO Mol Med. 2014 Mar;6(3):335-46.
NIT-1 pancreatic β-cells	0.05 μg/mL	24 h	To investigate the effect of Tunicamycin on the viability of NIT-1 pancreatic β-cells, the results showed that Tunicamycin significantly decreased cell viability in a dose-dependent manner.	Antioxidants (Basel). 2021 Jun 3;10(6):901.
HepG2 cells	12 μM	6 h	Induction of ER stress, suppression of CYP7A1 expression, and increased expression of ABCB11 and ABCC3	Cell Mol Gastroenterol Hepatol. 2016 Dec 10;3(2):261-271.
CHO cells	500nM	24 h	To validate the function of the NLG reporter, showing increased bioluminescence in CHO cells after Tunicamycin treatment	Clin Cancer Res. 2010 Jun 15;16(12):3205-14.
293T cells	500nM		To validate the design of the NLG reporter, showing altered glycosylation status of Luc protein in 293T cells after Tunicamycin treatment	Clin Cancer Res. 2010 Jun 15;16(12):3205-14.
Huh7shCON and Huh7shXBP1 cells	5 μg/ml	6 h	To induce ER stress and investigate the role of XBP1 in ER stress-induced FGF21 expression. Results showed that despite XBP1 knockdown, Huh7shXBP1 cells could still induce FGF21 expression normally under ER stress.	Mol Metab. 2017 Dec;6(12):1616-1624.
MEF cells	3 μg/ml	48 h	To investigate the change of EVA1A expression under ER stress, the results showed a significant increase of EVA1A protein level in the wild type (WT) cells, but not in Chop knockout cells upon tunicamycin treatment.	Cell Death Dis. 2023 Jan 30;14(1):71.
HEK293 cells	3 μg/ml	48 h	To investigate the interaction between EVA1A and MCL1, the results showed a significant interaction of EVA1A with MCL1 in cells after tunicamycin treatment.	Cell Death Dis. 2023 Jan 30;14(1):71.
LSK cells	3 μg/ml	36 h	To investigate the change of EVA1A expression under ER stress, the results showed an enhanced EVA1A mRNA level in the LSK cells from the mice treated with tunicamycin.	Cell Death Dis. 2023 Jan 30;14(1):71.
Caco-2 cells	2 µg/ml	5 or 7 h	To study the effect of ER stress on HMGCS2 expression, results showed that TM significantly decreased HMGCS2 transcription	Front Immunol. 2023 Jun 30;14:1185517.
HT29 cells	2 µg/ml	5 or 7 h	To study the effect of ER stress on HMGCS2 expression, results showed that TM significantly decreased HMGCS2 transcription	Front Immunol. 2023 Jun 30;14:1185517.

Mice	Atg16l1ΔIEC mouse model	Intraperitoneal injection	1mg/kg body weight	Sacrificed at 0 h, 24 h, 48 h	To evaluate the role of ATF6α signaling pathway in intestinal inflammation	Gastroenterology. 2020 Oct;159(4):1357-1374.e10.
C57BL/6J mice	ER stress model	Intraperitoneal injection	0.5 mg/kg (acute), 0.1 mg/kg (chronic)	Single injection (acute), daily injections for 5 days (chronic)	Induction of ER stress, suppression of Cyp7a1 expression, increased expression of Abcb11 and Abcc3, and reduced hepatic bile acid content	Cell Mol Gastroenterol Hepatol. 2016 Dec 10;3(2):261-271.
Nude mice	D54 glioma xenograft model	Intraperitoneal injection	0.75mg/kg	Single dose, lasting several days	To evaluate the effect of Tunicamycin on NLG inhibition in vivo, showing that Tunicamycin significantly reduces EGFR and Met protein levels in D54 tumors and enhances tumor radiosensitivity	Clin Cancer Res. 2010 Jun 15;16(12):3205-14.
Mice	Hepatocyte-specific Xbp1 knockout mice (Xbp1LKO)	Intraperitoneal injection	0.5 mg/kg	Single injection, sacrificed 6 hours post-injection	To investigate the induction of FGF21 in hepatocyte-specific Xbp1 knockout mice under pharmacologic ER stress. Results showed that Xbp1LKO mice could still induce FGF21 expression normally under ER stress, indicating that Xbp1 is not required for ER stress-induced FGF21 expression.	Mol Metab. 2017 Dec;6(12):1616-1624.
Mice	WT and Eva1aF/F,Vav-Cre mice	Intraperitoneal injection	0.5 mg/kg	Every 3 days for 7 days	To investigate the change of EVA1A expression under ER stress and its effect on HSPCs, the results showed that Eva1a deficient mice had more LSK, LT-HSC, ST-HSC, MPP3, MPP4, CMP, and GMP cells in BM than that in WT mice after tunicamycin treatment.	Cell Death Dis. 2023 Jan 30;14(1):71.
C57BL/6 mice	Wild type and Xbp1DIEC mice	Intraperitoneal injection	10 µg/gr	Single injection, sacrificed at 24 hours	To study the effect of ER stress on HMGCS2 expression, results showed that TM significantly decreased HMGCS2 expression	Front Immunol. 2023 Jun 30;14:1185517.

