Products Cited in Publications Worldwide N-methyl-O-benzylhydroxylamine derivatives of sialylated oligosaccharides, their synthesis and separation with reversed-phase HPLC

N-methyl-O-benzylhydroxylamine derivatives of sialylated oligosaccharides, their synthesis and separation with reversed-phase HPLC

Thomas Norberg; Elisabet Kallin

DOI: PMID:

Abstract

The human milk trisaccharide 3′- sialyllactose was reacted with an excess of N-methyl-O-benzylhydroxylamine (MBHA) and the product 3′- sialyllactose-MBHA was isolated in high (91%) yield by solid-phase extraction. The isomeric trisaccharide 6′-sialyllactose-MBHA was also prepared, in this case by enzymatic sialylation of lactose-MBHA. A 50/50 mixture of the two sialyllactose-MBHA derivatives was easily separated by reversed-phase HPLC. The free oligosaccharides were recovered from their respective MBHA derivatives by acid hydrolysis.

Keywords

Derivatization ; Milk oligosaccharides ; Hydroxylamines ; Reversed-phase chromatography

Purchased from AmBeed

A102444022513-22-095%

O-Benzyl-N-methylhydroxylamine

152.0/10g

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