Products Cited in Publications Worldwide Regulation of Cell Adhesion on Physically Crosslinked Hydrogels Composed of Amino Acid-Based Polymers by Changing Elastic Modulus Using Shape Fix/Memory Properties

Regulation of Cell Adhesion on Physically Crosslinked Hydrogels Composed of Amino Acid-Based Polymers by Changing Elastic Modulus Using Shape Fix/Memory Properties

Shin-nosuke Nishimura; Tomoya Yoshida; Nobuyuki Higashi; Tomoyuki Koga

DOI:

Abstract

The elastic modulus of hydrogels is one of the most important factors forcontrolling cell fate. In this study, hydrogels composed ofpoly(N-acryloylglycinamide) (PNAGAm) grafted with arginine (R)–glycine(G)–aspartic acid (D)–serine (S) is designed without a chemicalcrosslinker. The hydrogels are prepared by the conventional radicalcopolymerization of N-acryloylglycinamide with a polymerizable RGDSpeptide. The grafting ratio is easily controlled by adjusting the feedcomposition for polymerization. The hydrogels exhibit thermo-responsivenessof the upper critical solution temperature type based on the PNAGAm moiety.This characteristic reflects the shape fix/memory properties of hydrogelsbecause of the reversible formation of multiple hydrogen bonds. The celladhesiveness of the hydrogels drastically improves in the presence of a smallamount of the graft. The hydrogels exhibit good biocompatibility; theadhered cells proliferate on the hydrogels, and macrophages do not showactivation or inflammation. Surfaces with different elastic modulus regionsare successfully constructed on the same hydrogel by using shapefix/memory properties, allowing the regulation of cell adhesion. This hydrogelsystem offers promising opportunities for the design and application offunctional cell scaffolds.

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