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CAS No.: 11089-65-9

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CAS No.: 11089-65-9

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Product Details of Tunicamycin

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References

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Precautionary Statements-General

Prevention

Response

Storage

Disposal

Physical hazards

Health hazards

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Preparing Stock Solutions		1mg	5mg	10mg
Preparing Stock Solutions	1 mM 5 mM 10 mM	1.22mL 0.24mL 0.12mL	6.12mL 1.22mL 0.61mL	12.24mL 2.45mL 1.22mL
Dissolving Methods	Animal experiment Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn: in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day; The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound. Protocol 1 Protocol 2

Precautionary Statements-General
Code	Phrase
P101	If medical advice is needed,have product container or label at hand.
P102	Keep out of reach of children.
P103	Read label before use
Prevention
Code	Phrase
P201	Obtain special instructions before use.
P202	Do not handle until all safety precautions have been read and understood.
P210	Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
P211	Do not spray on an open flame or other ignition source.
P220	Keep/Store away from clothing/combustible materials.
P221	Take any precaution to avoid mixing with combustibles
P222	Do not allow contact with air.
P223	Keep away from any possible contact with water, because of violent reaction and possible flash fire.
P230	Keep wetted
P231	Handle under inert gas.
P232	Protect from moisture.
P233	Keep container tightly closed.
P234	Keep only in original container.
P235	Keep cool
P240	Ground/bond container and receiving equipment.
P241	Use explosion-proof electrical/ventilating/lighting/equipment.
P242	Use only non-sparking tools.
P243	Take precautionary measures against static discharge.
P244	Keep reduction valves free from grease and oil.
P250	Do not subject to grinding/shock/friction.
P251	Pressurized container: Do not pierce or burn, even after use.
P260	Do not breathe dust/fume/gas/mist/vapours/spray.
P261	Avoid breathing dust/fume/gas/mist/vapours/spray.
P262	Do not get in eyes, on skin, or on clothing.
P263	Avoid contact during pregnancy/while nursing.
P264	Wash hands thoroughly after handling.
P265	Wash skin thouroughly after handling.
P270	Do not eat, drink or smoke when using this product.
P271	Use only outdoors or in a well-ventilated area.
P272	Contaminated work clothing should not be allowed out of the workplace.
P273	Avoid release to the environment.
P280	Wear protective gloves/protective clothing/eye protection/face protection.
P281	Use personal protective equipment as required.
P282	Wear cold insulating gloves/face shield/eye protection.
P283	Wear fire/flame resistant/retardant clothing.
P284	Wear respiratory protection.
P285	In case of inadequate ventilation wear respiratory protection.
P231 + P232	Handle under inert gas. Protect from moisture.
P235 + P410	Keep cool. Protect from sunlight.
Response
Code	Phrase
P301	IF SWALLOWED:
P304	IF INHALED:
P305	IF IN EYES:
P306	IF ON CLOTHING:
P307	IF exposed:
P308	IF exposed or concerned:
P309	IF exposed or if you feel unwell:
P310	Immediately call a POISON CENTER or doctor/physician.
P311	Call a POISON CENTER or doctor/physician.
P312	Call a POISON CENTER or doctor/physician if you feel unwell.
P313	Get medical advice/attention.
P314	Get medical advice/attention if you feel unwell.
P315	Get immediate medical advice/attention.
P320
P302 + P352	IF ON SKIN: wash with plenty of soap and water.
P321
P322
P330	Rinse mouth.
P331	Do NOT induce vomiting.
P332	IF SKIN irritation occurs:
P333	If skin irritation or rash occurs:
P334	Immerse in cool water/wrap n wet bandages.
P335	Brush off loose particles from skin.
P336	Thaw frosted parts with lukewarm water. Do not rub affected area.
P337	If eye irritation persists:
P338	Remove contact lenses, if present and easy to do. Continue rinsing.
P340	Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P341	If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P342	If experiencing respiratory symptoms:
P350	Gently wash with plenty of soap and water.
P351	Rinse cautiously with water for several minutes.
P352	Wash with plenty of soap and water.
P353	Rinse skin with water/shower.
P360	Rinse immediately contaminated clothing and skin with plenty of water before removing clothes.
P361	Remove/Take off immediately all contaminated clothing.
P362	Take off contaminated clothing and wash before reuse.
P363	Wash contaminated clothing before reuse.
P370	In case of fire:
P371	In case of major fire and large quantities:
P372	Explosion risk in case of fire.
P373	DO NOT fight fire when fire reaches explosives.
P374	Fight fire with normal precautions from a reasonable distance.
P376	Stop leak if safe to do so. Oxidising gases (section 2.4) 1
P377	Leaking gas fire: Do not extinguish, unless leak can be stopped safely.
P378
P380	Evacuate area.
P381	Eliminate all ignition sources if safe to do so.
P390	Absorb spillage to prevent material damage.
P391	Collect spillage. Hazardous to the aquatic environment
P301 + P310	IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P301 + P312	IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
P301 + P330 + P331	IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
P302 + P334	IF ON SKIN: Immerse in cool water/wrap in wet bandages.
P302 + P350	IF ON SKIN: Gently wash with plenty of soap and water.
P303 + P361 + P353	IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P304 + P312	IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell.
P304 + P340	IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P304 + P341	IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338	IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P306 + P360	IF ON CLOTHING: Rinse Immediately contaminated CLOTHING and SKIN with plenty of water before removing clothes.
P307 + P311	IF exposed: call a POISON CENTER or doctor/physician.
P308 + P313	IF exposed or concerned: Get medical advice/attention.
P309 + P311	IF exposed or if you feel unwell: call a POISON CENTER or doctor/physician.
P332 + P313	IF SKIN irritation occurs: Get medical advice/attention.
P333 + P313	IF SKIN irritation or rash occurs: Get medical advice/attention.
P335 + P334	Brush off loose particles from skin. Immerse in cool water/wrap in wet bandages.
P337 + P313	IF eye irritation persists: Get medical advice/attention.
P342 + P311	IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P370 + P376	In case of fire: Stop leak if safe to Do so.
P370 + P378	In case of fire:
P370 + P380	In case of fire: Evacuate area.
P370 + P380 + P375	In case of fire: Evacuate area. Fight fire remotely due to the risk of explosion.
P371 + P380 + P375	In case of major fire and large quantities: Evacuate area. Fight fire remotely due to the risk of explosion.
Storage
Code	Phrase
P401
P402	Store in a dry place.
P403	Store in a well-ventilated place.
P404	Store in a closed container.
P405	Store locked up.
P406	Store in corrosive resistant/ container with a resistant inner liner.
P407	Maintain air gap between stacks/pallets.
P410	Protect from sunlight.
P411
P412	Do not expose to temperatures exceeding 50 oC/ 122 oF.
P413
P420	Store away from other materials.
P422
P402 + P404	Store in a dry place. Store in a closed container.
P403 + P233	Store in a well-ventilated place. Keep container tightly closed.
P403 + P235	Store in a well-ventilated place. Keep cool.
P410 + P403	Protect from sunlight. Store in a well-ventilated place.
P410 + P412	Protect from sunlight. Do not expose to temperatures exceeding 50 oC/122oF.
P411 + P235	Keep cool.
Disposal
Code	Phrase
P501	Dispose of contents/container to ...
P502	Refer to manufacturer/supplier for information on recovery/recycling

Physical hazards
Code	Phrase
H200	Unstable explosive
H201	Explosive; mass explosion hazard
H202	Explosive; severe projection hazard
H203	Explosive; fire, blast or projection hazard
H204	Fire or projection hazard
H205	May mass explode in fire
H220	Extremely flammable gas
H221	Flammable gas
H222	Extremely flammable aerosol
H223	Flammable aerosol
H224	Extremely flammable liquid and vapour
H225	Highly flammable liquid and vapour
H226	Flammable liquid and vapour
H227	Combustible liquid
H228	Flammable solid
H229	Pressurized container: may burst if heated
H230	May react explosively even in the absence of air
H231	May react explosively even in the absence of air at elevated pressure and/or temperature
H240	Heating may cause an explosion
H241	Heating may cause a fire or explosion
H242	Heating may cause a fire
H250	Catches fire spontaneously if exposed to air
H251	Self-heating; may catch fire
H252	Self-heating in large quantities; may catch fire
H260	In contact with water releases flammable gases which may ignite spontaneously
H261	In contact with water releases flammable gas
H270	May cause or intensify fire; oxidizer
H271	May cause fire or explosion; strong oxidizer
H272	May intensify fire; oxidizer
H280	Contains gas under pressure; may explode if heated
H281	Contains refrigerated gas; may cause cryogenic burns or injury
H290	May be corrosive to metals
Health hazards
Code	Phrase
H300	Fatal if swallowed
H301	Toxic if swallowed
H302	Harmful if swallowed
H303	May be harmful if swallowed